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We present a widefield fluorescence microscope that integrates an event-based image sensor (EBIS) with a CMOS image sensor (CIS) for ultra-fast microscopy with spectral distinction capabilities. The EBIS achieves temporal resolution of…

Optics · Physics 2024-10-22 Richard G. Baird , Apratim Majumder , Rajesh Menon

Fluorescence microscopy is widely employed for the analysis of living biological samples; however, the utility of the resulting recordings is frequently constrained by noise, temporal variability, and inconsistent visualisation of signals…

Computer Vision and Pattern Recognition · Computer Science 2026-01-16 Hassan Eshkiki , Sarah Costa , Mostafa Mohammadpour , Farinaz Tanhaei , Christopher H. George , Fabio Caraffini

Event cameras provide asynchronous, data-driven measurements of local temporal contrast over a large dynamic range with extremely high temporal resolution. Conventional cameras capture low-frequency reference intensity information. These…

Computer Vision and Pattern Recognition · Computer Science 2018-11-02 Cedric Scheerlinck , Nick Barnes , Robert Mahony

Imaging across both the full transverse spatial and temporal dimensions of a scene with high precision in all three coordinates is key to applications ranging from LIDAR to fluorescence lifetime imaging. However, compromises that sacrifice,…

Image and Video Processing · Electrical Eng. & Systems 2021-01-12 C. Callenberg , A. Lyons , D. den Brok , A. Fatima , A. Turpin , V. Zickus , L. Machesky , J. Whitelaw , D. Faccio , M. B. Hullin

Most existing RGB-based trackers target low frame rate benchmarks of around 30 frames per second. This setting restricts the tracker's functionality in the real world, especially for fast motion. Event-based cameras as bioinspired sensors…

Computer Vision and Pattern Recognition · Computer Science 2023-05-26 Jiqing Zhang , Yuanchen Wang , Wenxi Liu , Meng Li , Jinpeng Bai , Baocai Yin , Xin Yang

We report, for the first time, a multi-confocal Fluorescence Correlation Spectroscopy (mFCS) technique which allows parallel measurements at different locations, by combining a Spatial Light Modulator (SLM), with an Electron Multiplying-CCD…

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

Microscopes face a trade-off between spatial resolution, field-of-view, and frame rate -- improving one of these properties typically requires sacrificing the others, due to the limited spatiotemporal throughput of the sensor. To overcome…

Standard frame-based cameras that sample light intensity frames are heavily impacted by motion blur for high-speed motion and fail to perceive scene accurately when the dynamic range is high. Event-based cameras, on the other hand, overcome…

Computer Vision and Pattern Recognition · Computer Science 2021-03-22 Chankyu Lee , Adarsh Kumar Kosta , Kaushik Roy

Neuromorphic "event" cameras, designed to mimic the human vision system with asynchronous sensing, unlock a new realm of high-speed and high dynamic range applications. However, researchers often either revert to a framed representation of…

Computer Vision and Pattern Recognition · Computer Science 2023-12-14 Andrew C. Freeman , Montek Singh , Ketan Mayer-Patel

Event cameras or dynamic vision sensors (DVS) record asynchronous response to brightness changes instead of conventional intensity frames, and feature ultra-high sensitivity at low bandwidth. The new mechanism demonstrates great advantages…

Computer Vision and Pattern Recognition · Computer Science 2024-01-09 Bo Zhang , Yuqi Han , Jinli Suo , Qionghai Dai

Recently, video frame interpolation using a combination of frame- and event-based cameras has surpassed traditional image-based methods both in terms of performance and memory efficiency. However, current methods still suffer from (i)…

Computer Vision and Pattern Recognition · Computer Science 2022-04-26 Stepan Tulyakov , Alfredo Bochicchio , Daniel Gehrig , Stamatios Georgoulis , Yuanyou Li , Davide Scaramuzza

Time-resolved fluorescence imaging is a key tool in biomedical applications, as it allows to non-invasively obtain functional and structural information. However, the big amount of collected data introduces challenges in both acquisition…

The inherent non-linearity of intensity correlation functions can be used to spatially distinguish identical emitters beyond the diffraction limit, as achieved, for example, in Super-Resolution Optical Fluctuation Imaging (SOFI). Here, we…

Optics · Physics 2024-12-18 Yifan Chen , Chieh Tsao , Hendrik Utzat

Fluorescence imaging is the most widely used method for unveiling the molecular composition of biological specimens. However, the weak optical emission of fluorescent probes and the tradeoff between imaging speed and sensitivity is…

Current optical flow methods exploit the stable appearance of frame (or RGB) data to establish robust correspondences across time. Event cameras, on the other hand, provide high-temporal-resolution motion cues and excel in challenging…

Computer Vision and Pattern Recognition · Computer Science 2025-08-20 Qianang Zhou , Junhui Hou , Meiyi Yang , Yongjian Deng , Youfu Li , Junlin Xiong

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

We present a method that leverages the complementarity of event cameras and standard cameras to track visual features with low-latency. Event cameras are novel sensors that output pixel-level brightness changes, called "events". They offer…

Computer Vision and Pattern Recognition · Computer Science 2019-01-21 Daniel Gehrig , Henri Rebecq , Guillermo Gallego , Davide Scaramuzza

Scanning fluorescence correlation spectroscopy (SFCS) with a scan path perpendicular to the membrane plane was introduced to measure diffusion and interactions of fluorescent components in free standing biomembranes. Using a confocal laser…

Biomolecules · Quantitative Biology 2018-06-04 Paul Müller , Petra Schwille , Thomas Weidemann

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio
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