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Branched actin networks at the leading edge of a crawling cell evolve via protein-regulated processes such as polymerization, depolymerization, capping, branching, and severing. A formulation of these processes is presented and analyzed to…

Soft Condensed Matter · Physics 2009-11-13 Ajay Gopinathan , Kun-Chun Lee , J. M. Schwarz , Andrea J. Liu

The polymerization of actin via branching at a cell membrane containing nucleation-promoting factors is simulated using a stochastic-growth methodology. The polymerized-actin distribution displays three types of behavior: a) traveling…

Biological Physics · Physics 2015-05-19 Anders E. Carlsson

The actin cytoskeleton of adherent tissue cells often condenses into filament bundles contracted by myosin motors, so-called stress fibers, which play a crucial role in the mechanical interaction of cells with their environment. Stress…

Subcellular Processes · Quantitative Biology 2015-05-27 Achim Besser , Julien Colombelli , Ernst H. K. Stelzer , Ulrich S. Schwarz

A living cell actively generates traction forces on its environment with its actin cytoskeleton. These forces deform the cell elastic substrate which, in turn, affects the traction forces exerted by the cell and can consequently modify the…

Biological Physics · Physics 2022-02-03 H. Chelly , A. Jahangiri , M. Mireux , J. Étienne , D. K. Dysthe , C. Verdier , P. Recho

Gradual disruption of the actin cytoskeleton induces a series of structural shape changes in cells leading to a transformation of cylindrical cell extensions into a periodic chain of "pearls". Quantitative measurements of the pearling…

Biological Physics · Physics 2010-08-06 Roy Bar-Ziv , Tsvi Tlusty , Elisha Moses , Samuel A. Safran , Alexander Bershadsky

The origin of biological motion can be traced back to the function of molecular motor proteins. Cytoplasmic dynein and kinesin transport organelles within our cells moving along a polymeric filament, the microtubule. The motion of the…

Statistical Mechanics · Physics 2015-06-25 Imre Derenyi , Tamas Vicsek

Observations of single epidermal cells on flat adhesive substrates have revealed two distinct morphological and functional states, namely a non-migrating symmetric unpolarized state and a migrating asymmetric polarized state. These states…

Biological Physics · Physics 2010-04-14 Wolfgang Alt , Martin Bock , Christoph Möhl

The cytoskeleton relies on diverse populations of motors, filaments, and binding proteins acting in concert to enable non-equilibrium processes ranging from mitosis to chemotaxis. Its versatile reconfigurability, programmed by interactions…

Living systems often exhibit internal driving: active, molecular processes drive nonequilibrium phenomena such as metabolism or migration. Active gels constitute a fascinating class of internally driven matter, where molecular motors exert…

Biological Physics · Physics 2015-06-15 Jose Alvarado , Michael Sheinman , Abhinav Sharma , Fred C. MacKintosh , Gijsje H. Koenderink

Suspensions of swimming micro-organisms provide examples of coordinated active dynamics. That has stimulated the study of a phenomenological theory combining synchronization and polar order in active matter. Here, we consider another…

Soft Condensed Matter · Physics 2019-04-08 M. Leoni

The cooperative action of many molecular motors is essential for dynamic processes such as cell motility and mitosis. This action can be studied by using motility assays in which the motion of cytoskeletal filaments over a surface coated…

Soft Condensed Matter · Physics 2009-06-01 Barak Gilboa , David Gillo , Oded Farago , Anne Bernheim-Groswasser

Biological systems tightly regulate their physiological state using control signals. This includes the actomyosin cytoskeleton, a contractile active gel that consumes chemical free energy to drive many examples of cellular mechanical…

Soft Condensed Matter · Physics 2025-03-03 James Clarke , Francis Cavanna , Aniket Marne , Anthony Davolio , José Alvarado

The eukaryotic cell membrane is connected to a dense actin rich cortex. We present FCS and STED experiments showing that dense membrane bound actin networks have severe influence on lipid phase separation. A minimal actin cortex was bound…

Soft Condensed Matter · Physics 2018-10-17 Alf Honigmann , Sina Sadeghi , Jan Keller , Stefan W. Hell , Christian Eggeling , Richard Vink

Coordination of cytoplasmic flows on large scales in space and time are at the root of many cellular processes, including growth, migration or division. These flows are driven by organized contractions of the actomyosin cortex. In order to…

Adaptation and Self-Organizing Systems · Physics 2022-07-29 Busson Valentin , Saiseau Raphaël , Durand Marc

The actin cytoskeleton of cells is in continuous motion due to both polymerization of new filaments and their contraction by myosin II molecular motors. Through adhesion to the substrate, such intracellular flow can be converted into cell…

Subcellular Processes · Quantitative Biology 2021-08-11 Oliver M. Drozdowski , Falko Ziebert , Ulrich S. Schwarz

Polymerization of actin proteins into dynamic structures is essential to eukaryotic cell life. This has motivated a large body of in vitro experiments measuring polymerization kinetics of individual filaments. Here we model these kinetics,…

Biomolecules · Quantitative Biology 2009-11-10 Dimitrios Vavylonis , Qingbo Yang , Ben O'Shaughnessy

Biological cells exhibit a hierarchical spatial organization, where various compartments harbor condensates that form by phase separation. Cells can control the emergence of these condensates by affecting compartment size, the amount of the…

Biological Physics · Physics 2025-04-03 Riccardo Rossetto , Gerrit Wellecke , David Zwicker

Cell spreading requires a major reorganisation of the actin cytoskeleton, from a cortical structure to a lamellipodium where filaments are mostly parallel to the substrate. We propose a model inspired by the physics of nematic liquid…

Biological Physics · Physics 2019-06-07 Mathieu Dedenon , Pierre Sens

Structure formation and constant reorganization of the actin cytoskeleton are key requirements for the function of living cells. Here we show that a minimal reconstituted system consisting of actin filaments, crosslinking molecules and…

Biological Physics · Physics 2015-05-27 Simone Köhler , Volker Schaller , Andreas R. Bausch

Molecular motors are involved in key transport processes inside actin-based cellular protrusions. The motors carry cargo proteins to the protrusion tip which participate in regulating the actin polymerization, and play a key role in…

Biological Physics · Physics 2014-06-12 Itai Pinkoviezky , Nir S Gov
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