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We propose a stochastic Gillespie scheme to describe the temporal fluctuations of local denaturation zones in double-stranded DNA as a single molecule time series. It is demonstrated that the model recovers the equilibrium properties. We…

Biomolecules · Quantitative Biology 2009-11-11 Suman Kumar Banik , Tobias Ambjornsson , Ralf Metzler

As sections of a strand duplexed DNA denature when exposed to high temperature, the excess linking number is taken up by the undenatured portions of the molecule. The mechanical energy that arises because of the overwinding of the…

Soft Condensed Matter · Physics 2009-11-07 Joseph Rudnick , Robijn Bruinsma

Nucleic acids are highly deformable helical molecules constantly stretched, twisted and bent in their biological functioning. Single molecule experiments have shown that double stranded (ds)-RNA and standard ds-DNA have opposite…

Soft Condensed Matter · Physics 2023-06-28 Marco Zoli

We present a general framework to study the thermodynamic denaturation of double-stranded DNA under superhelical stress. We report calculations of position- and size-dependent opening probabilities for bubbles along the sequence. Our…

Biomolecules · Quantitative Biology 2013-05-29 Daniel Jost , Asif Zubair , Ralf Everaers

DNA flexibility is a key determinant of biological function, from nucleosome positioning to transcriptional regulation, motivating a direct measurement of the bend-torque response of individual DNA molecules. In this work, DNA bending is…

DNA loop formation is one of several mechanisms used by organisms to regulate genes. The free energy of forming a loop is an important factor in determining whether the associated gene is switched on or off. In this paper we use an elastic…

Biomolecules · Quantitative Biology 2015-06-26 Prashant K. Purohit , Philip C. Nelson

It is well known that transcription can induce torsional stress in DNA, affecting the activity of nearby genes or even inducing structural transitions in the DNA duplex. It has long been assumed that the generation of significant torsional…

Soft Condensed Matter · Physics 2009-10-31 Philip Nelson

Cells deploy robust mechanisms to repair DNA damage, safeguarding genomic stability and cellular health, but the physical principles underlying these processes remain incompletely understood. Experiments show \emph{in vitro} that upon a DNA…

Biological Physics · Physics 2026-05-26 Rakesh Das , Tarun Mascarenhas , Nagaraja Chappidi , Simon Alberti , Frank Jülicher

Gene transcription by a RNA Polymerase (RNAP) enzyme requires that double-stranded DNA be locally and transiently opened, which results in an increase of DNA supercoiling downstream of the RNAP and a decrease of supercoiling upstream of it.…

Biological Physics · Physics 2024-10-15 Marc Joyeux

Minuscule molecular forces can transform DNA into a structure that is elongated by more than half its original length. We demonstrate that this pronounced conformational transition is of relevance to ongoing experimental and theoretical…

Materials Science · Physics 2010-07-06 Paul Maragakis , Ryan Lee Barnett , Efthimios Kaxiras , Marcus Elstner , Thomas Frauenheim

The experiment that the high spin selectivity and the length-dependent spin polarization are observed in double-stranded DNA [Science ${\bf 331}$, 894 (2011)], is elucidated by considering the combination of the spin-orbit coupling, the…

Mesoscale and Nanoscale Physics · Physics 2015-06-03 Ai-Min Guo , Qing-feng Sun

We study the effects of the shear force on the rupture mechanism on a double stranded DNA. Motivated by recent experiments, we perform the atomistic simulations with explicit solvent to obtain the distributions of extension in hydrogen and…

Soft Condensed Matter · Physics 2015-06-17 S. Nath , T. Modi , R. K. Mishra , D. Giri , B. P. Mandal , S. Kumar

We discuss the effects of external stress on the thermal denaturation of homogeneous DNA. Pulling double-stranded DNA at each end exerts a profound effect on the thermal denaturation, or melting, of a long segment of this molecule. We…

Soft Condensed Matter · Physics 2009-11-13 Joseph Rudnick , Tatiana Kuriabova

The force induced unzipping transition of a double stranded DNA is considered from a purely thermodynamic point of view. This analysis provides us with a set of relations that can be used to test microscopic theories and experiments. The…

Soft Condensed Matter · Physics 2015-06-18 Poulomi Sadhukhan , Somendra M. Bhattacharjee

We predict a novel temperature-driven phase transition of DNA below the melting transition. The additional, intermediate phase exists for repetitive sequences, when the two strands have different lengths. In this phase, the excess bases of…

Biomolecules · Quantitative Biology 2007-05-23 Richard A. Neher , Ulrich Gerland

We propose an Ashkin-Teller like model for elastic response of DNA molecule to external force and torque. The base-stacking interaction is described in a simple and uniform way. We obtain the phase diagram of dsDNA, and in particular, the…

Biological Physics · Physics 2015-05-13 Zhe Chang , Ping Wang , Ying-Hong Zheng

We study numerically a disordered version of the model for DNA denaturation transition (DSAW-DNA) consisting of two interacting SAWs in 3d, which undergoes a first order transition in the homogeneous case. The two possible values eAT and…

Soft Condensed Matter · Physics 2009-11-11 Barbara Coluzzi

We present a simple model which describes elastic response of single-stranded DNA (ssDNA) to stretching, including the regime of very high force (up to 1000 pN). ssDNA is modelled as a discreet persistent chain, whose ground state is a…

Biomolecules · Quantitative Biology 2009-11-13 Alexei V. Tkachenko

When the DNA double helix is subjected to external forces it can stretch elastically to elongations reaching 100% of its natural length. These distortions, imposed at the mesoscopic or macroscopic scales, have a dramatic effect on…

Materials Science · Physics 2009-11-13 Ryan Barnett , Paul Maragakis , Ari Turner , Maria Fyta , Efthimios Kaxiras

Recent experiments showed that multiple copies of the molecular machine RNA polymerase (RNAP) can efficiently synthesize mRNA collectively in the active state of the promoter. However, environmentally-induced promoter repression results in…

Subcellular Processes · Quantitative Biology 2021-12-01 Purba Chatterjee , Nigel Goldenfeld , Sangjin Kim