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Detection and characterization of individual nano-scale particles, virions, and pathogens are of paramount importance to human health, homeland security, diagnostic and environmental monitoring[1]. There is a strong demand for…

Optics · Physics 2011-07-06 Lina He , Sahin Kaya Ozdemir , Jiangang Zhu , Woosung Kim , Lan Yang

There is a growing need for biolabels that can be used in both optical and electron microscopies, are non-cytotoxic, and do not photobleach. Such biolabels could enable targeted nanoscale imaging of sub-cellular structures, and help to…

Biological Physics · Physics 2015-06-23 Mark A. Zurbuchen , Michael P. Lake , Sirus A. Kohan , Belinda Leung , Louis-S. Bouchard

Label-free detection techniques for single particles and molecules play an important role in basic science, disease diagnostics, and nanomaterial investigations. While traditional fluorescence-based methods offer powerful tools for single…

Optics · Physics 2024-08-20 Shuang Hao , Sartanee Suebka , Judith Su

Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural…

We demonstrate high-resolution non-iterative holographic coherent diffraction imaging with hard X-rays using a novel phase-shifting reference, fabricated by atomic layer deposition to produce nanosharp 3D structure. The method surpasses the…

Mesoscale and Nanoscale Physics · Physics 2016-10-17 Mirna Saliba , Jeroen Bosgra , Christoph Rau , Christian David , Aaron D. Parsons , Ulrich H. Wagner , Pierre Thibault

Absolute quantification of biological samples entails determining expression levels in precise numerical copies, offering enhanced accuracy and superior performance for rare templates. However, existing methodologies suffer from significant…

Quantitative Methods · Quantitative Biology 2023-09-06 Yuanyuan Wei , Sai Mu Dalike Abaxi , Nawaz Mehmood , Luoquan Li , Fuyang Qu , Guangyao Cheng , Dehua Hu , Yi-Ping Ho , Scott Wu Yuan , Ho-Pui Ho

Droplet microfluidics has emerged as a powerful platform allowing a large number of individual reactions to be carried out in spatially distinct microcompartments. Due to their small size, however, the spectroscopic characterisation of…

We present a simplified yet sophisticated variation to localised surface plasmon resonance spectroscopy, which makes use of naked or non-functionalised, nanoparticle templates. These nanoparticle templates, produced with a rapid and…

Instrumentation and Detectors · Physics 2017-02-02 J. L. Spear , N. Pliatsikas , N. Kalfagiannis , P. Patsalas , D. C. Koutsogeorgis

Particle size measurement is crucial in various applications, be it sizing droplets in inkjet printing or respiratory events, tracking particulate ejection in hypersonic impacts, or detecting floating target markers in free surface flows.…

Fluid Dynamics · Physics 2023-10-04 Saini Jatin Rao , Shubham Sharma , Saptarshi Basu , Cameron Tropea

Estimating depth from images nowadays yields outstanding results, both in terms of in-domain accuracy and generalization. However, we identify two main challenges that remain open in this field: dealing with non-Lambertian materials and…

Computer Vision and Pattern Recognition · Computer Science 2024-01-31 Pierluigi Zama Ramirez , Alex Costanzino , Fabio Tosi , Matteo Poggi , Samuele Salti , Stefano Mattoccia , Luigi Di Stefano

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint…

DNA labeling is a powerful tool in molecular biology and biotechnology that allows for the visualization, detection, and study of DNA at the molecular level. Under this paradigm, a DNA molecule is being labeled by specific k patterns and is…

Information Theory · Computer Science 2024-01-24 Dganit Hanania , Daniella Bar-Lev , Yevgeni Nogin , Yoav Shechtman , Eitan Yaakobi

Atomic resolution imaging in transmission electron microscopy (TEM) and scanning TEM (STEM) of light elements in electron-transparent materials has long been a challenge. Biomolecular materials, for example, are rapidly altered when…

Instrumentation and Detectors · Physics 2018-12-05 Fehmi S. Yasin , Tyler R. Harvey , Jordan J. Chess , Jordan S. Pierce , Colin Ophus , Peter Ercius , Benjamin J. McMorran

Particle tracking is a fundamental task in digital microscopy. Recently, machine-learning approaches have made great strides in overcoming the limitations of more classical approaches. The training of state-of-the-art machine-learning…

Deep learning has achieved spectacular performance in image and speech recognition and synthesis. It outperforms other machine learning algorithms in problems where large amounts of data are available. In the area of measurement technology,…

Quantitative Methods · Quantitative Biology 2019-08-20 Yueqin Li , Ata Mahjoubfar , Claire Lifan Chen , Kayvan Reza Niazi , Li Pei , Bahram Jalali

Sensors that are able to detect and track single unlabelled biomolecules are an important tool both to understand biomolecular dynamics and interactions at nanoscale, and for medical diagnostics operating at their ultimate detection limits.…

Optics · Physics 2016-11-15 N. P. Mauranyapin , L. S. Madsen , M. A. Taylor , M. Waleed , W. P. Bowen

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

Deep subspace clustering (DSC) algorithms face several challenges that hinder their widespread adoption across variois application domains. First, clustering quality is typically assessed using only the encoder's output layer, disregarding…

Computer Vision and Pattern Recognition · Computer Science 2025-04-28 Lovro Sindicic , Ivica Kopriva

Accurately estimating depth in 360-degree imagery is crucial for virtual reality, autonomous navigation, and immersive media applications. Existing depth estimation methods designed for perspective-view imagery fail when applied to…

Computer Vision and Pattern Recognition · Computer Science 2024-10-31 Ning-Hsu Wang , Yu-Lun Liu

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

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