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Single-particle electron microscopy is a modern technique that biophysicists employ to learn the structure of proteins. It yields data that consist of noisy random projections of the protein structure in random directions, with the added…

Applications · Statistics 2012-03-01 Victor M. Panaretos , Kjell Konis

We demonstrate that powder diffraction data can be collected from sub-micron crystals of a mbrane protein with nearly two orders of magnitude more atoms than the molecules commonly used for powder diffraction. The crystals of photosystem-1…

Recovery of three-dimensional structure from single particle X-ray scattering of completely randomly oriented diffraction patterns as predicted few decades back has been real due to the advent of the new emerging X-ray Free Electron Laser…

Soft Condensed Matter · Physics 2015-11-24 M. Uddin

We present a simple 'shift-and-add' based improvement in the angular resolution of single electron backscatter diffraction (EBSD) patterns. Sub-pixel image registration is used to measure the (sub-pixel) difference in projection parameters…

Instrumentation and Detectors · Physics 2025-12-15 Ben Britton , Tianbi Zhang

Motivated by recent experimental initiatives, such as at the Helmholtz International Beamline for Extreme Fields (HIBEF) at the European X-ray Free Electron Laser (XFEL), we calculate the birefringent scattering of x-rays at the combined…

We introduce the EMC algorithm for reconstructing a particle's 3D diffraction intensity from very many photon shot-noise limited 2D measurements, when the particle orientation in each measurement is unknown. The algorithm combines a…

Data Analysis, Statistics and Probability · Physics 2010-03-04 Duane Ne-Te Loh , Veit Elser

Scanning X-ray nanodiffraction microscopy is a powerful technique for spatially resolving nanoscale structural morphologies by diffraction contrast. One of the critical challenges in experimental nanodiffraction data analysis is posed by…

Applied Physics · Physics 2024-06-26 Aileen Luo , Tao Zhou , Martin V. Holt , Andrej Singer , Mathew J. Cherukara

Schemes for X-ray imaging single protein molecules using new x-ray sources, like x-ray free electron lasers (XFELs), require processing many frames of data that are obtained by taking temporally short snapshots of identical molecules, each…

Data Analysis, Statistics and Probability · Physics 2015-06-17 Kartik Ayyer , Hugh T. Philipp , Mark W. Tate , Veit Elser , Sol M. Gruner

Diffraction-before-destruction imaging with single ultrashort X-ray pulses has the potential to visualise non-equilibrium processes, such as chemical reactions, at the nanoscale with sub-femtosecond resolution in the native environment…

We study the inverse problem of localization (imaging) of a laser beam from measurements of the intensity of light scattered off-axis by a Poisson cloud of small particles. Starting from the wave equation, we analyze the microscopic…

Data Analysis, Statistics and Probability · Physics 2017-07-27 Liliana Borcea , Josselin Garnier

The X-ray free electron lasers (XFEL) can enable diffractive structural determination of protein crystals or single molecules that are too radiation-sensitive for conventional X-ray analysis. However the electronic form factor could have…

Quantum Physics · Physics 2017-01-30 Zheng Li , Nikita Medvedev , Henry Chapman , Yanhua Shih

We demonstrate a smart laser-diffraction analysis technique for particle mixture identification. We retrieve information about the size, geometry, and ratio concentration of two-component heterogeneous particle mixtures with an efficiency…

Image and Video Processing · Electrical Eng. & Systems 2022-02-03 Arturo Villegas , Mario A. Quiroz-Juarez , Alfred U'Ren , Juan P. Torres , Roberto de J. Leon-Montiel

Imaging of the structure of single proteins or other biomolecules with atomic resolution would be enormously beneficial to structural biology. X-ray free-electron lasers generate highly intense and ultrashort x-ray pulses, providing a route…

Atomic Physics · Physics 2014-07-30 Jan Malte Slowik , Sang-Kil Son , Gopal Dixit , Zoltan Jurek , Robin Santra

A solution to the inversion problem of scattering would offer aberration-free diffraction-limited 3D images without the resolution and depth-of-field limitations of lens-based tomographic systems. Powerful algorithms are increasingly being…

Light detection and ranging (Lidar) single-photon devices capture range and intensity information from a 3D scene. This modality enables long range 3D reconstruction with high range precision and low laser power. A multispectral…

Signal Processing · Electrical Eng. & Systems 2019-09-20 Julián Tachella , Yoann Altmann , Miguel Márquez , Henry Arguello-Fuentes , Jean-Yves Tourneret , Stephen McLaughlin

Solving crystal structures from kinematical X-ray or electron diffraction patterns of single crystals requires many more diffracted beams to be recorded than there are atoms in the structure, since the phases of the structure factors can…

Materials Science · Physics 2023-02-13 Christoph T. Koch

Nuclear resonant x-ray diffraction in grazing incidence geometry is used to determine the lateral magnetic configuration in a one-dimensional lattice of ferromagnetic nanostripes. During magnetic reversal, strong nuclear superstructure…

We introduce a rapid data acquisition and reconstruction method to image the crystalline structure of materials and associated strain and orientations at micrometer resolution using Laue diffraction. Our method relies on scanning a…

Materials Science · Physics 2022-09-15 Doga Gursoy , Dina Sheyfer , Michael Wojcik , Wenjun Liu , Jonathan Z. Tischler
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