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Diffusion-based super-resolution (SR) models have recently garnered significant attention due to their potent restoration capabilities. But conventional diffusion models perform noise sampling from a single distribution, constraining their…

Computer Vision and Pattern Recognition · Computer Science 2025-02-13 Chengcheng Wang , Zhiwei Hao , Yehui Tang , Jianyuan Guo , Yujie Yang , Kai Han , Yunhe Wang

Following recent advancements in multimode fiber (MMF), miniaturization of imaging endoscopes has proven crucial for minimally invasive surgery in vivo. Recent progress enabled by super-resolution imaging methods with a data-driven deep…

Optics · Physics 2023-07-12 Chuncheng Zhang , Tingting Liu , Zhihua Xie , Yu Wang , Tong Liu , Qian Chen , Xiubao Sui

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

Super-resolution fluorescence microscopy is of great interest in life science studies for visualizing subcellular structures at the nanometer scale. Among various kinds of super-resolution approaches, image scanning microscopy (ISM) offers…

Scanning electron microscopy (SEM) is indispensable in diverse applications ranging from microelectronics to food processing because it provides large depth-of-field images with a resolution beyond the optical diffraction limit. However,…

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…

Fluorescent molecules are versatile nanoscale emitters that enable detailed observations of biophysical processes with nanoscale resolution. Because they are well-approximated as electric dipoles, imaging systems can be designed to…

Optics · Physics 2024-03-26 Tingting Wu , Matthew D. Lew

Single-molecule localization microscopy (SMLM) allows reconstructing biology-relevant structures beyond the diffraction limit by detecting and localizing individual fluorophores -- fluorescent molecules stained onto the observed specimen --…

Computer Vision and Pattern Recognition · Computer Science 2026-03-03 Romain Seailles , Jean-Baptiste Masson , Jean Ponce , Julien Mairal

Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…

Optics · Physics 2025-08-29 Lukas Scheiderer , Zach Marin , Jonas Ries

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

Deep neural networks have recently achieved significant advancements in remote sensing superresolu-tion (SR). However, most existing methods are limited to low magnification rates (e.g., 2 or 4) due to the escalating ill-posedness at higher…

Image and Video Processing · Electrical Eng. & Systems 2024-12-30 Yue Shi , Liangxiu Han , Darren Dancy , Lianghao Han

Super-resolution imaging with advanced optical systems has been revolutionizing technical analysis in various fields from biological to physical sciences. However, many objects are hidden by strongly scattering media such as rough wall…

Optics · Physics 2021-09-06 Dong Wang , Sujit Kumar Sahoo , Cuong Dang

Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution…

Recent score-based diffusion models (SBDMs) show promising results in unpaired image-to-image translation (I2I). However, existing methods, either energy-based or statistically-based, provide no explicit form of the interfered intermediate…

Computer Vision and Pattern Recognition · Computer Science 2023-08-07 Shikun Sun , Longhui Wei , Junliang Xing , Jia Jia , Qi Tian

While electron microscopy offers crucial atomic-resolution insights into structure-property relationships, radiation damage severely limits its use on beam-sensitive materials like proteins and 2D materials. To overcome this challenge, we…

Computer Vision and Pattern Recognition · Computer Science 2025-07-18 Zifei Wang , Zian Mao , Xiaoya He , Xi Huang , Haoran Zhang , Chun Cheng , Shufen Chu , Tingzheng Hou , Xiaoqin Zeng , Yujun Xie

Solving medical imaging data scarcity through semantic image generation has attracted growing attention in recent years. However, existing generative models mainly focus on synthesizing whole-organ or large-tissue structures, showing…

Image and Video Processing · Electrical Eng. & Systems 2025-12-19 Jiahao Xia , Yutao Hu , Yaolei Qi , Zhenliang Li , Wenqi Shao , Junjun He , Ying Fu , Longjiang Zhang , Guanyu Yang

Measuring subdiffraction separations between single fluorescent particles is important for biological, nano-, and medical-technology studies. Major challenges include (i) measuring changing molecular separations with high temporal…

Biological Physics · Physics 2015-05-18 Shawn H. DeCenzo , Michael C. DeSantis , Y. M. Wang

In Super-resolution, a varying-illumination image stack is required. This enriched the dataset typically necessitates precise mechanical control and micron scale optical alignment and repeatability. Here, we introduce a novel methodology…

Advances in microscopy imaging enable researchers to visualize structures at the nanoscale level thereby unraveling intricate details of biological organization. However, challenges such as image noise, photobleaching of fluorophores, and…

Image and Video Processing · Electrical Eng. & Systems 2024-09-19 Pamela Osuna-Vargas , Maren H. Wehrheim , Lucas Zinz , Johanna Rahm , Ashwin Balakrishnan , Alexandra Kaminer , Mike Heilemann , Matthias Kaschube

Despite super-resolution fluorescence blinking microscopes break the diffraction limit, the intense phototoxic illumination and long-term image sequences thus far still pose to major challenges in visualizing live-organisms. Here, we…