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Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Surface-enhanced Raman spectroscopy (SERS) allows single-molecule detection due to the strong field localization occurring at sharp bends or kinks of the metal-vacuum interface. An important question concerns the limits of the signal…

Optics · Physics 2008-06-19 Sanshui Xiao , Niels Asger Mortensen , Antti-Pekka Jauho

We report the first implementation of laser scanning Coherent Stokes Raman scattering (CSRS - pronounced "sCiSsoRS") microscopy. To overcome the major challenge in CSRS imaging, we show how to suppress the fluorescence background by narrow…

Optics · Physics 2023-06-21 Sandro Heuke , Hervé Rigneault

A miniaturized full-frequency encoded illumination (mini-FEI) chip is presented for high-throughput super-resolution imaging using the spatial frequency shift (SFS) effect. A tunable full SFS scheme is achieved through propagating and…

Waveguide-enhanced Raman spectroscopy (WERS) is a promising technique for sensitive and selective detection of chemicals in a compact chip-scale platform. Coupling light on and off the sensor chip with fibers however presents challenges…

Applied Physics · Physics 2020-05-05 Derek M. Kita , Jérôme Michon , Juejun Hu

Histological examination is crucial for cancer diagnosis, including hematoxylin and eosin (H&E) staining for mapping morphology and immunohistochemistry (IHC) staining for revealing chemical information. Recently developed two-color…

Tissues and Organs · Quantitative Biology 2023-09-22 Hongli Ni , Chinmayee Prabhu Dessai , Haonan Lin , Wei Wang , Shaoxiong Chen , Yuhao Yuan , Xiaowei Ge , Jianpeng Ao , Nolan Vild , Ji-Xin Cheng

The ability to identify the contents of a sealed container, without the need to extract a sample, is desirable in applications ranging from forensics to product quality control. One technique suited to this is inverse spatially offset Raman…

More than twenty years ago, scientists succeeded in pushing the limits of optical detection to single molecules using fluorescence. This breakthrough has revolutionized biophysical measurements, but restrictions in photophysics and labeling…

Biological Physics · Physics 2015-06-17 Marek Piliarik , Vahid Sandoghdar

Infrared (IR) imaging is commonly used in various scenarios, including autonomous driving, fire safety and defense applications. Thus, semantic segmentation of such images is of great interest. However, this task faces several challenges,…

Computer Vision and Pattern Recognition · Computer Science 2024-12-10 Junno Yun , Mehmet Akçakaya

A novel technique was demonstrated that overcome important drawbacks to crosslink cells by irradiation with ultrashort UV laser pulses (L-crosslinking). To use this technique coupled to Chromatin ImmunoPrecipitation (ChIP) in a high…

In optical communications, the Raman effect is exploited for its lasing properties in distributed Raman amplification (DRA) and leads to spectral distortions through inter-channel stimulated Raman scattering (ISRS). In single-mode fibers,…

Optics · Physics 2026-04-13 Lucas Alves Zischler , Antonio Mecozzi , Cristian Antonelli

Surface enhanced Raman scattering (SERS) exploits surface plasmons induced by the incident field in metallic nanostructures to significantly increase the Raman intensity. Graphene provides the ideal prototype two dimensional (2d) test…

Mesoscale and Nanoscale Physics · Physics 2013-04-03 F. Schedin , E. Lidorikis , A. Lombardo , V. G. Kravets , A. K. Geim , A. N. Grigorenko , K. S. Novoselov , A. C. Ferrari

Raman-based technologies have enabled many ground-breaking scientific discoveries related to surface science, single molecule chemistry and biology. For example, researchers have identified surface bound molecules by their Raman vibrational…

Optics · Physics 2020-03-12 Xiaoqin Shen , Hyungwoo Choi , Dongyu Chen , Wei Zhao , Andrea M. Armani

Stimulated Raman scattering is a well-known nonlinear process that can be harnessed to produce optical gain in a wide variety of media. This effect has been used to produce the first silicon-based lasers and high-gain amplifiers.…

Optics · Physics 2008-10-13 D. R. Solli , P. Koonath , B. Jalali

Raman scattering signal can be enhanced through localization of incident field into sub-wavelength hot-spots through plasmonic nano-structures (Surface-enhanced Raman scattering-SERS). Recently, further enhancement of SERS signal via…

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

Photonics caught world attention since channel capacity limit of metallic interconnects approached due to research and design in high speed digital processors. Use of dielectrics, instead, suitable for light propagation was more attractive…

Optics · Physics 2024-12-04 Abdurrahman Javid Shaikh , Othman Sidek

Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive and selective technique that greatly enhances the signal of an analyte, compared with its signal from classical Raman Spectroscopy, due to its interaction with a substrates…

Materials Science · Physics 2025-10-20 Jan Komeda , Antonio Cammarata , Tomas Polcar

Due to its particular geometry, stimulated Raman side-scattering (SRSS) drives scattered light emission at non-conventional directions, leading to scarce and complex experimental observations. Direct-irradiation campaigns at the SG-II UP…

A Gaussian noise (GN) model is presented that properly accounts for an arbitrary frequency dependent signal power profile along the link. This enables the evaluation of the impact of inter-channel stimulated Raman scattering (ISRS) on the…

Signal Processing · Electrical Eng. & Systems 2018-07-04 Daniel Semrau , Polina Bayvel
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