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Refractive index matching (RIM) is a powerful tool for multiphase flow studies as it eliminates optical distortions and enables high-fidelity tomographic measurements near solid-fluid interfaces of freely moving solids in the flow. However,…

Fluid Dynamics · Physics 2026-02-19 Jibu Tom Jose , Aviel Ben-Harosh , Omri Ram

Label-free biosensors enable the monitoring of biomolecular interactions in real-time, which is key to the analysis of the binding characteristics of biomolecules. While refractometric optical biosensors are sensitive and well-established,…

High-throughput label-free measurements of the optical and mechanical properties of single microparticles play an important role in biological research, drug development, and related large population assays. Mechanical detection techniques…

Instrumentation and Detectors · Physics 2018-12-05 Kewen Han , Jeewon Suh , Gaurav Bahl

We proposed a refractive index (RI) sensing method with temperature compensation by using an optical frequency comb (OFC) sensing cavity employing a multimode-interference (MMI) fiber, namely, the MMI-OFC sensing cavity. The MMI-OFC sensing…

Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous…

Biological Physics · Physics 2019-04-10 Neelam Hari , Priyanka Patel , Jacqueline Ross , Kevin Hicks , Frédérique Vanholsbeeck

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

In the present work a new model of a refractive index (RI) sensor is exhibited. This is based on a dual core micro-structured optical fiber (MOF), where two holes are introduced at the core centers. In this way, the model enhances the…

Optics · Physics 2018-03-16 G. Tsigaridas , V. Karvouniaris , G. Chalkiadakis , P. Persephonis

In this paper, we report a capillary-based M-Z interferometer that could be used for precise detection of variations in refractive indices of gaseous samples. This sensing mechanism is quite straightforward. Cladding and core modes of a…

Applied Physics · Physics 2020-03-10 Haijin Chen , Xuehao Hu , Meifan He , Pengfei Ren , Chao Zhang , Hang Qu

Optical detection of individual nanometer-sized analytes, virus particles, and protein molecules holds great promise for understanding and control of biological samples and healthcare applications. As fluorescent labels impose restrictions…

Optics · Physics 2020-10-19 Martin Dieter Baaske , Peter Sebastian Neu , Michel Orrit

Interferometric scattering (iSCAT) microscopy is a powerful tool for label-free sensitive detection and imaging of nanoparticles to high spatio-temporal resolution. As it was born out of detection principles central to conventional…

Optics · Physics 2018-12-31 Richard W. Taylor , Vahid Sandoghdar

The characterization of white blood cells (WBCs) is crucial for blood analyses and disease diagnoses. However, current standard techniques rely on cell labeling, a process which imposes significant limitations. Here we present…

Biological Physics · Physics 2015-05-12 Jonghee Yoon , Kyoohyun Kim , HyunJoo Park , Chulhee Choi , Seongsoo Jang , YongKeun Park

White light spectral interferometry is applied to measure the refractive index in absorbing liquids in the spectral range from 400 to 1000 nm. We analyze the influence of absorption on the visibility of interferometric fringes and,…

Reflection phase imaging provides label-free, high-resolution characterization of biological samples, typically using interferometric-based techniques. Here, we investigate reflection phase microscopy from intensity-only measurements under…

Quantitative Methods · Quantitative Biology 2019-12-18 Alex Matlock , Anne Sentenac , Patrick C. Chaumet , Ji Yi , Lei Tian

Digital holographic microscopy is a thriving imaging modality that attracted considerable research interest in quantitative biological cell imaging due to its ability to not only create excellent label-free contrast, but also supply…

Optics · Physics 2019-04-03 Gili Dardikman , Natan T. Shaked

Refractive index is a fundamental optical property of powder and a key input to the measurement of the size distribution using light scattering and the measurement of the absorption and scattering coefficients using diffuse reflectance…

Optics · Physics 2024-03-05 Cory Juntunen , Adam J. Rish , Carl Anderson , Yongjin Sung

Intensity diffraction tomography (IDT) refers to a class of optical microscopy techniques for imaging the 3D refractive index (RI) distribution of a sample from a set of 2D intensity-only measurements. The reconstruction of artifact-free RI…

Image and Video Processing · Electrical Eng. & Systems 2022-08-16 Renhao Liu , Yu Sun , Jiabei Zhu , Lei Tian , Ulugbek Kamilov

Simultaneous imaging of fluorescence-labeled and label-free phase objects in the same sample provides distinct and complementary information. Most multimodal fluorescence-phase imaging operates in transmission mode, capturing fluorescence…

Optics · Physics 2024-08-20 Renzhi He , Yucheng Li , Junjie Chen , Yi Xue

Accessing the low-energy non-equilibrium dynamics of materials and their polaritons with simultaneous high spatial and temporal resolution has been a bold frontier of electron microscopy in recent years. One of the main challenges lies in…

We demonstrate novel instrumentation for spontaneous Raman spectroscopy in biofluids, enabling development of a portable, automated, reliable diagnostics technique requiring minimal operator expertise to quantify disease markers. Label-free…

Biological Physics · Physics 2021-05-27 Emily E. Storey , Duxuan Wu , Amr S. Helmy

Portable and cost-effective gas sensors are gaining demand for a number of environmental, biomedical and industrial applications, yet current devices are confined into specialized labs and cannot be extended to general use. Here, we…

Instrumentation and Detectors · Physics 2020-04-10 Giuseppe Antonacci , Jeroen Goyvaerts , Haolan Zhao , Bettina Baumgartner , Bernhard Lendl , Roel Baets