Related papers: Spatial fluorescence cross correlation spectroscop…
Fluorescence Correlation Spectroscopy (FCS) is a powerful single-molecule technique which allows for measuring motion (diffusion, flow), concentration, and molecular interaction kinetics of fluorescent molecules from picomolar to micromolar…
The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…
Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…
It has recently been proposed, with the help of numerical investigations, that fluorescence correlation spectroscopy at variable observation area can reveal the existence of a meshgrid of semi-permeable barriers hindering the…
Fluorescence Correlation Spectroscopy (FCS) is widely used to detect and quantify diffusion processes at the molecular level. The molecules of which diffusion is studied are marked with fluorescent dyes. It is commonly maintained that this…
A fluorescence correlation spectroscopy (FCS) system based on two independent measurement volumes is presented. The optical setup and data acquisition hardware are detailed, as well as a complete protocol to control the location, size and…
We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…
Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…
Accurate characterization using static light scattering (SLS) and dynamic light scattering (DLS) methods mandates the measurement and analysis of singly-scattered light. In turbid samples, the suppression of multiple scattering is therefore…
Spatial modulation microscopy is a technique originally developed for quantitative spectroscopy of individual nano-objects. Here, a parallel implementation of the spatial modulation microscopy technique is demonstrated based on a line…
We exploit memory effect speckle correlations for the imaging of incoherent linear (single-photon) fluorescent sources behind scattering tissue. While memory effect-based imaging techniques have been heavily studied in the past, for thick…
Using photonic devices, we developed a new approach to traditional spectroscopy where the spectral cross-correlation with a template spectrum can be done entirely on-device. By creating photonic devices with a carefully designed, modulated…
Spatial quantum correlations in the transverse degree of freedom promise to enhance optical resolution, image detection, and quantum communications through parallel quantum information encoding. In particular, the ability to observe these…
Fluorescence spectroscopy is an image correlation technique to analyze and characterize the molecular dynamics from a sequence of fluorescence images. Many image correlation techniques have been developed for different applications [1]. But…
A ubiquitous observation in crowded cell membranes is that molecular transport does not follow Fickian diffusion but exhibits subdiffusion. The microscopic origin of such a behaviour is not understood and highly debated. Here we discuss the…
Phase-only spatial light modulators (SLMs) are used in optical systems for several purposes. In this article, the main landmarks of SLM-based imaging systems are surveyed. In addition to conventional two-dimensional imaging, these systems…
We discuss the influence of the superconducting transition in a film on the fluorescence spectrum of a single molecule located nearby. We show that single molecule spectroscopy (SMS) should be an appropriate tool to detect the electric…
Spectral diffusion is a result of random spectral jumps of a narrow line as a result of a fluctuating environment. It is an important issue in spectroscopy, because the observed spectral broadening prevents access to the intrinsic line…
Spatially varying spectral modulation can be implemented using a liquid crystal spatial light modulator (SLM) since it provides an array of liquid crystal cells, each of which can be purposed to act as a programmable spectral filter array.…
The complete characterization of spatial coherence is difficult because the mutual coherence function is a complex-valued function of four independent variables. This difficulty limits the ability of controlling and optimizing spatial…