English

Fast fluorescence dynamics in non-ratiometric calcium indicators

Biological Physics 2009-03-25 v2 Soft Condensed Matter
Authors: M. Gersbach , D. L. Boiko , C. Niclass , C. Petersen , E. Charbon
View on arXiv ↗ PDF ↗ DOI ↗

Abstract

A fluorescence decay of high-affinity non-ratiometric Ca2+ indicator Oregon Green BAPTA-1 (OGB-1) is analyzed with unprecedented temporal resolution in the two-photon excitation regime. A triple exponential decay is shown to best fit the fluorescence dynamics of OGB-1. We provide a new model for accurate measurements of the free Ca2+ concentration and dissociation constants of non-ratiometric calcium indicators.

Cite

@article{arxiv.0807.2756,
  title  = {Fast fluorescence dynamics in non-ratiometric calcium indicators},
  author = {M. Gersbach and D. L. Boiko and C. Niclass and C. Petersen and E. Charbon},
  journal= {arXiv preprint arXiv:0807.2756},
  year   = {2009}
}

Comments

3 pages, 2 figures, figures revised, added chi-square goodness of fit

Related papers

View all related →
Soft Condensed Matter · Physics
A Solid-State System for High Temporal Resolution Fluorescence Lifetime Measurements
M. Gersbach, D. L. Boiko, C. Niclass, C. Petersen +1
2008-03-24
Materials Science · Physics
Measurement of the beta parameter of activated CaCO3 using time-resolved luminescence spectroscopy
Stephen Tapsak, Brielle Hunt, J. H. Huckans
2023-01-10
Materials Science · Physics
Blue luminescence of SrTiO3 under intense optical excitation
A. Rubano, D. Paparo, F. Miletto, U. Scotti di Uccio +1
2009-11-24
Biological Physics · Physics
In situ fiber-optical monitoring of cytosolic calcium in tissue explant cultures
Manuel Ryser, Lisa Künzi, Marianne Geiser, Martin Frenz +1
2014-12-12
Strongly Correlated Electrons · Physics
Decay and coherence of two-photon excited yellow ortho-excitons in Cu2O
K. Karpinska, M. Mostovoy, M. A. van der Vegte, A. Revcolevschi +1
2007-05-23
Optics · Physics
Optical properties of bismuth-doped SiO2- or GeO2-based glass core optical fibers
Elena G. Firstova
2015-03-18
Instrumentation and Detectors · Physics
Signal identification with Kalman Filter towards background-free neutrinoless double beta decay searches in gaseous detectors
Tao Li, Shaobo Wang, Yu Chen, Ke Han +5
2021-07-07
Strongly Correlated Electrons · Physics
Ultrafast charge-transfer dynamics in Ca$_2$CuO$_2$Cl$_2$ from time-resolved optical reflectivity
Haiyun Huang, Xiu Zhang, Junzhi Zhu, Jianfa Zhao +6
2025-05-23
Biological Physics · Physics
High Spatio-Temporal-Resolution Detection of Chlorophyll Fluorescence Dynamics from a Single Chloroplast with Confocal Imaging Fluorometer
Yi-Chin Tseng, Shi-Wei Chu
2016-11-10
Soft Condensed Matter · Physics
Nonequilibrium Dynamics of Optical Lattice-Loaded BEC Atoms: Beyond HFB Approximation
Ana Maria Rey, B. L. Hu, Esteban Calzetta, Albert Roura +1
2013-05-29
Computational Engineering, Finance, and Science · Computer Science
Mapping the spatiotemporal dynamics of calcium signaling in cellular neural networks using optical flow
Marius Buibas, Diana Yu, Krystal Nizar, Gabriel A. Silva
2010-01-22
Quantum Physics · Physics
Non-hermitian exciton dynamics in a photosynthetic unit system
A. Thilagam
2012-04-03
Materials Science · Physics
Ce3+:CaSc2O4 Crystal Fibers for Green Light Emission: Growth Issues and Characterization
Detlef Klimm, Jan Philippen, Toni Markurt, Albert Kwasniewski
2014-07-16
Biological Physics · Physics
Widefield two-photon excitation without scanning: live cell microscopy with high time resolution and low photo-bleaching
Rumelo Amor, Johanna Trägårdh, Gillian Robb, Louise Wilson +5
2015-01-05
Biological Physics · Physics
The coherent dynamics of photoexcited green fluorescent proteins
Riccardo A. G. Cinelli, Valentina Tozzini, Vittorio Pellegrini, Fabio Beltram +5
2009-11-07
Materials Science · Physics
Transient photoluminescence enhancement as a probe of the structure of impurity-trapped excitons in CaF$_2$:Yb$^{2+}$
Michael F. Reid, Pubudu S. Senanayake, Jon-Paul R. Wells, Giel Berden +4
2011-10-05
Superconductivity · Physics
Photoinduced Changes of Reflectivity in Single Crystals of YBa2Cu3O6.5 (Ortho II)
G. P. Segre, N. Gedik, J. Orenstein, D. A. Bonn +2
2009-11-07
Strongly Correlated Electrons · Physics
Nonequilibrium dynamics of $\alpha$-RuCl$_{3}$ -- a time-resolved magneto-optical spectroscopy study
Julian Wagner, Anuja Sahasrabudhe, Rolf Versteeg, Zhe Wang +4
2022-10-12
Atomic Physics · Physics
Offset Simultaneous Conjugate Atom Interferometers
Weicheng Zhong, Richard H. Parker, Zachary Pagel, Chenghui Yu +1
2020-05-20
Mesoscale and Nanoscale Physics · Physics
Charge separation in donor-C60 complexes with real-time Green's functions: The importance of nonlocal correlations
E. Viñas Boström, A. Mikkelsen, C. Verdozzi, E. Perfetto +1
2018-01-25
Materials Science · Physics
Analysis of the structural characteristics and optoelectronic properties of CaTiO3 as a non-toxic raw material for solar cells: a DFT study
D. D. Nematov, A. S. Burhonzoda, F. Shokir
2022-11-01
High Energy Astrophysical Phenomena · Physics
Methods for statistical detection of GRBs in the context of the LST-CTAO
Léo Le Moigne, Mathieu de Bony de Lavergne, Armand Fiasson, Edna Ruiz-Velasco +2
2025-09-23
High Energy Physics - Phenomenology · Physics
Feasibility study for a model independent measurement of 2$\beta + \gamma$ in $B^0$ decays using $D^- K^0 \pi^+$ final states
F. Polci, M. -H. Schune, A. Stocchi
2007-05-23
Materials Science · Physics
Excited State Spectroscopy of Boron Vacancy Defects in Hexagonal Boron Nitride using Time-Resolved Optically Detected Magnetic Resonance
Simon Baber, Ralph N. E. Malein, Prince Khatri, Paul S. Keatley +4
2022-01-24
Instrumentation and Detectors · Physics
Towards a background-free neutrinoless double beta decay experiment based on a fluorescent bicolor sensor
Iván Rivilla, Borja Aparicio, Juan M. Bueno, David Casanova +12
2019-09-17
R2 v1 2026-06-21T11:01:39.307Z