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Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

Optics · Physics 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

When it comes to deploying deep vision models, the behavior of these systems must be explicable to ensure confidence in their reliability and fairness. A common approach to evaluate deep learning models is to build a labeled test set with…

Computer Vision and Pattern Recognition · Computer Science 2023-03-28 Jinqi Luo , Zhaoning Wang , Chen Henry Wu , Dong Huang , Fernando De la Torre

We demonstrate a deep learning-based offline autofocusing method, termed Deep-R, that is trained to rapidly and blindly autofocus a single-shot microscopy image of a specimen that is acquired at an arbitrary out-of-focus plane. We…

Image and Video Processing · Electrical Eng. & Systems 2021-01-25 Yilin Luo , Luzhe Huang , Yair Rivenson , Aydogan Ozcan

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

Quantitative Methods · Quantitative Biology 2025-11-03 Surajit Das , Pavel Zun

Autofocus (AF) methods are extensively used in biomicroscopy, for example to acquire timelapses, where the imaged objects tend to drift out of focus. AD algorithms determine an optimal distance by which to move the sample back into the…

Image and Video Processing · Electrical Eng. & Systems 2021-01-14 Adrian Shajkofci , Michael Liebling

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

Whole slide imaging (WSI) has recently been cleared for primary diagnosis in the US. A critical challenge of WSI is to perform accurate focusing in high speed. Traditional systems create a focus map prior to scanning. For each focus point…

Computer Vision and Pattern Recognition · Computer Science 2019-04-26 Jun Liao , Yutong Jiang , Zichao Bian , Bahareh Mahrou , Aparna Nambiar , Alexander W. Magsam , Kaikai Guo , Yong Ku Cho , Guoan Zheng

Lensless optical imaging eliminates the need for refractive optics, enabling compact and low-cost cameras with a large field-of-view, supporting point-of-care diagnostics and industrial monitoring. Practical deployments, however, remain…

Large-scale pretrained vision-language models like CLIP have demonstrated remarkable zero-shot image classification capabilities across diverse domains. To enhance CLIP's performance while preserving the zero-shot paradigm, various…

Computer Vision and Pattern Recognition · Computer Science 2024-06-19 Xuefeng Hu , Ke Zhang , Min Sun , Albert Chen , Cheng-Hao Kuo , Ram Nevatia

Zero-shot and prompt-based models have excelled at visual reasoning tasks by leveraging large-scale natural image corpora, but they often fail on sparse and domain-specific scientific image data. We introduce Zenesis, a no-code interactive…

Computer Vision and Pattern Recognition · Computer Science 2025-08-19 Shubhabrata Mukherjee , Jack Lang , Obeen Kwon , Iryna Zenyuk , Valerie Brogden , Adam Weber , Daniela Ushizima

We present a rapid, large-field bimodal imaging platform that integrates conventional brightfield microscopy with a lensless IR imaging scanner, enabling whole-slide IR image stack acquisition in minutes. Using a dedicated deep learning…

Brillouin microscopy is rapidly emerging as a powerful technique for imaging the mechanical properties of biological specimens in a label-free, non-contact manner. We present a standardized file format and open-source tools to facilitate…

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

Noninvasive optical imaging modalities can probe patient's tissue in 3D and over time generate gigabytes of clinically relevant data per sample. There is a need for AI models to analyze this data and assist clinical workflow. The lack of…

Zero-shot anomaly detection (ZSAD) offers potential for identifying anomalies in medical imaging without task-specific training. In this paper, we evaluate CLIP-based models, originally developed for industrial tasks, on brain tumor…

Computer Vision and Pattern Recognition · Computer Science 2024-11-15 Aldo Marzullo , Marta Bianca Maria Ranzini

One of the challenges facing the adoption of digital pathology workflows for clinical use is the need for automated quality control. As the scanners sometimes determine focus inaccurately, the resultant image blur deteriorates the scanned…

Image and Video Processing · Electrical Eng. & Systems 2018-11-16 Mahdi S. Hosseini , Yueyang Zhang , Lyndon Chan , Konstantinos N. Plataniotis , Jasper A. Z. Brawley-Hayes , Savvas Damaskinos

The lensless endoscope is a promising device designed to image tissues in vivo at the cellular scale. The traditional acquisition setup consists in raster scanning during which the focused light beam from the optical fiber illuminates…

Computer Vision and Pattern Recognition · Computer Science 2018-10-31 Stéphanie Guérit , Siddharth Sivankutty , Camille Scotté , John Alto Lee , Hervé Rigneault , Laurent Jacques

Live time-lapse microscopy is essential for a wide range of biological applications. Software-based automation is the gold standard for the operation of hardware accessories necessary for image acquisition. Given that current software…

Image and Video Processing · Electrical Eng. & Systems 2019-06-20 Quang Long Pham , David Chege , Timothy Dijamco , Migle Surblyte , Akshay Naik , Kolawole Campbell , Nhat-Anh-Nguyen Tong , Roman Voronov

In this report we present an unsupervised image registration framework, using a pre-trained deep neural network as a feature extractor. We refer this to zero-shot learning, due to nonoverlap between training and testing dataset (none of the…

Computer Vision and Pattern Recognition · Computer Science 2019-08-20 Avinash Kori , Ganapathi Krishnamurthi

The effectiveness of zero-shot classification in large vision-language models (VLMs), such as Contrastive Language-Image Pre-training (CLIP), depends on access to extensive, well-aligned text-image datasets. In this work, we introduce two…

Computer Vision and Pattern Recognition · Computer Science 2025-02-27 Anju Rani , Daniel O. Arroyo , Petar Durdevic
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