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We propose a minimal model for spherical proteins with aeolotopic pair interactions to describe the equilibrium phase behavior of lysozyme. The repulsive screened Coulomb interactions between the particles are taken into account assuming…

Soft Condensed Matter · Physics 2012-03-27 Christoph Gogelein , Gerhard Nagele , Remco Tuinier , Thomas Gibaud , Anna Stradner , Peter Schurtenberger

A simple model of globular proteins which incorporates anisotropic attractions is proposed. It is closely related to models used to model simple hydrogen-bonding molecules such as water. Theories for both the fluid and solid phases are…

Soft Condensed Matter · Physics 2009-10-31 Richard P. Sear

We present a combined experimental and numerical study of the equilibrium cluster formation in globular protein solutions under no-added salt conditions. We show that a cluster phase emerges as a result of a competition between a long-range…

The effect of molecule size (excluded volume) and the range of interaction on the surface tension, phase diagram and nucleation properties of a model globular protein is investigated using a combinations of Monte Carlo simulations and…

Statistical Mechanics · Physics 2015-03-13 Patrick Grosfils , James F. Lutsko

We have studied a model of a complex fluid consisting of particles interacting through a hard core and a short range attractive potential of both Yukawa and square-well form. Using a hybrid method, including a self-consistent and quite…

Intermolecular interactions in protein solutions in general contain many contributions. If short-range attractions dominate, the state diagram exhibits liquid-liquid phase separation (LLPS) that is metastable with respect to…

The dilute fluid phase of model globular proteins is studied. The model possesses a fluid-fluid transition buried within the fluid-crystal coexistence region, as do some globular proteins. If this fluid-fluid transition is not buried deep…

Soft Condensed Matter · Physics 2007-05-23 Richard P. Sear

We report a numerical simulation for the phase diagram of a simple two dimensional model, similar to one proposed by Noro and Frenkel [J. Chem. Phys. \textbf{114}, 2477 (2001)] for membrane proteins, but one that includes the role of the…

Soft Condensed Matter · Physics 2009-11-11 D. L. Pagan , A. Shiryayev , T. P. Connor , J. D. Gunton

In this work, an improved methodology for studying interactions of proteins in solution by small-angle scattering, is presented. Unlike the most common approach, where the protein-protein correlation functions $g_{ij}(r)$ are approximated…

Statistical Mechanics · Physics 2009-11-07 Francesco Spinozzi , Domenico Gazzillo , Achille Giacometti , Paolo Mariani , Flavio Carsughi

Comments: 6 pages RevTeX, 6 Postscript figures. We review a statistical mechanics treatment of the stability of globular proteins based on a simple model Hamiltonian taking into account protein self interactions and protein-water…

Condensed Matter · Physics 2009-10-31 Alex Hansen , Mogens H. Jensen , Kim Sneppen , Giovanni Zocchi

We investigate theoretically the fluid-crystal coexistence of solutions of globular charged nanoparticles like proteins and inorganic colloids. The thermodynamic properties of the fluid phase are computed via the optimized Baxter model.…

Soft Condensed Matter · Physics 2009-11-11 Peter Prinsen , Theo Odijk

Grand canonical Monte Carlo simulations are used to explore the metastable fluid-fluid coexistence curve of the modified Lennard-Jones model of globular proteins of ten Wolde and Frenkel (Science, v277, 1975 (1997)). Using both mixed-field…

Soft Condensed Matter · Physics 2009-11-10 D. L. Pagan , M. E. Gracheva , J. D. Gunton

The main goal of this paper is to assess the limits of validity, in the regime of low concentration and strong Coulomb coupling (high molecular charges), for a simple perturbative approximation to the radial distribution functions (RDF),…

Statistical Mechanics · Physics 2009-11-11 Achille Giacometti , Domenico Gazzillo , Giorgio Pastore , T. Kanti Das

Liquid-liquid phase separation (LLPS) of protein solutions is governed by highly complex protein-protein interactions. Nevertheless, it has been suggested that based on the extended law of corresponding states (ELCS), as proposed for…

Soft Condensed Matter · Physics 2023-02-08 Jan Hansen , Stefan U. Egelhaaf , Florian Platten

In view of the notorious complexity of protein--protein interactions, simplified models of proteins treated as patchy particles offer a promising strategy to obtain insight into the mechanism of crystallization. Here we report…

Soft Condensed Matter · Physics 2019-10-16 Jens Glaser , Sharon C. Glotzer

The interplay of phase separation and dynamical arrest can lead to the formation of gels and glasses, which is relevant for such diverse fields as hard and soft condensed matter physics, materials science, food engineering and…

We studied the crystallization of a globular protein, lysozyme, in the cubic phase of the lipid monoolein. The solubility of lysozyme in salt solution decreased by a factor of $\sim 4$ when confined in cubic phase. Calculations and Monte…

Soft Condensed Matter · Physics 2007-05-23 S. Tanaka , S. U. Egelhaaf , W. C. K. Poon

Advances in experimental techniques and in theoretical models have improved our understanding of protein crystallization. But they have also left open questions regarding the protein phase behavior and self-assembly kinetics, such as why…

Soft Condensed Matter · Physics 2014-03-19 Diana Fusco , Patrick Charbonneau

A simulation model is presented, comprising elastic spheres with a short range attraction. Besides conservative forces, radial- and shear friction, and radial noise are added. The model can be used to simulate colloids, granular solids and…

Soft Condensed Matter · Physics 2008-11-19 Robert D. Groot , Simeon D. Stoyanov

Studying protein interactions at low temperatures has important implications for optimizing cryostorage processes of biological tissue, food, and protein-based drugs. One of the major challenges is related to the formation of ice…

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