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A critical challenge in single-cell RNA sequencing (scRNA-seq) integration is resolving the tension between eliminating batch effects and maintaining biological fidelity. While recent evidence indicates that batch effects manifest…

Machine Learning · Computer Science 2026-05-19 Xichen Yan , Zelin Zang , Changxi Chi , Jingbo Zhou , Chang Yu , Jinlin Wu , Shenghui Cheng , Fuji Yang , Jiebo Luo , Zhen Lei , Stan Z. Li

Background: High-throughput techniques bring novel tools but also statistical challenges to genomic research. Identifying genes with differential expression between different species is an effective way to discover evolutionarily conserved…

Methodology · Statistics 2018-10-05 Yan Zhou , Jiadi Zhu , Tiejun Tong , Junhui Wang , Bingqing Lin , Jun Zhang

scRNA-seq clustering is a critical task for analyzing single-cell RNA sequencing (scRNA-seq) data, as it groups cells with similar gene expression profiles. Transformers, as powerful foundational models, have been applied to scRNA-seq…

Machine Learning · Computer Science 2026-02-10 Zhuomin Liang , Liang Bai , Xian Yang

LLM development has aroused great interest in Sequential Recommendation (SR) applications. However, comprehensive evaluation of SR models remains lacking due to the limitations of the existing benchmarks: 1) an overemphasis on accuracy,…

Information Retrieval · Computer Science 2026-04-14 Jianhong Li , Zeheng Qian , Wangze Ni , Haoyang Li , Hongwei Yao , Yang Bai , Kui Ren

The existence of doublets is a key confounder in single-cell RNA sequencing (scRNA-seq) data analysis. Computational methods have been developed for detecting doublets from scRNA-seq data. We developed an R package DoubletCollection to…

Genomics · Quantitative Biology 2021-12-01 Nan Miles Xi , Jingyi Jessica Li

Late diagnosis and high costs are key factors that negatively impact the care of cancer patients worldwide. Although the availability of biological markers for the diagnosis of cancer type is increasing, costs and reliability of tests…

Machine Learning · Computer Science 2019-08-20 Sterling Ramroach , Melford John , Ajay Joshi

Single-cell RNA sequencing (scRNA-seq) enables transcriptomic profiling at cellular resolution but suffers from pervasive dropout events that obscure biological signals. We present SCR-MF, a modular two-stage workflow that combines…

Machine Learning · Computer Science 2025-11-24 Ali Anaissi , Deshao Liu , Yuanzhe Jia , Weidong Huang , Widad Alyassine , Junaid Akram

On June 25th, 2018, Huang et al. published a computational method SAVER on Nature Methods for imputing dropout gene expression levels in single cell RNA sequencing (scRNA-seq) data. Huang et al. performed a set of comprehensive benchmarking…

Applications · Statistics 2019-08-21 Wei Vivian Li , Jingyi Jessica Li

In single-cell RNA sequencing (scRNA-seq) analysis, a key challenge is inferring hidden cellular dynamics from static cell snapshots. Various computational methods have been developed to address this, focusing on perspectives like…

Genomics · Quantitative Biology 2024-09-04 Qingyang Wang , Zhiqian Zhai , Qiuyu Lian , Dongyuan Song , Jingyi Jessica Li

Single-cell RNA-sequencing (scRNA-seq) stands as a powerful tool for deciphering cellular heterogeneity and exploring gene expression profiles at high resolution. However, its high cost renders it impractical for extensive sample cohorts…

Causal inference is a vital aspect of multiple scientific disciplines and is routinely applied to high-impact applications such as medicine. However, evaluating the performance of causal inference methods in real-world environments is…

Machine Learning · Computer Science 2023-07-04 Mathieu Chevalley , Yusuf Roohani , Arash Mehrjou , Jure Leskovec , Patrick Schwab

In cancer research, clustering techniques are widely used for exploratory analyses and dimensionality reduction, playing a critical role in the identification of novel cancer subtypes, often with direct implications for patient management.…

Proteins perform critical processes in all living systems: converting solar energy into chemical energy, replicating DNA, as the basis of highly performant materials, sensing and much more. While an incredible range of functionality has…

Biomolecules · Quantitative Biology 2021-09-29 Leonardo V. Castorina , Rokas Petrenas , Kartic Subr , Christopher W. Wood

The use of deep learning models in computational biology has increased massively in recent years, and it is expected to continue with the current advances in the fields such as Natural Language Processing. These models, although able to…

Motivated by theoretical advancements in dimensionality reduction techniques we use a recent model, called Block Markov Chains, to conduct a practical study of clustering in real-world sequential data. Clustering algorithms for Block Markov…

Machine Learning · Computer Science 2022-10-05 Alexander Van Werde , Albert Senen-Cerda , Gianluca Kosmella , Jaron Sanders

Finding meaningful groups, i.e., clusters, in high-dimensional data such as images or texts without labeled data at hand is an important challenge in data mining. In recent years, deep clustering methods have achieved remarkable results in…

Machine Learning · Computer Science 2024-10-15 Collin Leiber , Niklas Strauß , Matthias Schubert , Thomas Seidl

The existence of doublets in single-cell RNA sequencing (scRNA-seq) data poses a great challenge in downstream data analysis. Computational doublet-detection methods have been developed to remove doublets from scRNA-seq data. Yet, the…

Quantitative Methods · Quantitative Biology 2023-02-07 Nan Miles Xi , Angelos Vasilopoulos

Computational complexity is a key limitation of genomic analyses. Thus, over the last 30 years, researchers have proposed numerous fast heuristic methods that provide computational relief. Comparing genomic sequences is one of the most…

Clustering is an unsupervised machine learning methodology where unlabeled elements/objects are grouped together aiming to the construction of well-established clusters that their elements are classified according to their similarity. The…

Machine Learning · Statistics 2023-10-20 Dimitrios Saligkaras , Vasileios E. Papageorgiou

As a powerful tool for characterizing cellular subpopulations and cellular heterogeneity, single cell RNA sequencing (scRNA-seq) technology offers advantages of high throughput and multidimensional analysis. However, the process of data…

Machine Learning · Computer Science 2024-11-19 Zhuorui Cui , Shengze Dong , Ding Liu