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Imaging in thick biological tissues is often degraded by sample-induced aberrations, which reduce image quality and resolution, particularly in super-resolution techniques. While hardware-based adaptive optics, which correct aberrations…

Fluorescence polarization microscopy images both the intensity and orientation of fluorescent dipoles, which plays a vital role in studying the molecular structure and dynamics of bio-complex. However, it is difficult to resolve the dipole…

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

Diffusion magnetic resonance imaging (dMRI) often suffers from low spatial and angular resolution due to inherent limitations in imaging hardware and system noise, adversely affecting the accurate estimation of microstructural parameters…

Image and Video Processing · Electrical Eng. & Systems 2025-01-28 Ruoyou Wu , Jian Cheng , Cheng Li , Juan Zou , Wenxin Fan , Hua Guo , Yong Liang , Shanshan Wang

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

Based on image encoding in a serial-temporal format, optical time-stretch imaging entails a stringent requirement of state-of-the- art fast data acquisition unit in order to preserve high image resolution at an ultrahigh frame rate ---…

Instrumentation and Detectors · Physics 2017-03-29 Antony C. S. Chan , Ho-Cheung Ng , Sharat C. V. Bogaraju , Hayden K. H. So , Edmund Y. Lam , Kevin K. Tsia

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

Light field microscopy (LFM) has been widely utilized in various fields for its capability to efficiently capture high-resolution 3D scenes. Despite the rapid advancements in neural representations, there are few methods specifically…

Image and Video Processing · Electrical Eng. & Systems 2024-09-30 Jiayin Zhao , Zhifeng Zhao , Jiamin Wu , Tao Yu , Hui Qiao

To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve…

We propose FlowReg, a deep learning-based framework for unsupervised image registration for neuroimaging applications. The system is composed of two architectures that are trained sequentially: FlowReg-A which affinely corrects for gross…

Computer Vision and Pattern Recognition · Computer Science 2021-09-02 Sergiu Mocanu , Alan R. Moody , April Khademi

Single-beam scanning microscopy (SBSM) is one of the most robust strategies for commercial optical systems. Although structured illumination combined with Fourier-domain spatial spectrum fusion can enhance SBSM resolution beyond the…

Optics · Physics 2025-07-15 Yongtao Liu , Tianxiao Wu , Xiao Zhou , Fan Wang

Fourier Ptychographic Microscopy (FPM) is a computational imaging method that is able to super-resolve features beyond the diffraction-limit set by the objective lens of a traditional microscope. This is accomplished by using synthetic…

Signal Processing · Electrical Eng. & Systems 2019-04-09 Michael Kellman , Emrah Bostan , Michael Chen , Laura Waller

Structured Illumination Microscopy (SIM) allows access to spatial information beyond the diffraction limit by folding high frequency components into the optical system's base-band. Using various algorithmic techniques, an image containing…

Optics · Physics 2024-10-16 Doron Shterman , Guy Bartal

Despite their widespread use in cell biology, fluorescence lifetime imaging microscopy (FLIM) data-sets are challenging to analyse, because each spatial position can contain a superposition of multiple fluorescent components. Here, we…

Biological Physics · Physics 2022-08-29 Francesco Masia , Walter Dewitte , Paola Borri , Wolfgang Langbein

Acoustic-Resolution Photoacoustic Microscopy (AR-PAM) is promising for subcutaneous vascular imaging, but its spatial resolution is constrained by the Point Spread Function (PSF). Traditional deconvolution methods like Richardson-Lucy and…

Computer Vision and Pattern Recognition · Computer Science 2024-10-29 Youshen Xiao , Sheng Liao , Xuanyang Tian , Fan Zhang , Xinlong Dong , Yunhui Jiang , Xiyu Chen , Ruixi Sun , Yuyao Zhang , Fei Gao

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

Structured illumination microscopy (SIM) is an optical super-resolution technique that enables live-cell imaging beyond the diffraction limit. Reconstruction of SIM data is prone to artefacts, which becomes problematic when imaging highly…

Image and Video Processing · Electrical Eng. & Systems 2022-03-02 Charles N. Christensen , Meng Lu , Edward N. Ward , Pietro Lio , Clemens F. Kaminski

Developing reliable and generalizable deep learning systems for medical imaging faces significant obstacles due to spurious correlations, data imbalances, and limited text annotations in datasets. Addressing these challenges requires…

Computer Vision and Pattern Recognition · Computer Science 2025-07-23 Amar Kumar , Anita Kriz , Mohammad Havaei , Tal Arbel

Single-pixel imaging has emerged as a key technique in fluorescence microscopy, where fast acquisition and reconstruction are crucial. In this context, images are reconstructed from linearly compressed measurements. In practice, total…

Image and Video Processing · Electrical Eng. & Systems 2025-07-28 Serban C. Tudosie , Valerio Gandolfi , Shivaprasad Varakkoth , Andrea Farina , Cosimo D'Andrea , Simon Arridge

Semantic segmentation is a pixel-level prediction task to classify each pixel of the input image. Deep learning models, such as convolutional neural networks (CNNs), have been extremely successful in achieving excellent performances in this…

Computer Vision and Pattern Recognition · Computer Science 2023-02-24 Nadeem Atif , Saquib Mazhar , Debajit Sarma , M. K. Bhuyan , Shaik Rafi Ahamed