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Background: Spatial transcriptomics have emerged as a powerful tool in biomedical research because of its ability to capture both the spatial contexts and abundance of the complete RNA transcript profile in organs of interest. However,…

Genomics · Quantitative Biology 2025-04-18 Shuo Shuo Liu , Shikun Wang , Yuxuan Chen , Anil K. Rustgi , Ming Yuan , Jianhua Hu

Spatial transcriptomics (ST) is a groundbreaking genomic technology that enables spatial localization analysis of gene expression within tissue sections. However, it is significantly limited by high costs and sparse spatial resolution. An…

Image and Video Processing · Electrical Eng. & Systems 2024-07-31 Zhiceng Shi , Shuailin Xue , Fangfang Zhu , Wenwen Min

Spatial transcriptomics (ST) technologies can be used to align transcriptomes with histopathological morphology, presenting exciting new opportunities for biomolecular discovery. Using ST data, we construct a novel framework, GeneFlow, to…

Quantitative Methods · Quantitative Biology 2025-11-04 Mengbo Wang , Shourya Verma , Aditya Malusare , Luopin Wang , Yiyang Lu , Vaneet Aggarwal , Mario Sola , Ananth Grama , Nadia Atallah Lanman

Spatial Transcriptomics (ST) is a method that captures gene expression profiles aligned with spatial coordinates. The discrete spatial distribution and the super-high dimensional sequencing results make ST data challenging to be modeled…

Machine Learning · Computer Science 2025-05-08 Qingtian Zhu , Yumin Zheng , Yuling Sang , Yifan Zhan , Ziyan Zhu , Jun Ding , Yinqiang Zheng

Advances in spatially-resolved transcriptomics (SRT) technologies have propelled the development of new computational analysis methods to unlock biological insights. As the cost of generating these data decreases, these technologies provide…

Genomics · Quantitative Biology 2024-08-02 Boyi Guo , Wodan Ling , Sang Ho Kwon , Pratibha Panwar , Shila Ghazanfar , Keri Martinowich , Stephanie C. Hicks

Spatially resolved transcriptomics (ST) measures gene expression along with the spatial coordinates of the measurements. The analysis of ST data involves significant computation complexity. In this work, we propose gene expression…

Genomics · Quantitative Biology 2022-05-24 Zhuoyan Xu , Kris Sankaran

Spatial transcriptomics enables genome-wide expression analysis within native tissue context, yet identifying spatial domains remains challenging due to complex gene-spatial interactions. Existing methods typically process spatial and…

Machine Learning · Computer Science 2025-12-19 Jianping Mei , Siqi Ai , Ye Yuan

Spatial transcriptomics data analysis integrates cellular transcriptional activity with spatial coordinates to identify spatial domains, infer cell-type dynamics, and characterize gene expression patterns within tissues. Despite recent…

Quantitative Methods · Quantitative Biology 2026-03-25 Sean Cottrell , Guo-Wei Wei , Longxiu Huang

Advances in spatial transcriptomics (ST) technologies enable systematic molecular characterization of tumor microenvironment, tumor gradients and gene regulatory networks. Cancer progression is known to vary along pathological gradients,…

Cellular identity and function are linked to both their intrinsic genomic makeup and extrinsic spatial context within the tissue microenvironment. Spatial transcriptomics (ST) offers an unprecedented opportunity to study this, providing in…

Machine Learning · Computer Science 2026-02-16 Rui Yan , Xiaohan Xing , Xun Wang , Zixia Zhou , Md Tauhidul Islam , Lei Xing

With the rapid advancement of Spatial Resolved Transcriptomics (SRT) technology, it is now possible to comprehensively measure gene transcription while preserving the spatial context of tissues. Spatial domain identification and gene…

Genomics · Quantitative Biology 2024-08-14 Donghai Fang , Fangfang Zhu , Dongting Xie , Wenwen Min

Spatial transcriptomics (ST) is an emerging technology that enables researchers to investigate the molecular relationships underlying tissue morphology. However, acquiring ST data remains prohibitively expensive, and traditional fixed-grid…

Computer Vision and Pattern Recognition · Computer Science 2026-02-03 Junchao Zhu , Ruining Deng , Junlin Guo , Tianyuan Yao , Chongyu Qu , Juming Xiong , Siqi Lu , Zhengyi Lu , Yanfan Zhu , Marilyn Lionts , Yuechen Yang , Yalin Zheng , Yu Wang , Shilin Zhao , Haichun Yang , Yuankai Huo

Spatial Transcriptomics (ST) enables the measurement of gene expression while preserving spatial information, offering critical insights into tissue architecture and disease pathology. Recent developments have explored the use of…

Computer Vision and Pattern Recognition · Computer Science 2026-05-20 Hai Dang Nguyen , Nguyen Dang Huy Pham , The Minh Duc Nguyen , Dac Thai Nguyen , Hang Thi Nguyen , Duong M. Nguyen

Spatial transcriptomics is a modern sequencing technology that allows the measurement of the activity of thousands of genes in a tissue sample and map where the activity is occurring. This technology has enabled the study of the so-called…

Methodology · Statistics 2022-09-15 Andrea Sottosanti , Davide Risso

The spatial location of cells within tissues and organs is crucial for the manifestation of their specific functions.Spatial transcriptomics technology enables comprehensive measurement of the gene expression patterns in tissues while…

Quantitative Methods · Quantitative Biology 2024-07-12 Shuailin Xue , Fangfang Zhu , Changmiao Wang , Wenwen Min

This paper proposes a novel spatiotemporal (ST) fusion framework for satellite images, named Robust Optimization-based Spatiotemporal Fusion (ROSTF). ST fusion is a promising approach to resolve a trade-off between the temporal and spatial…

Image and Video Processing · Electrical Eng. & Systems 2024-07-01 Ryosuke Isono , Kazuki Naganuma , Shunsuke Ono

Spatial transcriptomic (ST) clustering employs spatial and transcription information to group spots spatially coherent and transcriptionally similar together into the same spatial domain. Graph convolution network (GCN) and graph attention…

Quantitative Methods · Quantitative Biology 2023-10-24 Chen Zhang , Junhui Gao , Lingxin Kong , Guangshuo cao , Xiangyu Guo , Wei Liu

Spatial transcriptomics (ST) provides crucial insights into tissue micro-environments, but is limited to its high cost and complexity. As an alternative, predicting gene expression from pathology whole slide images (WSI) is gaining…

Computer Vision and Pattern Recognition · Computer Science 2025-07-01 Mingcheng Qu , Yuncong Wu , Donglin Di , Yue Gao , Tonghua Su , Yang Song , Lei Fan

The rapid advancement of spatial transcriptomics (ST), i.e., spatial gene expressions, has made it possible to measure gene expression within original tissue, enabling us to discover molecular mechanisms. However, current ST platforms…

Computer Vision and Pattern Recognition · Computer Science 2025-11-11 Xiaofei Wang , Stephen Price , Chao Li

Accurate detection of cancer tissue regions (CTR) enables deeper analysis of the tumor microenvironment and offers crucial insights into treatment response. Traditional CTR detection methods, which typically rely on the rich cellular…

Computer Vision and Pattern Recognition · Computer Science 2026-03-09 Shuailin Xue , Jun Wan , Lihua Zhang , Wenwen Min