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In digital pathology, different staining procedures and scanners cause substantial color variations in whole-slide images (WSIs), especially across different laboratories. These color shifts result in a poor generalization of deep…

Image and Video Processing · Electrical Eng. & Systems 2021-07-27 Sophia J. Wagner , Nadieh Khalili , Raghav Sharma , Melanie Boxberg , Carsten Marr , Walter de Back , Tingying Peng

Hematoxylin and Eosin (H&E) has been the gold standard in tissue analysis for decades, however, tissue specimens stained in different laboratories vary, often significantly, in appearance. This variation poses a challenge for both…

Image and Video Processing · Electrical Eng. & Systems 2025-06-25 Umair Khan , Jouni Härkönen , Marjukka Friman , Leena Latonen , Teijo Kuopio , Pekka Ruusuvuori

Registration of serial whole-slide histopathological images (WSIs) is critical for enabling direct comparison across diverse stains and for preparing paired datasets in artificial intelligence (AI) workflows such as virtual staining and…

Computer Vision and Pattern Recognition · Computer Science 2025-09-04 Zeyu Liu , Shengwei Ding

The automatic registration of noninvasive second-harmonic generation microscopy to hematoxylin and eosin slides is a highly desired, yet still unsolved problem. The task is challenging because the second-harmonic images contain only partial…

Computer Vision and Pattern Recognition · Computer Science 2024-09-25 Marek Wodzinski , Henning Müller

We developed a pipeline for registering pre-surgery Magnetic Resonance (MR) images and post-resection Ultrasound (US) images. Our approach leverages unpaired style transfer using 3D CycleGAN to generate synthetic T1 images, thereby…

Image and Video Processing · Electrical Eng. & Systems 2025-08-08 Junyi Wang , Xi Zhu , Yikun Guo , Zixi Wang , Haichuan Gao , Le Zhang , Fan Zhang

Stain color variation in histological images, caused by a variety of factors, is a challenge not only for the visual diagnosis of pathologists but also for cell segmentation algorithms. To eliminate the color variation, many stain…

Image and Video Processing · Electrical Eng. & Systems 2022-10-27 Huaqian Wu , Nicolas Souedet , Camille Mabillon , Caroline Jan , Cédric Clouchoux , Thierry Delzescaux

Image registration (IR) is a process that deforms images to align them with respect to a reference space, making it easier for medical practitioners to examine various medical images in a standardized reference frame, such as having the…

Image and Video Processing · Electrical Eng. & Systems 2024-01-11 Ahmad Hammoudeh , Stéphane Dupont

Virtual staining of histopathology images (e.g., H&E-IHC) is an emerging tool in digital pathology, enabling faster and cheaper workflows by synthesizing target stains from routinely acquired slides. Yet, the quality of virtual staining…

Computer Vision and Pattern Recognition · Computer Science 2026-05-21 Fedor Gubanov , Svetlana Illarionova , Vlad Kozlovskiy , Mikhail Romanov , Yersultan Akhmetov , Aida Akaeva , Vyacheslav Grinevich , Rifat Hamoudi , Maxim Sharaev

Performance of deep learning algorithms decreases drastically if the data distributions of the training and testing sets are different. Due to variations in staining protocols, reagent brands, and habits of technicians, color variation in…

Computer Vision and Pattern Recognition · Computer Science 2020-12-01 Abhijeet Patil , Mohd. Talha , Aniket Bhatia , Nikhil Cherian Kurian , Sammed Mangale , Sunil Patel , Amit Sethi

Digitized Histological diagnosis is in increasing demand. However, color variations due to various factors are imposing obstacles to the diagnosis process. The problem of stain color variations is a well-defined problem with many proposed…

Computer Vision and Pattern Recognition · Computer Science 2018-04-06 M Tarek Shaban , Christoph Baur , Nassir Navab , Shadi Albarqouni

Three-dimensional X-ray histology techniques offer a non-invasive alternative to conventional 2D histology, enabling volumetric imaging of biological tissues without the need for physical sectioning or chemical staining. However, the…

Image and Video Processing · Electrical Eng. & Systems 2025-09-12 Sarah C. Irvine , Christian Lucas , Diana Krüger , Bianca Guedert , Julian Moosmann , Berit Zeller-Plumhoff

The variation in histologic staining between different medical centers is one of the most profound challenges in the field of computer-aided diagnosis. The appearance disparity of pathological whole slide images causes algorithms to become…

Image and Video Processing · Electrical Eng. & Systems 2024-04-05 Martin J. Hetz , Tabea-Clara Bucher , Titus J. Brinker

Histopathology image analysis is fundamental to digital pathology, with hematoxylin and eosin (H&E) staining as the gold standard for diagnostic and prognostic assessments. While H&E imaging effectively highlights cellular and tissue…

Image and Video Processing · Electrical Eng. & Systems 2025-11-18 Yao Du , Jiaxin Zhuang , Xiaoyu Zheng , Jing Cong , Limei Guo , Chao He , Lin Luo , Xiaomeng Li

Acquisition-to-acquisition signal intensity variations (non-standardness) are inherent in MR images. Standardization is a post processing method for correcting inter-subject intensity variations through transforming all images from the…

Computer Vision and Pattern Recognition · Computer Science 2015-05-18 Ulas Bagci , Jayaram K. Udupa , Li Bai

The correlation of optical measurements with a correct pathology label is often hampered by imprecise registration caused by deformations in histology images. This study explores an automated multi-modal image registration technique…

Image and Video Processing · Electrical Eng. & Systems 2023-11-27 Lianne Feenstra , Maud Lambregts , Theo J. M Ruers , Behdad Dashtbozorg

Pathologists need to combine information from differently stained pathology slices for accurate diagnosis. Deformable image registration is a necessary technique for fusing multi-modal pathology slices. This paper proposes a hybrid deep…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Chulong Zhang , Yuming Jiang , Na Li , Zhicheng Zhang , Md Tauhidul Islam , Jingjing Dai , Lin Liu , Wenfeng He , Wenjian Qin , Jing Xiong , Yaoqin Xie , Xiaokun Liang

The process of digitising histology slides involves multiple factors that can affect a whole slide image's (WSI) final appearance, including the staining protocol, scanner, and tissue type. This variability constitutes a domain shift and…

Computer Vision and Pattern Recognition · Computer Science 2024-11-26 Manahil Raza , Saad Bashir , Talha Qaiser , Nasir Rajpoot

The PI-CAI (Prostate Imaging: Cancer AI) challenge led to expert-level diagnostic algorithms for clinically significant prostate cancer detection. The algorithms receive biparametric MRI scans as input, which consist of T2-weighted and…

Image and Video Processing · Electrical Eng. & Systems 2024-07-01 Alessa Hering , Sarah de Boer , Anindo Saha , Jasper J. Twilt , Mattias P. Heinrich , Derya Yakar , Maarten de Rooij , Henkjan Huisman , Joeran S. Bosma

Double staining in histopathology, particularly for metaplastic breast cancer, typically employs H&E and P63 dyes. However, P63's tissue damage and high cost necessitate alternative methods. This study introduces xAI-CycleGAN, an advanced…

Image and Video Processing · Electrical Eng. & Systems 2023-12-07 Tibor Sloboda , Lukáš Hudec , Wanda Benešová

Computational histopathology image diagnosis becomes increasingly popular and important, where images are segmented or classified for disease diagnosis by computers. While pathologists do not struggle with color variations in slides,…

Image and Video Processing · Electrical Eng. & Systems 2020-07-27 Hanwen Liang , Konstantinos N. Plataniotis , Xingyu Li
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