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Salient Object Detection (SOD) with deep learning often requires substantial computational resources and large annotated datasets, making it impractical for resource-constrained applications. Lightweight models address computational demands…

Computer Vision and Pattern Recognition · Computer Science 2025-04-16 Leonardo M. Joao , Jancarlo F. Gomes , Silvio J. F. Guimaraes , Ewa Kijak , Alexandre X. Falcao

Rapid, comprehensive, and accurate cell phenotyping without compromising viability, is crucial to many important biomedical applications, including stem-cell therapy, drug screening, and liquid biopsy. Typical image cytometry methods…

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

The plasmonic tip of a scanning tunnelling microscope (STM) is functionalized with a single fluorescent molecule and is scanned on a plasmonic substrate. The tunneling current flowing through the tip-molecule-substrate junction generates a…

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

Holotomography (HT) has revolutionized quantitative label-free 3D imaging, yet conventional lens-based implementations are fundamentally constrained in field-of-view (FOV) and imaging depth, limiting their utility for critical…

Fourier Ptychographic Microscopy (FPM) is an imaging procedure that overcomes the traditional limit on Space-Bandwidth Product (SBP) of conventional microscopes through computational means. It utilizes multiple images captured using a low…

Computer Vision and Pattern Recognition · Computer Science 2022-08-17 Atreyee Saha , Salman S Khan , Sagar Sehrawat , Sanjana S Prabhu , Shanti Bhattacharya , Kaushik Mitra

We present experimental demonstration of tilt-mirror assisted transmission structured illumination microscopy (tSIM) that offers a large field of view super resolution imaging. An assembly of custom-designed tilt-mirrors are employed as the…

Optical endoscopy plays a crucial role in minimally invasive medical diagnostics and therapeutic procedures. Nonetheless, state-of-the-art endoscopic zoom objectives are bulky, requiring multi-element lens actuation, which can limit…

Novel techniques are currently being developed and established for the accurate chemical analysis and detection of single cancer cells, single embryos and single seeds by Fourier Transform Near Infrared (FT-NIR) Microspectroscopy, Fourier…

Tissues and Organs · Quantitative Biology 2007-05-23 I. C. Baianu , D. Costescu , N. E. Hofmann , S. S. Korban

Integrated quantum photonics, i.e. the generation, manipulation and detection of quantum states of light in integrated photonic chips, is revolutionizing the field of quantum information in all applications, from communications to…

Quantum Physics · Physics 2021-10-13 Giacomo Corrielli , Andrea Crespi , Roberto Osellame

Near-field scanning optical microscopy has been an indispensable tool for designing, characterizing and understanding the functionalities of diverse nanoscale photonic devices. As the advances in fabrication technology have driven the…

Recent advances in cancer research largely rely on new developments in microscopic or molecular profiling techniques offering high level of detail with respect to either spatial or molecular features, but usually not both. Here, we present…

Following the recent developement of Fourier ptychographic microscopy (FPM) in the visible range by Zheng et al. (2013), we propose an adaptation for hard x-rays. FPM employs ptychographic reconstruction to merge a series of low-resolution,…

Instrumentation and Detectors · Physics 2016-09-27 H. Simons , H. F. Poulsen , J. P. Guigay , C. Detlefs

Super-resolution fluorescence microscopy overcomes blurring arising from light diffraction, allowing the reconstruction of fine scale details in biological structures. Standard methods come at the expense of long acquisition time and/or…

Optimization and Control · Mathematics 2021-10-12 Bastien Laville , Laure Blanc-Féraud , Gilles Aubert

We use fluorescent nanospheres as scalar detectors for the electric-field intensity in order to probe the near-field of an optical tip used in aperture-type near-field scanning optical microscopy (NSOM). Surprisingly, the recorded…

Optics · Physics 2007-05-23 A. Drezet , M. J. Nasse , S. Huant , J. C. Woehl

Fibered confocal fluorescence microscopy (FCFM) is a new technique that can be used during a bronchoscopy to analyze the nature of the human bronchial and alveolar mucosa fluorescence microstructure. An endoscopic fibered confocal…

Medical Physics · Physics 2008-02-27 G. Bourg-Heckly , L. Thiberville , C. Vever-Bizet , B. Viellerobe

We present an ultra-thin hybrid imaging system based on an optical multimode fiber (MMF) and an optical fiber hydrophone that combines optical resolution photoacoustic and fluorescence microscopy. To control the illumination at the distal…

We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…

Optical microscopy has a diffraction limited resolution of about 250 nm. Fluorescence methods (e.g. PALM, STORM, STED) beat this, but they are still limited to 10 s of nm, and the images are an indirect pointillist representation of only…

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