Related papers: Label-free quantitative imaging of two-dimensional…
Molecular dipoles near interfaces emit highly directional radiation due to near-field interactions, making surface-bound fluorophores sensitive probes of local physicochemical changes. We introduce smart coverslips, stably coated with…
Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…
In the paper, we explain an automated LabVIEW controlled setup that enables interferometric measurements of refractive indices in crystalline materials using a laser light source. The setup combines a mechanical system, a…
A novel method is proposed to measure the refractive indices (RIs) of the materials of different transparent solid state media. To exploit the advantage of non-contact measurement laser beam interferometry is used as an effective technique…
Diffractometric biosensing is a promising technology to overcome critical limitations of refractometric biosensors, the dominant class of label-free optical transducers. These limitations manifest themselves by higher noise and drifts due…
Within the last decade microscopic multiphase flows have gained broad interest. An exact understanding of the underlying hydrodynamic interrelations is the key for successful reactor layout and reaction control. To examine the local…
Non-invasive morphological assessment is the cornerstone of oocyte and embryo selection in assisted reproductive technology, yet clinical practice remains limited by two-dimensional, qualitative microscopy. While three-dimensional (3D)…
We have implemented a multi-functional optofluidic sensor that can monitor changes in the refractive index and pressure of biofluid simultaneously and can detect free-solution molecular interaction in-situ. In this paper, we demonstrate two…
Refractive index sensing is a highly sensitive and label-free detection method for molecular binding events. Commercial implementations of biosensing concepts based on plasmon resonances typically require significant external…
In this work, a refractive index (RI) sensor with an effective integration of colorimetric detection and optical sensing capabilities has been developed. Colorimetric detection relies on the sensitivity of the structural color of photonic…
Three-dimensional (3D) imaging of the subcellular organisation and morphology of cells and tissues is essential for understanding biological function. Although staining is the most widely used approach for visualising biological samples…
Optical microscopy has been employed to derive salient characteristics of an object in various fields, including cell biology, flow cytometry, biopsy, and neuroscience. In particular, measuring the phase of light scattered from an object…
Optical fibre-based sensors measuring refractive index shift in bodily fluids and tissues are versatile and accurate probes of physiological processes. Here, we suggest a refractive index sensor based on a microstructured exposed-core fibre…
One way of optically monitoring molecule concentrations is to utilise the high sensitivity of the transmission and reflection rates of Fabry-Perot cavities to changes of their optical properties. Up to now, intrinsic and extrinsic…
Total internal reflection fluorescence (TIRF) microscopy and its variants are key technologies for visualizing the dynamics of single molecules or organelles in live cells. Yet, truely quantitative TIRF remains problematic. One unknown…
This research focuses on a coherently driven four-level atomic medium with the aim of inducing a negative index of refraction while taking into consideration local field corrections as well as magnetoelectric cross coupling, i.e. chirality,…
Optical diffraction tomography (ODT) has emerged as a powerful label-free three-dimensional (3D) bioimaging techniques for observing living cells and thin tissue layers. We report a new reflection-mode ODT (rODT) method for imaging thick…
Mid-infrared spectroscopy is essential for chemical identification and compositional analysis, due to the existence of characteristic molecular absorption fingerprints. However, it is very challenging to determine the refractive index of an…
Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…
We developed a microscopy technique that can measure the local refractive index without sampling the optical phase delay of the electromagnetic radiation. To do this, we designed and experimentally demonstrated a setup with two co-localized…