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Systems biology approaches combining theoretical modeling with experiments have been singularly successful in uncovering novel features of cellular phenomena. One such feature is that of binary gene expression in which the expression level…

Subcellular Processes · Quantitative Biology 2012-07-31 Indrani Bose

MicroRNA-mediated regulation of gene expression is characterised by some distinctive features that set it apart from unregulated and transcription factor-regulated gene expression. Recently, a mathematical model has been proposed to…

Quantitative Methods · Quantitative Biology 2012-10-19 Indrani Bose , Sayantari Ghosh

A single gene, regulating its own expression via a positive feedback loop, constitutes a common motif in gene regulatory networks and signalling cascades. Recent experiments on the development of competence in the bacterial population…

Quantitative Methods · Quantitative Biology 2007-05-23 Rajesh Karmakar , Indrani Bose

Recent experiments at the level of a single cell have shown that gene expression occurs in abrupt stochastic bursts. Further, in an ensemble of cells, the levels of proteins produced have a bimodal distribution. In a large fraction of…

Soft Condensed Matter · Physics 2009-11-07 Siddhartha Roy , Indrani Bose , Subhrangshu Sekhar Manna

Inside individual cells, expression of genes is stochastic across organisms ranging from bacterial to human cells. A ubiquitous feature of stochastic expression is burst-like synthesis of gene products, which drives considerable…

Molecular Networks · Quantitative Biology 2016-09-13 Pavol Bokes , Abhyudai Singh

Gene expression (GE) is an inherently random or stochastic or noisy process. The randomness in different steps of GE, e.g., transcription, translation, degradation, etc., leading to cell-to-cell variations in mRNA and protein levels. This…

Molecular Networks · Quantitative Biology 2021-01-12 Rajesh Karmakar , Amit Kumar Das

Gene expression is inherently noisy as many steps in the read-out of the genetic information are stochastic. To disentangle the effect of different sources of stochasticity in such systems, we consider various models that describe some…

Molecular Networks · Quantitative Biology 2015-06-05 Rahul Marathe , David Gomez , Stefan Klumpp

We study the response of an autoregulated gene to a range of concentrations of signal molecules. We show that transcriptional leakage and noise due to translational bursting have the opposite effects. In a positively autoregulated gene,…

Subcellular Processes · Quantitative Biology 2015-01-19 Anna Ochab-Marcinek , Marcin Tabaka

Appropriate regulation of gene expression is essential to ensure that protein synthesis occurs in a selective manner. The control of transcription is the most dominant type of regulation mediated by a complex of molecules such as…

Molecular Networks · Quantitative Biology 2010-02-04 Rajesh Karmakar

Genetic switch systems with mutual repression of two transcription factors are studied using deterministic methods (rate equations) and stochastic methods (the master equation and Monte Carlo simulations). These systems exhibit bistability,…

Molecular Networks · Quantitative Biology 2007-05-23 Adiel Loinger , Azi Lipshtat , Nathalie Q. Balaban , Ofer Biham

In this paper we develop a model of stochastic gene expression, which is an extension of the model investigated in the paper [T. Lipniacki, P. Paszek, A. Marciniak-Czochra, A.R. Brasier, M. Kimmel, Transcriptional stochasticity in gene…

Probability · Mathematics 2015-10-20 Ryszard Rudnicki , Andrzej Tomski

Stochasticity in gene expression can give rise to fluctuations in protein levels and lead to phenotypic variation across a population of genetically identical cells. Recent experiments indicate that bursting and feedback mechanisms play…

Molecular Networks · Quantitative Biology 2015-06-22 Niraj Kumar , Thierry Platini , Rahul V. Kulkarni

The intrinsic stochasticity of gene expression can lead to large variability in protein levels for genetically identical cells. Such variability in protein levels can arise from infrequent synthesis of mRNAs which in turn give rise to…

Biological Physics · Physics 2015-05-27 Vlad Elgart , Tao Jia , Andrew T. Fenley , Rahul V. Kulkarni

Stochasticity in gene expression can result in fluctuations in gene product levels. Recent experiments indicated that feedback regulation plays an important role in controlling the noise in gene expression. A quantitative understanding of…

Molecular Networks · Quantitative Biology 2019-12-11 Zihao Wang , Zhenquan Zhang , Tianshou Zhou

Gene expression has a stochastic component owing to the single molecule nature of the gene and the small number of copies of individual DNA binding proteins in the cell. We show how the statistics of such systems can be mapped on to quantum…

Disordered Systems and Neural Networks · Physics 2009-11-10 Masaki Sasai , Peter G. Wolynes

In this paper, we consider two stochastic models of gene expression in prokaryotic cells. In the first model, sixteen biochemical reactions involved in transcription, translation and transcriptional regulation in the presence of inducer…

Condensed Matter · Physics 2007-05-23 Indrani Bose , Rajesh Karmakar , Siddhartha Roy

The expression of genes usually follows a two-step procedure. First, a gene (encoded in the genome) is transcribed resulting in a strand of (messenger) RNA. Afterwards, the RNA is translated into protein. Classically, this gene expression…

Molecular Networks · Quantitative Biology 2014-08-08 Martin Jansen , Peter Pfaffelhuber

Cell growth and gene expression, essential elements of all living systems, have long been the focus of biophysical interrogation. Advances in single-cell methods have invigorated theoretical studies into these processes. However, until…

Subcellular Processes · Quantitative Biology 2023-11-22 Ido Golding , Ariel Amir

Stochastic kinetic models of genetic expression are able to describe protein fluctuations. A comparative study of the canonical and a feedback model is given here by using stochastic simulation methods. The feedback model is skeleton model…

Molecular Networks · Quantitative Biology 2018-09-06 Raoul R. Wadhwa , Laszlo Zalanyi , Judit Szente , Laszlo Negyessy , Peter Erdi

In this article we demonstrate that the so-called bursting production of molecular species during gene expression may be an artifact caused by low time resolution in experimental data collection and not an actual burst in production. We…

Probability · Mathematics 2011-12-15 Romain Yvinec , Alexandre F. Ramos
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