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Bacterial heterogeneity is pivotal for adaptation to diverse environments, posing significant challenges in microbial diagnostics and therapeutic interventions. Recent advancements in high-resolution optical microscopy have revolutionized…
The Depth from Defocus (DFD) imaging technique for measuring the size and number concentration of particles in a dispersed two-phase flow has up to now been restricted to relatively sparse particle densities and to identifying only…
Optical imaging below the limit of light diffraction offers an unprecedented opportunity to study outlook, organization, interactions or in-situ functioning of sub-micrometer, highly transparent objects such as subcellular structures in…
We report both sub-diffraction-limited quantum metrology and quantum enhanced spatial resolution for the first time in a biological context. Nanoparticles are tracked with quantum correlated light as they diffuse through an extended region…
Metasurfaces offer unconventional control of light to shape optical wavefronts within two-dimensional nanoscale structures. A nanostructured metallic thin film can establish an exclusive relationship between its surface structure and…
Given the hyper-spectral imaging unique potentials in grasping the polymer characteristics of different materials, it is commonly used in sorting procedures. In a practical plastic sorting scenario, multiple plastic flakes may overlap which…
In digital holography, the coherent scattered light fields can be reconstructed volumetrically. By refocusing the fields to the sample planes, absorption and phase-shift profiles of sparsely distributed samples can be simultaneously…
The ability to spatially and temporally map nanoscale environments in situ over extended timescales would be transformative for biology, biomedicine, and bioengineering. All nanometer objects, from nanoparticles down to single proteins,…
Proteins are arguably the most important class of biomarkers for health diagnostic purposes. Label-free solid-state nanopore sensing is a versatile technique for sensing and analysing biomolecules such as proteins at single-molecule level.…
In-line holographic microscopy provides an unparalleled wealth of information about the properties of colloidal dispersions. Analyzing one colloidal particle's hologram with the Lorenz-Mie theory of light scattering yields the particle's…
Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…
We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample…
For several centuries, far-field optical microscopy has remained a key instrument in many scientific disciplines including physical, chemical and biomedical research. Nonetheless, far-field imaging has many limitations: the spatial…
Small object detection is a broadly investigated research task and is commonly conceptualized as a "pipeline-style" engineering process. In the upstream, images serve as raw materials for processing in the detection pipeline, where…
We introduce a new, highly sensitive, and simple heterodyne optical method for imaging individual non-fluorescent nano-objects. A two orders of magnitude improvement of the signal is achieved compared to previous methods. This allows for…
Atomically sharp 2D in-plane heterostructures with nanoscale interfaces provide a powerful platform for tailoring optical and electrical properties at the nanoscale, enabling novel device engineering and the exploration of new physical…
Identifying, visualising and ultimately tracking dynamically moving non-fluorescent nanoparticles in the presence of non-specific scattering is a long-standing challenge across the nano- and life-sciences. In this work we demonstrate that…
We demonstrate the measurement of nanoparticle shape by angularly resolved Rayleigh scattering of single optical levitated particles that are oriented in space via the trapping light in vacuum. This technique is applied to a range of…
Our visual perception of our surroundings is ultimately limited by the diffraction limit, which stipulates that optical information smaller than roughly half the illumination wavelength is not retrievable. Over the past decades, many…
This work presents Depth Anything V2. Without pursuing fancy techniques, we aim to reveal crucial findings to pave the way towards building a powerful monocular depth estimation model. Notably, compared with V1, this version produces much…