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The spatial organization of enzymes often plays a crucial role in the functionality and efficiency of enzymatic pathways. To fully understand the design and operation of enzymatic pathways, it is therefore crucial to understand how the…

Subcellular Processes · Quantitative Biology 2015-06-17 Alexander Buchner , Filipe Tostevin , Florian Hinzpeter , Ulrich Gerland

Experiments show that the movement of eukaryotic cells is regulated by a process of phase separation of two competing enzymes on the cell membrane, that effectively amplifies shallow external gradients of chemical attractant. Notably, the…

Statistical Mechanics · Physics 2008-12-19 Teresa Ferraro , Antonio de Candia , Andrea Gamba , Antonio Coniglio

Enzymes within biochemical pathways are often colocalized, yet the consequences of specific spatial enzyme arrangements remain poorly understood. We study the impact of enzyme arrangement on reaction efficiency within a reaction-diffusion…

Molecular Networks · Quantitative Biology 2013-10-14 Alexander Buchner , Filipe Tostevin , Ulrich Gerland

Living systems contain intricate biochemical networks whose structure is closely related to their function and allows them to exhibit robust behavior in the presence of external stimuli. Such networks typically involve catalytic enzymes,…

Biological Physics · Physics 2025-10-27 Vincent Ouazan-Reboul , Ramin Golestanian , Jaime Agudo-Canalejo

Enzyme-enriched condensates can organize the spatial distribution of their substrates by catalyzing non-equilibrium reactions. Conversely, an inhomogeneous substrate distribution induces enzyme fluxes through substrate-enzyme interactions.…

Biological Physics · Physics 2023-03-29 Leonardo Demarchi , Andriy Goychuk , Ivan Maryshev , Erwin Frey

Multisite covalent modification of proteins is omnipresent in eukaryotic cells. A well-known example is the mitogen-activated protein kinase (MAPK) cascade, where in each layer of the cascade a protein is phosphorylated at two sites. It has…

Molecular Networks · Quantitative Biology 2015-05-13 Koichi Takahashi , Sorin Tanase-Nicola , Pieter Rein ten Wolde

In molecular communication, the heavy tail nature of molecular signals causes inter-symbol interference (ISI). Because of this, it is difficult to decrease symbol periods and achieve high data rate. As a probable solution for ISI…

Emerging Technologies · Computer Science 2017-03-21 Yae Jee Cho , H. Birkan Yilmaz , Weisi Guo , Chan-Byoung Chae

Diffusive motion of regulatory enzymes on biopolymers with eventual capture at a reaction site is a common feature in cell biology. Using a lattice gas model we study the impact of diffusion and capture for a microtubule polymerase and a…

Biological Physics · Physics 2016-08-12 Emanuel Reithmann , Louis Reese , Erwin Frey

A living cell senses its environment and responds to external signals. In this work, we study theoretically, the precision at which cells can determine the position of a spatially localized transient extracellular signal. To this end, we…

Cell Behavior · Quantitative Biology 2019-09-04 Vaibhav H. Wasnik , Peter Lipp , Karsten Kruse

Spatial heterogeneity is a hallmark of living systems, even at the molecular scale in individual cells. A key example is the partitioning of membrane-bound proteins via lipid domain formation or cytoskeleton-induced corralling. Yet the…

Molecular Networks · Quantitative Biology 2013-11-19 Andrew Mugler , Filipe Tostevin , Pieter Rein ten Wolde

The propagation of signalling molecules within cellular networks is affected by network topology, but also by the spatial arrangement of cells in the networks. Understanding the collective reaction--diffusion behaviour in space of signals…

Disordered Systems and Neural Networks · Physics 2025-01-09 Adel Mehrpooya , Vivien J. Challis , Pascal R. Buenzli

Enzymes have been recently proposed to have mechanical activity associated with their chemical activity. In a number of recent studies, it has been reported that enzymes undergo enhanced diffusion in the presence of their corresponding…

Biological Physics · Physics 2021-04-07 Jaime Agudo-Canalejo , Tunrayo Adeleke-Larodo , Pierre Illien , Ramin Golestanian

It is now well established that the cell is a highly crowded environment. Yet, the effects of crowding on the dynamics of signaling pathways, gene regulation networks and metabolic networks are still largely unknown. Crowding can alter both…

Molecular Networks · Quantitative Biology 2013-11-19 Pieter Rein ten Wolde , Andrew Mugler

Electrical signals in excitable cells involve spatially localized ionic fluxes through ion channels and pumps on cellular lipid membranes. Common approaches to understand how these localized fluxes spread assume that the membrane and the…

Soft Condensed Matter · Physics 2024-07-17 Hyeongjoo Row , Joshua B. Fernandes , Kranthi K. Mandadapu , Karthik Shekhar

A protein undergoes conformational dynamics with multiple time scales, which results in fluctuating enzyme activities. Recent studies in single molecule enzymology have observe this "age-old" dynamic disorder phenomenon directly. However,…

Biomolecules · Quantitative Biology 2009-11-10 Zhanghan Wu , Vlad Elgart , Hong Qian , Jianhua Xing

During the past decade, with the significant progress of computational power as well as ever-rising data availability, deep learning techniques became increasingly popular due to their excellent performance on computer vision problems. The…

Quantitative Methods · Quantitative Biology 2017-07-20 Afshine Amidi , Shervine Amidi , Dimitrios Vlachakis , Vasileios Megalooikonomou , Nikos Paragios , Evangelia I. Zacharaki

Several different enzymes display an apparent diffusion coefficient that increases with the concentration of their substrate. Moreover, their motion becomes directed in substrate gradients. Currently, there are several competing models for…

Biological Physics · Physics 2020-09-23 Giovanni Giunta , Hamid Seyed-Allaei , Ulrich Gerland

We examine the stochastic dynamics of two enzymes that are mechanically coupled to each other, e.g., through an elastic substrate or a fluid medium. The enzymes undergo conformational changes during their catalytic cycle, which itself is…

Statistical Mechanics · Physics 2021-11-16 Jaime Agudo-Canalejo , Tunrayo Adeleke-Larodo , Pierre Illien , Ramin Golestanian

Many enzymes appear to diffuse faster in the presence of substrate and to drift either up or down a concentration gradient of their substrate. Observations of these phenomena, termed enhanced enzyme diffusion (EED) and enzyme chemotaxis,…

Chemical Physics · Physics 2019-10-29 Mudong Feng , Michael K. Gilson

We investigate the problem of signal transduction via a descriptive analysis of the spatial organization of the complement of proteins exerting a certain function within a cellular compartment. We propose a scheme to assign a numerical…

Molecular Networks · Quantitative Biology 2012-02-03 Federico Felizzi , Jerome Galtier , Georgios Fengos , Dagmar Iber
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