Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking
Abstract
Forward-viewing endoscopic optical coherence tomography (OCT) provides 3D imaging in vivo, and can be combined with widefield fluorescence imaging by use of a double-clad fiber. However, it is technically challenging to build a high-performance miniaturized 2D scanning system with a large field-of-view. In this paper we demonstrate how a 1D scanning probe, which produces cross-sectional OCT images (B-scans) and 1D fluorescence T-scans, can be transformed into a 2D scanning probe by manual scanning along the second axis. OCT volumes are assembled from the B-scans using speckle decorrelation measurements to estimate the out-of-plane motion along the manual scan direction. Motion within the plane of the B-scans is corrected using image registration by normalized cross correlation. En-face OCT slices and fluorescence images, corrected for probe motion in 3D, can be displayed in real-time during the scan. For a B-scan frame rate of 250 Hz, and an OCT lateral resolution of approximately 20 micrometers, the approach can handle out-of-plane motion at speeds of up to 4 mm/s.
Cite
@article{arxiv.2110.09450,
title = {Endoscopic en-face optical coherence tomography and fluorescence imaging using correlation-based probe tracking},
author = {Manuel J. Marques and Michael R. Hughes and Adrián F. Uceda and Grigory Gelikonov and Adrian Bradu and Adrian Podoleanu},
journal= {arXiv preprint arXiv:2110.09450},
year = {2021}
}
Comments
16 pages