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Traditional deep learning-based methods for classifying cellular features in microscopy images require time- and labor-intensive processes for training models. Among the current limitations are major time commitments from domain experts for…

Image and Video Processing · Electrical Eng. & Systems 2025-01-22 Abhiram Kandiyana , Peter R. Mouton , Yaroslav Kolinko , Lawrence O. Hall , Dmitry Goldgof

Image segmentation is a fundamental problem in biomedical image analysis. Recent advances in deep learning have achieved promising results on many biomedical image segmentation benchmarks. However, due to large variations in biomedical…

Computer Vision and Pattern Recognition · Computer Science 2017-06-16 Lin Yang , Yizhe Zhang , Jianxu Chen , Siyuan Zhang , Danny Z. Chen

Developing clinically useful cell-level analysis tools in digital pathology remains challenging due to limitations in dataset granularity, inconsistent annotations, high computational demands, and difficulties integrating new technologies…

Computer Vision and Pattern Recognition · Computer Science 2025-12-02 Nikita Shvetsov , Thomas K. Kilvaer , Masoud Tafavvoghi , Anders Sildnes , Kajsa Møllersen , Lill-Tove Rasmussen Busund , Lars Ailo Bongo

We consider the problem of accurately identifying cell boundaries and labeling individual cells in confocal microscopy images, specifically, 3D image stacks of cells with tagged cell membranes. Precise identification of cell boundaries,…

Computer Vision and Pattern Recognition · Computer Science 2019-09-06 Jiaxiang Jiang , Po-Yu Kao , Samuel A. Belteton , Daniel B. Szymanski , B. S. Manjunath

Automated and semi-automated techniques in biomedical electron microscopy (EM) enable the acquisition of large datasets at a high rate. Segmentation methods are therefore essential to analyze and interpret these large volumes of data, which…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Anusha Aswath , Ahmad Alsahaf , Ben N. G. Giepmans , George Azzopardi

Accurately segmenting and individualizing cells in SEM images is a highly promising technique for elucidating tissue architecture in oncology. While current AI-based methods are effective, errors persist, necessitating time-consuming manual…

Image and Video Processing · Electrical Eng. & Systems 2025-04-11 Florian Robert , Alexia Calovoulos , Laurent Facq , Fanny Decoeur , Etienne Gontier , Christophe F. Grosset , Baudouin Denis de Senneville

Obtaining large-scale medical data, annotated or unannotated, is challenging due to stringent privacy regulations and data protection policies. In addition, annotating medical images requires that domain experts manually delineate…

Computer Vision and Pattern Recognition · Computer Science 2025-05-16 Tushar Kataria , Shireen Y. Elhabian

Large, high-quality, annotated datasets are the foundation of medical AI research, but constructing even a small, moderate-quality, annotated dataset can take years of effort from multidisciplinary teams. Although active learning can…

Digital PCR (dPCR) has revolutionized nucleic acid diagnostics by enabling absolute quantification of rare mutations and target sequences. However, current detection methodologies face challenges, as flow cytometers are costly and complex,…

Quantitative Methods · Quantitative Biology 2024-03-29 Yuanyuan Wei , Shanhang Luo , Changran Xu , Yingqi Fu , Qingyue Dong , Yi Zhang , Fuyang Qu , Guangyao Cheng , Yi-Ping Ho , Ho-Pui Ho , Wu Yuan

The accurate segmentation and tracking of cells in microscopy image sequences is an important task in biomedical research, e.g., for studying the development of tissues, organs or entire organisms. However, the segmentation of touching…

Computer Vision and Pattern Recognition · Computer Science 2020-12-10 Tim Scherr , Katharina Löffler , Moritz Böhland , Ralf Mikut

The application of machine learning techniques to the medical domain is especially challenging due to the required level of precision and the incurrence of huge risks of minute errors. Employing these techniques to a more complex subdomain…

Computer Vision and Pattern Recognition · Computer Science 2021-10-19 Ekta Gavas , Kaustubh Olpadkar

Pathologists routinely alternate between different magnifications when examining Whole-Slide Images, allowing them to evaluate both broad tissue morphology and intricate cellular details to form comprehensive diagnoses. However, existing…

Medical image segmentation is an important analysis task in clinical practice and research. Deep learning has massively advanced the field, but current approaches are mostly based on models trained for a specific task. Training such models…

Image and Video Processing · Electrical Eng. & Systems 2025-12-18 Anwai Archit , Luca Freckmann , Constantin Pape

In digital pathology, cell detection and classification are often prerequisites to quantify cell abundance and explore tissue spatial heterogeneity. However, these tasks are particularly challenging for multiplex immunohistochemistry (mIHC)…

Image and Video Processing · Electrical Eng. & Systems 2019-08-05 Yeman Brhane Hagos , Priya Lakshmi Narayanan , Ayse U. Akarca , Teresa Marafioti , Yinyin Yuan

We present a scalable method for brain cell identification in multiview confocal light sheet microscopy images. Our algorithmic pipeline includes a hierarchical registration approach and a novel multiview version of semantic deconvolution…

Computer Vision and Pattern Recognition · Computer Science 2015-11-05 Marco Paciscopi , Ludovico Silvestri , Francesco Saverio Pavone , Paolo Frasconi

Accurate segmentation and classification of brain tumors from Magnetic Resonance Imaging (MRI) remain key challenges in medical image analysis, primarily due to the lack of high-quality, balanced, and diverse datasets with expert…

Image and Video Processing · Electrical Eng. & Systems 2026-01-29 Amirreza Fateh , Yasin Rezvani , Sara Moayedi , Sadjad Rezvani , Fatemeh Fateh , Mansoor Fateh , Vahid Abolghasemi

We introduce a new AI-ready computational pathology dataset containing restained and co-registered digitized images from eight head-and-neck squamous cell carcinoma patients. Specifically, the same tumor sections were stained with the…

Image and Video Processing · Electrical Eng. & Systems 2023-05-29 Parmida Ghahremani , Joseph Marino , Juan Hernandez-Prera , Janis V. de la Iglesia , Robbert JC Slebos , Christine H. Chung , Saad Nadeem

Medical image annotation is essential for diagnosing diseases, yet manual annotation is time-consuming, costly, and prone to variability among experts. To address these challenges, we propose an automated explainable annotation system that…

Computer Vision and Pattern Recognition · Computer Science 2025-02-26 Basma Nasir , Tehseen Zia , Muhammad Nawaz , Catarina Moreira

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai
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