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Single-particle cryo-electron microscopy (cryo-EM) has recently joined X-ray crystallography and NMR spectroscopy as a high-resolution structural method to resolve biological macromolecules. In a cryo-EM experiment, the microscope produces…

Information Theory · Computer Science 2022-10-28 Tamir Bendory , Nicolas Boumal , William Leeb , Eitan Levin , Amit Singer

Very short pulses of x-ray free-electron lasers opened the way to obtain diffraction signal from single particles beyond the radiation dose limit. For 3D structure reconstruction many patterns are recorded in the object's unknown…

Data Analysis, Statistics and Probability · Physics 2011-05-11 Gábor Bortel , Miklós Tegze

This note compares the single-shot and intensity cross-correlation proposals for x-ray imaging of randomly oriented particles and shows very directly that the latter will usually not be feasible even when the former is.

Data Analysis, Statistics and Probability · Physics 2010-07-23 Veit Elser

We demonstrate that a common-line method can assemble a 3D oversampled diffracted intensity distribution suitable for high-resolution structure solution from a set of measured 2D diffraction patterns, as proposed in experiments with an…

Biomolecules · Quantitative Biology 2007-10-16 V. L. Shneerson , A. Ourmazd , D. K. Saldin

In this paper, we propose a new model to segment cells in phase contrast microscopy images. Cell images collected from the similar scenario share a similar background. Inspired by this, we separate cells from the background in images by…

Computer Vision and Pattern Recognition · Computer Science 2019-04-02 Lin Zhang

A deep understanding of molecular photo-transformations is challenging because of the complex interaction between the configurations of electrons and nuclei. An initial optical excitation dissipates energy into electronic and structural…

We reconstructed intensities in Fourier space and electron densities in the real space for an azimuthally symmetric object Nanorice particle (Iron Oxide nanoparticle) exposed in the ultrashort, bright and coherent x-ray free electron laser…

Biological Physics · Physics 2020-07-14 S. S. Kim , P. Nepal , D. K. Saldin , C. H. Yoon

We present a new Bayesian methodology to learn the unknown material density of a given sample by inverting its two-dimensional images that are taken with a Scanning Electron Microscope. An image results from a sequence of projections of the…

Applications · Statistics 2014-03-06 Dalia Chakrabarty , Fabio Rigat , Nare Gabrielyan , Richard Beanland , Shashi Paul

A practical method utilising three-dimensional image pattern matching is proposed which, in principle, is capable of unambiguous determination of the types and positions of atoms in small molecules from defocus series collected at only a…

Image and Video Processing · Electrical Eng. & Systems 2019-12-02 T. E. Gureyev , H. M. Quiney , A. Kozlov , L. J. Allen

Super-resolution imaging based on single molecule localization allows accessing nanometric-scale information in biological samples with high precision. However, complete measurements including molecule orientation are still challenging.…

Industrial use of ultrashort-pulse surface structuring would significantly increase by an effective utilization of the average laser powers available currently. However, the unexplained degradation of surfaces processed with numerous pulses…

Applied Physics · Physics 2022-01-21 F. Nyenhuis , P. N. Terekhin , T. Menold , B. Rethfeld , A. Michalowski , J. L'huillier

We report an ultrafast, parallel and beyond-the-master micro-patterning technique for ultrathin (30 nm-400 nm) non-absorbing polymer films by diffraction of a laser light through a two dimensional periodic aperture. The redistribution of…

Soft Condensed Matter · Physics 2011-01-28 Ankur Verma , Ashutosh Sharma , Giridhar U. Kulkarni

We present the experimental reconstruction of sub-wavelength features from the far-field intensity of sparse optical objects: sparsity-based sub-wavelength imaging combined with phase-retrieval. As examples, we demonstrate the recovery of…

Forescatter electron imaging is a popular microscopy technique, especially for scanning electron microscopes equipped with an electron backscatter diffraction detector. In principal, this method enables qualitative imaging of microstructure…

Materials Science · Physics 2018-06-04 Ben Britton , Daniel Goran , Vivian Tong

The advent of ultrafast pulsed X-ray free-electron lasers with very high brightness has enabled the determination of transient molecular structures of small and medium-sized organic molecules in excited states and undergoing chemical…

Chemical Physics · Physics 2025-10-07 Peter M. Weber , Brian Stankus , Adam Kirrander

Understanding the electron beam distribution in the longitudinal phase space (LPS) is crucial for free electron laser (FEL) facilities. Conventionally, LPS diagnostics utilize radio frequency (RF) deflecting structures to streak the…

Accelerator Physics · Physics 2026-05-28 Sergey Tomin , Igor Zagorodnov

Laser-induced electron diffraction is an evolving tabletop method, which aims to image ultrafast structural changes in gas-phase polyatomic molecules with sub-{\AA}ngstr\"om spatial and femtosecond temporal resolution. Here, we provide the…

It has been argued that in atomic-resolution transmission electron microscopy (TEM) of sparse weakly scattering structures, such as small biological molecules, multiple electron scattering usually has only a small effect, while the…

Optics · Physics 2021-11-16 T. E. Gureyev , H. M. Quiney , A. Kozlov , D. M. Paganin , G. Schmalz , L. J. Allen

Deploying 3D single-photon Lidar imaging in real world applications faces several challenges due to imaging in high noise environments and with sensors having limited resolution. This paper presents a deep learning algorithm based on…

Image and Video Processing · Electrical Eng. & Systems 2023-07-25 Abderrahim Halimi , Jakeoung Koo , Stephen McLaughlin

Electron backscatter diffraction is a widely used technique for nano- to micro-scale analysis of crystal structure and orientation. Backscatter patterns produced by an alloy solid solution matrix and its ordered superlattice exhibit only…

Materials Science · Physics 2020-10-13 Thomas P McAuliffe , David Dye , T Ben Britton
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