English

High Throughput Screening of Expression Constructs using Microcapillary Arrays

Quantitative Methods 2025-01-30 v1 Biomolecules

Abstract

Gene expression is a complex phenomenon involving numerous interlinked variables, and studying these variables to control expression is essential in bioengineering and biomanufacturing. While cloning techniques for achieving plasmid libraries that cover large design spaces exist, multiplex techniques offering cell culture screening at similar scales are still lacking. We introduced a microcapillary array-based platform aimed at high-throughput, multiplex screening of miniature cell cultures through fluorescent reporters.

Keywords

Cite

@article{arxiv.2501.17647,
  title  = {High Throughput Screening of Expression Constructs using Microcapillary Arrays},
  author = {Khushank Singhal and Harry Adamson and Thomas Baer and Howard Salis and Melik Demirel},
  journal= {arXiv preprint arXiv:2501.17647},
  year   = {2025}
}

Comments

30 pages, 6 figures

R2 v1 2026-06-28T21:23:48.424Z