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We show how single-molecule unzipping experiments can provide strong evidence that the zero-force melting transition of long molecules of natural dsDNA should be classified as a phase transition of the higher-order type (continuous). We…

Soft Condensed Matter · Physics 2007-05-23 C. Brian Roland , Kristi Adamson Hatch , Mara Prentiss , Eugene I. Shakhnovich

Analysis of single-molecule micromanipulation experiments of DNA unzipping process shows some features of the force-distance curve, namely two consequent plateaus in the area of ${\sim}12-14pN$ dependent on nucleotide sequence structure, as…

Biological Physics · Physics 2017-02-14 O. O. Zdorevskyi , S. N. Volkov

A theory of the unzipping of double-stranded (ds) DNA is presented, and is compared to recent micromanipulation experiments. It is shown that the interactions which stabilize the double helix and the elastic rigidity of single strands (ss)…

Statistical Mechanics · Physics 2009-11-07 S. Cocco , R. Monasson , J. Marko

We study theoretically the unzipping of a double stranded DNA from a condensed globule state by an external force. At constant force, we find that the double stranded DNA unzips an at critical force Fc and the number of unzipped monomers M…

Soft Condensed Matter · Physics 2007-05-23 Pui-Man Lam , J. C. Levy

We present a critique and extension of the mean-field approach to the mechanical pulling transition in bound polymer systems. Our model is motivated by the theoretically and experimentally important examples of adsorbed polymers and…

Soft Condensed Matter · Physics 2009-11-07 Eran A. Mukamel , Eugene I. Shakhnovich

The force induced unzipping transition of a double stranded DNA is considered from a purely thermodynamic point of view. This analysis provides us with a set of relations that can be used to test microscopic theories and experiments. The…

Soft Condensed Matter · Physics 2015-06-18 Poulomi Sadhukhan , Somendra M. Bhattacharjee

A double stranded DNA molecule when pulled with a force acting on one end of the molecule can become either partially or completely unzipped depending on the magnitude of the force F. For a random DNA sequence, the number M of unzipped base…

Soft Condensed Matter · Physics 2007-05-23 Pui-Man Lam , J. C. S. Levy , Hanchen Huang

A study of the micromechanical unzipping of DNA in the framework of the Peyrard-Bishop-Dauxois model is presented. We introduce a Monte Carlo technique that allows accurate determination of the dependence of the unzipping forces on…

Soft Condensed Matter · Physics 2007-05-23 N. K. Voulgarakis , A. Redondo , A. R. Bishop , K. O. Rasmussen

The experimental measurement of correlation functions and critical exponents in disordered systems is key to testing renormalization group (RG) predictions. We mechanically unzip single DNA hairpins with optical tweezers, an experimental…

Disordered Systems and Neural Networks · Physics 2023-05-31 Cathelijne ter Burg , Paolo Rissone , Marc Rico-Pasto , Felix Ritort , Kay Joerg Wiese

We study by using Monte Carlo simulations the hysteresis in unzipping and rezipping of a double stranded DNA (dsDNA) by pulling its strands in opposite directions in the fixed force ensemble. The force is increased, at a constant rate from…

Soft Condensed Matter · Physics 2012-10-11 Rajeev Kapri

DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and…

We present an extensive theoretical investigation of the mechanical unzipping of double-stranded DNA under the influence of an applied force. In the limit of long polymers, there is a thermodynamic unzipping transition at a critical force…

Soft Condensed Matter · Physics 2009-11-07 David K. Lubensky , David R. Nelson

A DNA-protein complex modelled by a semiflexible chain and an attractive spherical core is studied in the situation when an external stretching force is acting on one end monomer of the chain while the other end monomer is kept fixed in…

Soft Condensed Matter · Physics 2009-11-10 Takahiro Sakaue , Hartmut Löwen

Dynamics of nucleosomes, the building blocks of the chromatin, has crucial effects on expression, replication and repair of genomes in eukaryotes. Beside constant movements of nucleosomes by thermal fluctuations, ATP-dependent chromatin…

Biomolecules · Quantitative Biology 2020-01-28 Fatemeh Khodabandeh , Hashem Fatemi , Farshid Mohammad-Rafiee1

We study the effect of random forces on a double stranded DNA in unzipping the two strands, analogous to the problem of an adsorbed polymer under a random force. The ground state develops bubbles of various lengths as the random force…

Soft Condensed Matter · Physics 2007-05-23 Rajeev Kapri , Somendra M. Bhattacharjee

Eukaryotic cells are often exposed to fluctuations in growth conditions as well as endogenous and exogenous stress-related agents. In addition, during development global patterns of gene transcription change dramatically, and these changes…

Genomics · Quantitative Biology 2008-12-23 John Herrick

DNA polymerases are an essential class of enzymes or molecular motors that catalyze processive DNA syntheses during DNA replications. A critical issue for DNA polymerases is their molecular mechanism of processive DNA replication. We have…

Soft Condensed Matter · Physics 2021-11-01 Yu-Ru Liu , Peng-Ye Wang , Wei Li , Ping Xie

We study the unzipping of double stranded DNA (dsDNA) by applying a pulling force at a fraction $s$ $(0 \le s \le 1)$ from the anchored end. From exact analytical and numerical results, the complete phase diagram is presented. The phase…

Soft Condensed Matter · Physics 2009-11-10 Rajeev Kapri , Somendra M. Bhattacharjee , Flavio Seno

The possibility of detecting mutations in a DNA from force measurements (as a first step towards sequence analysis) is discussed theoretically based on exact calculations. The force signal is associated with the domain wall separating the…

Statistical Mechanics · Physics 2009-11-07 Somendra M. Bhattacharjee , D. Marenduzzo

The unzipping of a double stranded DNA whose ends are subjected to a time dependent periodic force with frequency $\omega$ and amplitude $G$ is studied using Monte Carlo simulations. We obtain the dynamical order parameter, $Q$, defined as…

Soft Condensed Matter · Physics 2019-06-17 M. Suman Kalyan , Rajeev Kapri