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Optical diffraction tomography (ODT) enables non-invasive information-rich 3D refractive index (RI) reconstruction of unimpaired transparent biological and technical samples, crucial in biomedical research, optical metrology, materials…

Electron cryo-tomography (cryo-ET) enables 3D imaging of complex, radiation-sensitive structures with molecular detail. However, image contrast from the interference of scattered electrons is nonlinear with atomic density and multiple…

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful…

We introduce Appearance-MAT (AMAT), a generalization of the medial axis transform for natural images, that is framed as a weighted geometric set cover problem. We make the following contributions: i) we extend previous medial point…

Computer Vision and Pattern Recognition · Computer Science 2017-08-04 Stavros Tsogkas , Sven Dickinson

Attosecond science offers unprecedented precision in probing the initial moments of chemical reactions, revealing the dynamics of molecular electrons that shape reaction pathways. A fundamental question emerges: what role, if any, do…

Chemical Physics · Physics 2024-07-24 Varun Makhija , Rishabh Gupta , Simon Neville , Micheal Schuurman , Joseph Francisco , Sabre Kais

Quantitative phase imaging (QPI) is a label-free computational imaging technique used in various fields, including biology and medical research. Modern QPI systems typically rely on digital processing using iterative algorithms for phase…

Optics · Physics 2023-06-28 Yuhang Li , Yi Luo , Deniz Mengu , Bijie Bai , Aydogan Ozcan

Quantum entanglement and squeezing have significantly improved phase estimation and imaging in interferometric settings beyond the classical limits. However, for a wide class of non-interferometric phase imaging/retrieval methods vastly…

Miniaturized photodetectors are becoming increasingly sought-after components for a range of next generation technologies, such as autonomous vehicles, integrated wearable devices, or gadgets embedded in the Internet of Things. A major…

Histological staining of tissue biopsies, especially hematoxylin and eosin (H&E) staining, serves as the benchmark for disease diagnosis and comprehensive clinical assessment of tissue. However, the process is laborious and time-consuming,…

Quantitative phase microscopy (QPM), a technique combining phase imaging and microscopy, enables visualization of the 3D topography in reflective samples, as well as the inner structure or refractive index distribution of transparent and…

Optics · Physics 2025-01-17 Vicente Mico , Juanjuan Zheng , Javier Garcia , Zeev Zalevsky , Peng Gao

Localization-based imaging has revolutionized fluorescence optical microscopy and has also enabled unprecedented ultrasound images of microvascular structures in deep tissues. Herein, we introduce a new concept of localization optoacoustic…

Optics · Physics 2017-07-10 Xose Luis Dean-Ben , Daniel Razansky

Quantum lithography (QL) has been suggested as a means of achieving enhanced spatial resolution for optical imaging, but its realization has been held back by the low multi-photon detection rates of recording materials. Recently, an optical…

Quantum Physics · Physics 2015-05-28 Heedeuk Shin , Kam Wai Clifford Chan , Hye Jeong Chang , Robert W. Boyd

We propose a technique to obtain sub-wavelength resolution in quantum imaging with potentially 100% contrast using incoherent light. Our method requires neither path-entangled number states nor multi-photon absorption. The scheme makes use…

Quantum Physics · Physics 2009-11-13 C. Thiel , T. Bastin , J. Martin , E. Solano , J. von Zanthier , G. S. Agarwal

We demonstrate a three-dimensional (3D) optical diffraction tomographic technique with optimal frequency combination (OFC-ODT) for the 3D quantitative phase imaging of unlabeled specimens. Three sets of through-focus intensity images are…

Optics · Physics 2018-03-06 Jiaji Li , Qian Chen Jiasong Sun , Jialin Zhang , Junyi Ding , Chao Zuo

Absorption imaging is a commonly adopted method to acquire, with high temporal resolution, spatial information on a partially transparent object. It relies on the interference between a probe beam and the coherent response of the object. In…

Optical coherence tomography (OCT) is capable of non-destructively obtaining cross-sectional information of samples with micrometer spatial resolution, which plays an important role in ophthalmology and endovascular medicine. Measuring OCT…

Medical Physics · Physics 2023-11-30 Yang Jianlong , Zhang Haoran , Liu Chang , Gu Chengfu

The principles of quantum optics have yielded a plethora of ideas to surpass the classical limitations of sensitivity and resolution in optical microscopy. While some ideas have been applied in proof-of-principle experiments, imaging a…

Optical coherence tomography (OCT) is a widely used imaging technique in the micrometer regime, which gained accelerating interest in medical imaging in the last twenty years. In up-to-date OCT literature [5,6] certain simplifying…

Medical Physics · Physics 2024-02-23 Leopold Veselka , Lisa Krainz , Leonidas Mindrinos , Wolfgang Drexler , Peter Elbau

We present a novel approach for benchmarking and validating quantitative phase tomography (QPT) systems using three-dimensional microphantoms. These microphantoms, crafted from biological and imaging data, replicate the optical and…

Quantitative phase imaging (QPI) recovers the exact wavefront of light from the intensity measured by a camera. Topographical maps of translucent microscopic bodies can be extracted from these quantified phase shifts. We demonstrate…