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We study theoretically the unzipping of a double stranded DNA from a condensed globule state by an external force. At constant force, we find that the double stranded DNA unzips an at critical force Fc and the number of unzipped monomers M…

Soft Condensed Matter · Physics 2007-05-23 Pui-Man Lam , J. C. Levy

The possibility of detecting mutations in a DNA from force measurements (as a first step towards sequence analysis) is discussed theoretically based on exact calculations. The force signal is associated with the domain wall separating the…

Statistical Mechanics · Physics 2009-11-07 Somendra M. Bhattacharjee , D. Marenduzzo

The histone-DNA interaction in the nucleosome is a fundamental mechanism of genomic compaction and regulation, which remains largely unkown despite a growing structural knowledge of the complex. Here, we propose a framework for the…

Biological Physics · Physics 2015-06-23 Sam Meyer , Ralf Everaers

Nucleosomes form the basic unit of compaction within eukaryotic genomes and their locations represent an important, yet poorly understood, mechanism of genetic regulation. Quantifying the strength of interactions within the nucleosome is a…

Biological Physics · Physics 2016-08-25 Joshua Lequieu , Andres Cordoba , David C. Schwartz , Juan J. de Pablo

We investigate the emergence of sustained spatio-temporal behaviors in reaction-phase separation systems. We focus on binary systems, in which either one or both species can phase separate, and we discuss the stability of the homogeneous…

Pattern Formation and Solitons · Physics 2024-08-08 Dino Osmanovic , Elisa Franco

The double-helical structure of DNA results from canonical base pairing and stacking interactions. However, variations from steady-state conformations result from mechanical perturbations in cells. These different topologies have…

Biomolecules · Quantitative Biology 2019-04-05 Jack W Shepherd , Robert J Greenall , Matt I J Probert , Agnes Noy , Mark C Leake

The sequence-dependent structural variability and conformational dynamics of DNA play pivotal roles in many biological milieus, such as in the site-specific binding of transcription factors to target regulatory elements. To better…

Biomolecules · Quantitative Biology 2014-07-22 Cameron Mura , J. Andrew McCammon

We propose a combined experimental (Atomic Force Microscopy) and theoretical study of the structural and dynamical properties of nucleosomes. In contrast to biochemical approaches, this method allows to determine simultaneously the DNA…

Test experiments of hybridization in DNA microarrays show systematic deviations from the equilibrium isotherms. We argue that these deviations are due to the presence of a partially hybridized long-lived state, which we include in a kinetic…

Biomolecules · Quantitative Biology 2010-06-16 J. Hooyberghs , M. Baiesi , A. Ferrantini , E. Carlon

Intracellular positive ions neutralise negative charges on the phosphates of a DNA strand conferring greater strength on the hydrogen bonds that connect complementary strands into a double helix and so confer enhanced stability. Beyond a…

Soft Condensed Matter · Physics 2016-04-25 Arghya Maity , Amar Singh , Navin Singh

We use X-ray scattering and molecular simulations to investigate the structural properties of complexes of multivalent cationic lipids and DNA molecules. At low mole fraction of neutral lipids (NLs), $\Phi_{\rm NL}$, the complexes show…

Soft Condensed Matter · Physics 2016-08-14 Oded Farago , Kai Ewert , Ayesha Ahmad , Heather M. Evans , Niels Grønbech-Jensen , Cyrus R. Safinya

Genetic stability is a key factor in maintaining, survival and reproduction of biological cells. It relies on many processes, but one of the most important is a {\it homologous recombination}, in which the repair of breaks in…

Subcellular Processes · Quantitative Biology 2017-04-05 M. P. Kochugaeva , A. A. Shvets , A. B. Kolomeisky

Base stacking is crucial in nucleic acid stabilization, from DNA duplex hybridization to single-stranded DNA (ssDNA) protein binding. While stacking energies are tiny in ssDNA, they are inextricably mixed with hydrogen bonding in DNA base…

Soft Condensed Matter · Physics 2024-11-19 Xavier Viader-Godoy , Maria Manosas , Felix Ritort

The flexibility of short DNA fragments is studied by a Hamiltonian model which treats the inter-strand and intra-strand forces at the level of the base pair. The elastic response of a set of homogeneous helices to externally applied forces…

Biomolecules · Quantitative Biology 2016-06-30 Marco Zoli

The cellular cytoplasm is organized into compartments. Phase separation is a simple manner to create membrane-less compartments in order to confine and localize particles like proteins. In many cases these particles are bound to fluctuating…

Statistical Mechanics · Physics 2020-09-16 G. David , J. -C. Walter , C. P. Broedersz , J. Dorignac , F. Geniet , A. Parmeggiani , N. -O. Walliser , J. Palmeri

Dynamics of nucleosomes, the building blocks of the chromatin, has crucial effects on expression, replication and repair of genomes in eukaryotes. Beside constant movements of nucleosomes by thermal fluctuations, ATP-dependent chromatin…

Biomolecules · Quantitative Biology 2020-01-28 Fatemeh Khodabandeh , Hashem Fatemi , Farshid Mohammad-Rafiee1

The in vivo free energy of looping double-stranded DNA by the lac repressor has a remarkable behavior whose origins are not fully understood. In addition to the intrinsic periodicity of the DNA double helix, the in vivo free energy has an…

Biomolecules · Quantitative Biology 2007-05-23 Leonor Saiz , Jose M. G. Vilar

Molecular motor proteins form the basis of cellular dynamics. Recently, notable efforts have led to the creation of their DNA-based mimics, which can carry out complex nanoscale motion. However, such functional analogues have not yet been…

Biological Physics · Physics 2021-05-14 Kerstin Goepfrich , Maximilian J. Urban , Christoph Frey , Ilia Platzman , Joachim P. Spatz , Na Liu

Although mechanical properties of DNA are well characterized at the kilo base-pair range, a number of recent experiments have suggested that DNA is more flexible at shorter length scales, which correspond to the regime that is crucial for…

Biomolecules · Quantitative Biology 2015-06-11 Agnes Noy , Ramin Golestanian

The unconstrained genomic DNA of bacteria forms a coil, which volume exceeds 1000 times the volume of the cell. Since prokaryotes lack a membrane-bound nucleus, in sharp contrast with eukaryotes, the DNA may consequently be expected to…

Biomolecules · Quantitative Biology 2015-09-09 Marc Joyeux