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Brillouin spectroscopy is a century-old technique that has recently received renewed interest, as modern instrumentation has transformed it into a powerful contactless and label-free probe of micromechanical properties for biomedical…
Starting from the hypothesis that the tubulin dimer is a conformationally bistable molecule - fluctuating between a curved and a straight configuration at room temperature - we develop a model for polymorphic dynamics of the microtubule…
Reconstituted cytoskeleton composites have emerged as a valuable model system for studying non equilibrium soft matter. Faithful capture of the dynamics of these three dimensional, dense networks calls for optical sectioning often…
Gravitational microlensing has become a mature technique for discovering small gravitational lenses in the Universe which are otherwise beyond our detection limits. Similarly, plasma microlensing can help us explore cosmic plasma lenses.…
The cellular cytoskeleton is an active material, driven out of equilibrium by molecular motor proteins. It is not understood how the collective behaviors of cytoskeletal networks emerge from the properties of the network's constituent motor…
A microscopic theory for the luminescence of ordered semiconductors is modified to describe photoluminescence of strongly disordered semiconductors. The approach includes both diagonal disorder and the many-body Coulomb interaction. As a…
The mitotic spindle lies at the heart of the spatio-temporal control over cellular components during cell division. The spindle consists of microtubules, which are not only crosslinked by motor proteins but also by passive binding proteins.…
The vast majority of the microscopy methods currently available to study biological samples require staining prior to imaging. Nevertheless, the ability to reveal specific cell structures or organelles in a label-free manner remains…
Imaging depth of optical microscopy has been fundamentally limited to millimeter or sub-millimeter due to light scattering. X-ray microscopy can resolve spatial details of few microns deeply inside a sample but the contrast resolution is…
In microtubule-based active nematics, motor-driven extensile motion of microtubule bundles powers chaotic large-scale dynamics. We quantify the interfilament sliding motion both in isolated bundles and in a dense active nematic. The…
The existing optical microscopes form an image by collecting photons emitted from an object. Here we report on the experimental realization of microscopy without the need for direct optical communication with the sample. To achieve this, we…
Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…
Size and shape are critical discriminators between molecular species and states. We describe a micro-chip based high-throughput imaging approach offering rapid and precise determination of molecular properties under native solution…
A novel non-invasive microscopy technique for imaging and sizing of folded DNA molecules with the use of photovoltaic tweezers and phase-sensitive detection is elaborated and realized. This novel method is compared with the state-of-the-art…
Biofilament-motor protein complexes are ubiquitous in biology and drive the transport of cargo vital for many fundamental cellular processes. As they move, motor proteins exert compressive forces on the filaments to which they are attached,…
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…
In this work, we present a method to characterise the transmission matrices of complex scattering media using a physics-informed, multi-plane neural network (MPNN) without the requirement of a known optical reference field. We use this…
Fluorescence microscopy has become a widely used tool for studying various biological structures of in vivo tissue or cells. However, quantitative analysis of these biological structures remains a challenge due to their complexity which is…
A microtubule (MT) is a hollow tube of approximately 25 nm diameter. The two ends of the tube are dissimilar and are designated as `plus' and `minus' ends. Motivated by the collective push and pull exerted by a bundle of MTs during…
By their very nature microscopy images of cells and tissues consist of a limited number of object types or components. In contrast to most natural scenes, the composition is known a priori. Decomposing biological images into semantically…