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Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution is limited by the fluorescence lifetime to nanoseconds at most. At the ensemble level, two-dimensional spectroscopy…

Optics · Physics 2024-10-11 Sanchayeeta Jana , Simon Durst , Markus Lippitz

We propose a compact snapshot monocular depth estimation technique that relies on an engineered point spread function (PSF). Traditional approaches used in microscopic super-resolution imaging such as the Double-Helix PSF (DHPSF) are…

Image and Video Processing · Electrical Eng. & Systems 2022-08-05 Bhargav Ghanekar , Vishwanath Saragadam , Dushyant Mehra , Anna-Karin Gustavsson , Aswin Sankaranarayanan , Ashok Veeraraghavan

We develop a speckle-tracking method for x-ray phase-contrast imaging, based on the concept of geometric flow. This flow is a conserved current associated with deformation of illuminating x-ray speckles induced by passage through a sample.…

Medical Physics · Physics 2018-11-14 David M. Paganin , Helene Labriet , Emmanuel Brun , Sebastien Berujon

With the advance of fluorescence imaging technologies, recently cell biologists are able to record the movement of protein vesicles within a living cell. Automatic tracking of the movements of these vesicles become key for qualitative…

Quantitative Methods · Quantitative Biology 2015-06-09 Min Xu

This article describes two independent developments aimed at improving the Particle Tracking Method for measurements of flow or particle velocities. First, a stereoscopic multicamera calibration method that does not require any optical…

Methods to extract information from the tracking of mobile objects/particles have broad interest in biological and physical sciences. Techniques based on simple criteria of proximity in time-consecutive snapshots are useful to identify the…

Data Analysis, Statistics and Probability · Physics 2015-03-13 M. Chertkov , L. Kroc , F. Krzakala , M. Vergassola , L. Zdeborová

Multi-target tracking is an important problem in civilian and military applications. This paper investigates multi-target tracking in distributed sensor networks. Data association, which arises particularly in multi-object scenarios, can be…

Multiagent Systems · Computer Science 2018-12-04 Mark R. Leonard , Abdelhak M. Zoubir

Efficient monitoring of airborne particulate matter (PM), especially particles with aerodynamic diameter less than 2.5 um (PM2.5), is crucial for improving public health. Reliable information on the concentration, size distribution and…

Applied Physics · Physics 2020-01-08 Abhinav Sharma , Shangran Xie , Richard Zeltner , Philip St. J. Russell

Due to its rising importance in science and technology in recent years, particle tracking in videos presents itself as a tool for successfully acquiring new knowledge in the field of life sciences and physics. Accordingly, different…

Data Analysis, Statistics and Probability · Physics 2021-02-01 Kristina Dingel , Rico Huhnstock , André Knie , Arno Ehresmann , Bernhard Sick

Tracking many cells in time-lapse 3D image sequences is an important challenging task of bioimage informatics. Motivated by a study of brain-wide 4D imaging of neural activity in C. elegans, we present a new method of multi-cell tracking.…

Computer Vision and Pattern Recognition · Computer Science 2018-01-18 Osamu Hirose , Shotaro Kawaguchi , Terumasa Tokunaga , Yu Toyoshima , Takayuki Teramoto , Sayuri Kuge , Takeshi Ishihara , Yuichi Iino , Ryo Yoshida

The development of robust, real-time optical methods for the detection and tracking of particles in complex multiple scattering media is a problem of practical importance in a number of fields, including environmental monitoring, air…

Optics · Physics 2025-02-04 Mohammadrasoul Taghavi , Edwin A. Marengo

The major challenge to improve deterministic single ion sources is to control the position and momentum of each ion. Based on the extra information given by the electron created in a photoionization process, the trajectory of the correlated…

Instrumentation and Detectors · Physics 2019-07-16 C. Lopez , A. Trimeche , D. Comparat , Y. J. Picard

A method for particle orientation tracking is developed and demonstrated specifically for anisotropic particles. Using (high-speed) multi-camera recordings of anisotropic particles from different viewpoints, we reconstruct the 3D location…

Computer Vision and Pattern Recognition · Computer Science 2025-05-13 Mees M. Flapper , Elian Bernard , Sander G. Huisman

Radially self-accelerating light exhibits an intensity pattern that describes a spiraling trajectory around the optical axis as the beam propagates. In this article, we show in simulation and experiment how such beams can be used to perform…

Optics · Physics 2021-04-08 Shashi Prabhakar , Stephen Plachta , Marco Ornigotti , Robert Fickler

Time course measurement of single molecules on a cell surface provides detailed information on the dynamics of the molecules, which is otherwise inaccessible. To extract the quantitative information, single particle tracking (SPT) is…

Quantitative Methods · Quantitative Biology 2016-01-06 Shunsuke Teraguchi , Yutaro Kumagai

Particle flows of high particle concentration are important in many fields, including chemical processing, pharmaceutical processing, energy conversion and powder transport. However, despite decades of research and industrial application,…

Fluid Dynamics · Physics 2013-11-06 Frank Shaffer , Balaji Gopalan

Particle-laden flows occur in a wide range of disciplines, from atmospheric flows to renewable energy to turbomachinery. They generally pose a challenging environment for the numerical prediction of particle-induced phenomena due to their…

Computational Physics · Physics 2023-05-17 Patrick Kopper , Anna Schwarz , Stephen M. Copplestone , Philip Ortwein , Stephan Staudacher , Andrea Beck

Single-particle tracking (SPT) grants unprecedented insight into cellular function at the molecular scale [1]. Throughout the cell, the movement of single-molecules is generally heterogeneous and complex. Hence, there is an imperative to…

Quantitative Methods · Quantitative Biology 2015-06-24 Mohamed El Beheiry , Maxime Dahan , Jean-Baptiste Masson

Single particle tracking is essential in many branches of science and technology, from the measurement of biomolecular forces to the study of colloidal crystals. Standard current methods rely on algorithmic approaches: by fine-tuning…

Soft Condensed Matter · Physics 2018-12-07 Saga Helgadottir , Aykut Argun , Giovanni Volpe

When a binary fluid demixes under a slow temperature ramp, nucleation, coarsening and sedimentation of droplets lead to an oscillatory evolution of the phase separating system. The advection of the sedimenting droplets is found to be…

Fluid Dynamics · Physics 2012-05-31 Tobias Lapp , Martin Rohloff , Jürgen Vollmer , Björn Hof