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Speckle-correlation imaging techniques are widely used for non-invasive imaging through complex scattering media. While light propagation through multimode fibers and scattering media share many analogies, reconstructing images through…

Accurate reconstruction of the spatial distributions of the Point Spread Function (PSF) is crucial for high precision cosmic shear measurements. Nevertheless, current methods are not good at recovering the PSF fluctuations of high spatial…

Cosmology and Nongalactic Astrophysics · Physics 2021-07-12 Tianhuan Lu , Jun Zhang , Fuyu Dong , Yingke Li , Dezi Liu , Liping Fu , Guoliang Li , Zuhui Fan

Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…

Biological Physics · Physics 2011-12-23 Charles Edouard Leroux , Irène Wang , Jacques Derouard , Antoine Delon

Optical diffraction tomography is an indispensable tool for studying objects in three-dimensions due to its ability to accurately reconstruct scattering objects. Until now this technique has been limited to coherent light because spatial…

Spatial light modulators are versatile devices employed in a vast range of applications to modify the transverse phase or amplitude profile of an incident light beam. Most experiments are designed to use a specific polarization which…

Quantum Physics · Physics 2014-05-06 G. Barreto Lemos , J. O. de Almeida , S. P. Walborn , P. H. Souto Ribeiro , M. Hor-Meyll

Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

The inherent non-linearity of intensity correlation functions can be used to spatially distinguish identical emitters beyond the diffraction limit, as achieved, for example, in Super-Resolution Optical Fluctuation Imaging (SOFI). Here, we…

Optics · Physics 2024-12-18 Yifan Chen , Chieh Tsao , Hendrik Utzat

Multifocal microscopy (MFM) offers high-speed three-dimensional imaging through the simultaneous image capture from multiple focal planes. Conventional MFM systems use a fabricated grating in the emission path for a single emission…

Biological Physics · Physics 2019-07-31 M. Junaid Amin , Sabine Petry , Haw Yang , Joshua W. Shaevitz

A new technique based on superposition of two speckle patterns is proposed and demonstrated for controlled modulation of the spatial polarization distribution of the resultant speckle. It is demonstrated both theoretically and…

Optics · Physics 2021-11-15 Abhijit Roy , Rakesh K. Singh , Maruthi M. Brundavanam

We present a variation of two-dimensional electronic spectroscopy that is capable of mapping spatially-varying differences in electronic couplings using a correlated map of excitation and detection frequencies, with sensitivity orders of…

We report broadband characterization of the propagation of light through a multiply scattering medium by means of its Multi-Spectral Transmission Matrix. Using a single spatial light modulator, our approach enables the full control of both…

The "spectral correlation function" analysis we introduce in this paper is a new tool for analyzing spectral-line data cubes. Our initial tests, carried out on a suite of observed and simulated data cubes, indicate that the spectral…

The detection and characterization of Earth-like exoplanets is one of the major science drivers for the next generation of telescopes. Current direct imaging instruments are limited by evolving non-common path aberrations (NCPAs). The NCPAs…

Instrumentation and Methods for Astrophysics · Physics 2022-03-14 Sebastiaan Y. Haffert

We introduce the basic elements of a spatio-angular theory of fluorescence microscopy, providing a unified framework for analyzing systems that image single fluorescent dipoles and ensembles of overlapping dipoles that label biological…

Several matrix approaches were developed to control light propagation through multiple scattering media under illumination of ultrashort pulses of light. These matrices can be recorded either with spectral or temporal resolution. Thanks to…

Optics · Physics 2018-06-08 Mickael Mounaix , Duc Minh Ta , Sylvain Gigan

It is known that a spatially partially coherent light field produces better imaging contrast compared to a spatially coherent field and that the contrast increases as the spatial coherence length of the field becomes smaller. The transverse…

Super-resolution optical fluctuation imaging (SOFI) provides a resolution beyond the diffraction limit by analysing stochastic fluorescence fluctuations with higher-order statistics. Using nth order spatio-temporal cross-cumulants the…

The present paper deals with an experimental feasibility study concerning the detection of long- range intermolecular interactions through molecular diffusion behavior in solution. This follows previous analyses, theoretical and numerical,…

The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion of membrane receptors and the kinetics of transcription factors governing the rates of gene expression. Different fluorescence…

Quantitative Methods · Quantitative Biology 2020-04-03 Maxime Woringer , Ignacio Izeddin , Cyril Favard , Hugues Berry

The degree of optical spatial coherence -a fundamental property of light that describes the mutual correlations between fluctuating electromagnetic fields- has proven challenging to control at the micrometer scale. Here we employ surface…

Optics · Physics 2016-12-30 Dongfang Li , Domenico Pacifici