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In this paper we present a digital holographic microscope to track gold colloids in three dimensions. We report observations of 100nm gold particles in motion in water. The expected signal and the chosen method of reconstruction are…

Optics · Physics 2012-10-12 Frédéric Verpillat , Fadwa Joud , Pierre Desbiolles , Michel Gross

The 3D structure of light scattering from dark-field illuminated live 3T3 cells marked with 40 nm gold nanomarkers is explored. For this purpose, we use a high resolution holographic microscope combining the off-axis heterodyne geometry and…

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40…

Identifying, visualising and ultimately tracking dynamically moving non-fluorescent nanoparticles in the presence of non-specific scattering is a long-standing challenge across the nano- and life-sciences. In this work we demonstrate that…

We report experimental results on heterodyne holographic microscopy of subwavelength-sized gold particles. The apparatus uses continuous green laser illumination of the metal beads in a total internal reflection configuration for dark-field…

In-line digital holography is a valuable tool for sizing, locating and tracking micro- or nano-objects in a volume. When a parametric imaging model is available, Inverse Problems approaches provide a straightforward estimate of the object…

Instrumentation and Detectors · Physics 2023-07-19 Nicolas Verrier , Corinne Fournier , Thierry Fournel

In this paper, we explore the 3D structure of light scattering from dark-field illuminated live 3T3 cells in the presence of 40 nm gold nanomarkers. For this purpose, we use a high resolution holographic microscope combining the off-axis…

Specialized applications of nanoparticles often call for particular, well-characterized particle size distributions in solution. But, this property can prove difficult to measure. High-throughput methods, such as dynamic light scattering,…

Mesoscale and Nanoscale Physics · Physics 2021-09-24 Luke Melo , Angus Hui , Matt Kowal , Eric Boateng , Zahra Poursorkh , Edène Rocheron , Jake Wong , Ashton Christy , Edward Grant

Tracking nanoparticle movement is highly desirable in many scientific areas, and various imaging methods have been employed to achieve this goal. Interferometric scattering (iSCAT) microscopy has been particularly successful in combining…

Optics · Physics 2024-01-24 Kiarash Kasaian , Mahdi Mazaheri , Vahid Sandoghdar

We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…

Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…

Single nanoparticle tracking using optical microscopy is a powerful technique with many applications in biology, chemistry and material sciences. Despite significant advances, localising objects with nanometric position accuracy in a…

Advanced three-dimensional (3D) tracking methods are essential for studying particle dynamics across a wide range of complex systems, including multiphase flows, environmental and atmospheric sciences, colloidal science, biological and…

Fluid Dynamics · Physics 2024-12-25 Shyam Kumar M , Jiarong Hong

We present a new, high-speed technique to track the three-dimensional translation and rotation of non-spherical colloidal particles. We capture digital holograms of micrometer-scale silica rods and sub-micrometer-scale Janus particles…

A differential interference contrast microscopy technique, which employs a photonic crystal fiber as a white-light source, is used to measure both the real and imaginary parts of the complex dielectric constant of single 10 and 15 nm gold…

Materials Science · Physics 2009-11-11 Patrick Stoller , Volker Jacobsen , Vahid Sandoghdar

Monitoring chemical reactions in solutions at the scale of individual entities is challenging: single particle detection requires small confocal volumes which are hardly compatible with Brownian motion, particularly when long integration…

The paper presents a hybrid bubble hologram processing approach for measuring the size and 3D distribution of bubbles over a wide range of size and shape. The proposed method consists of five major steps, including image enhancement,…

Applied Physics · Physics 2019-04-25 Siyao Shao , Jiarong Hong

Particle size measurement based on digital holography with conventional algorithms are usually time-consuming and susceptible to noises associated with hologram quality and particle complexity, limiting its usage in a broad range of…

Applied Physics · Physics 2020-01-01 Siyao Shao , Kevin Mallery , Jiarong Hong

Quantitative polarisation-resolved optical extinction microscopy of individual plasmonic nanoparticles has recently been introduced as a powerful tool to characterise the nanoparticle's morphology with a precision comparable to electron…

Optics · Physics 2026-02-10 Lukas M Payne , Furqan Alabdullah , Paola Borri , Wolfgang Langbein
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