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In this work, we propose a structured illumination (SI) method based on a two-photon excitation (TPE) scanning laser beam. Advantages of TPE methods include optical sectioning, low photo-toxicity, and robustness in the face of sample…

Biological Physics · Physics 2016-09-16 Alex Bardales , Qinggele Li , Bingzhao Zhu , Xiaodong Tao , Marc Reinig , Joel Kubby

Current in vivo microscopy allows us detailed spatiotemporal imaging (3D+t) of complete organisms and offers insights into their development on the cellular level. Even though the imaging speed and quality is steadily improving,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-31 Sourabh Bhide , Ralf Mikut , Maria Leptin , Johannes Stegmaier

A low-temperature ultra-high vacuum scanning probe microscopy (SPM) system with molecular beam epitaxy capability and optical access was conceived, built, and tested in our lab. The design of the whole system is discussed here, with special…

Instrumentation and Detectors · Physics 2021-03-09 Ze-Bin Wu , Zhao-Yan Gao , Xi-Ya Chen , Yu-Qing Xing , Huan Yang , Geng Li , Ruisong Ma , Aiwei Wang , Jiahao Yan , Chengmin Shen , Shixuan Du , Qing Huan , Hong-Jun Gao

Structured illumination microscopy (SIM) uses a set of images captured with different illumination patterns to computationally reconstruct resolution beyond the diffraction limit. Here, we propose an alternative approach using a single…

Optics · Physics 2025-06-17 Ruiming Cao , Guanghan Meng , Laura Waller

This comprehensive review discusses the development of scanning electron microscopy and the application of this technology in different fields such as biology, nanobiotechnology and biomedical science. Besides being a tool for high…

Quantitative Methods · Quantitative Biology 2023-11-02 Aniruddha Acharya

Microscopes have morphed from purely optical instruments into motorized, robotic machines that form images on digital sensors rather than eyeballs. This continuing trend towards automation and digitization enables many new approaches to…

Other Quantitative Biology · Quantitative Biology 2020-05-04 Raghav Chhetri , Stephan Preibisch , Nico Stuurman

The Transfer Matrix Method (TMM) has become a prominent tool for the optical simulation of thin$-$film solar cells, particularly among researchers specializing in organic semiconductors and perovskite materials. As the commercial viability…

Structured illumination microscopy (SIM) achieves doubled spatial resolution by exciting the specimen with a high-contrast, high-frequency sinusoidal pattern. Such an excitation pattern can be generated by interference between multiple…

Optics · Physics 2020-07-15 Meiqi Li , Yaning Li , Wenhui Liu , Karl Zhanghao , Peng Xi

In this paper, we have designed a low-cost scanning tunneling microscope (STM) priced at 300 USD or 2000 CNY. This microscope is suitable for educational purposes and low-demand research imaging at the nanometer level. This microscope's…

Instrumentation and Detectors · Physics 2024-01-17 Weilin Ma

In recent years, image-scanning microscopy (ISM, also termed pixel-reassignment microscopy) has emerged as a technique that improves the resolution and signal-to-noise compared to confocal and widefield microscopy by employing a detector…

Optics · Physics 2023-04-19 Tal I. Sommer , Gil Weinberg , Ori Katz

This document explores the advantages of integrating open source software and practices in managing a scientific lab, emphasizing reproducibility and the avoidance of pitfalls. It details practical applications from website management using…

Computers and Society · Computer Science 2024-05-14 Julien Cohen-Adad

Real time computer control is an essential feature of scanning probe microscopes, which have become essential tools for the characterization and investigation of nanometer scale samples. Most commercial (and some open-source) scanning probe…

Mesoscale and Nanoscale Physics · Physics 2013-01-24 Venkat Chandrasekhar , Manan Mehta

A mini-PET style detector system is being developed for a plant imaging application with a compact array of silicon photomultipliers (SiPM) replacing position sensitive photomultipliers (PSPMT). In addition to compactness, the use of SiPMs…

Optimizing communication performance is imperative for large-scale computing because communication overheads limit the strong scalability of parallel applications. Today's network cards contain rather powerful processors optimized for data…

Distributed, Parallel, and Cluster Computing · Computer Science 2017-10-20 Torsten Hoefler , Salvatore Di Girolamo , Konstantin Taranov , Ryan E. Grant , Ron Brightwell

Light-sheet fluorescence microscopy (LSFM) is used to capture volume images of biological specimens. It offers high contrast deep inside densely fluorescence labelled samples, fast acquisition speed and minimal harmful effects on the…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Niklas Rottmayer , Claudia Redenbach , Florian Fahrbach

In the era of large time-domain spectro-photometric surveys, surface variations such as starspots, chemical inhomogeneities, pulsations, rotational distortions, and binary interactions can now be directly detected and modelled. Accurately…

Solar and Stellar Astrophysics · Physics 2025-11-17 M. Jabłońska , T. Różański , L. Casagrande , H. Shah , P. A. Kołaczek-Szymański , M. Rychlicki , Yuan-Sen Ting

Wireless networks are a common place nowadays and almost all of the modern devices support wireless communication in some form. These networks differ from more traditional computing systems due to the ad-hoc and spontaneous nature of…

Networking and Internet Architecture · Computer Science 2010-07-15 Yasir Arfat Malkani , Lachhman Das Dhomeja

As an analogue of electromagnetically induced transparency (EIT), plasmon-induced transparency (PIT) has been realized both in plasmonic metamaterial and waveguide structures. Via near-field coupling within unit cells, PIT with broadband…

Fluorescence lifetime imaging microscopy (FLIM) is a powerful tool for quantitative fluorescence imaging because fluorescence lifetime is independent of concentration of fluorescent molecules or excitation/detection efficiency and is robust…

Most modern optical display and sensing devices utilize a limited number of spectral units within the visible range, based on human color perception. In contrast, the rapid advancement of machine-based pattern recognition and spectral…