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Related papers: Associative and Segregative Liquid-Liquid Phase Se…

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Living and engineered systems rely on the stable coexistence of two interspersed liquid phases. Yet surface tension drives their complete separation. Here we show that stable droplets of uniform and tuneable size can be produced through…

The assembly of functional biomolecular condensates often involves liquid-liquid phase separation (LLPS) of proteins with multiple modular domains, which can be folded or conformationally disordered to various degrees. To understand the…

Biomolecules · Quantitative Biology 2022-01-11 Yi-Hsuan Lin , Haowei Wu , Bowen Jia , Mingjie Zhang , Hue Sun Chan

In view of the notorious complexity of protein--protein interactions, simplified models of proteins treated as patchy particles offer a promising strategy to obtain insight into the mechanism of crystallization. Here we report…

Soft Condensed Matter · Physics 2019-10-16 Jens Glaser , Sharon C. Glotzer

The precise control of liquid-liquid phase separation (LLPS) is the key to developing cutting-edge technologies that benefit diverse disciplines. Fluid flow was found to be capable of controlling the structure and effective temperature of…

Fluid Dynamics · Physics 2024-07-04 Yulin Li , Tong Zhou , Yanyu Li , Qi Zhang , Zhihong You

Membraneless droplets or liquid condensates formed via liquid-liquid phase separation (LLPS) play a pivotal role in cell biology and hold potential for biomedical engineering. While membraneless droplets are often studied in the context of…

Soft Condensed Matter · Physics 2025-11-07 Chenxi Liu , Ding Cao , Siyu Liu , Yilin Wu

Globular proteins as well as recently synthesized colloids engineered with differently charged surface regions have in common a reduced bonding valence and a complex interaction pattern dominated by like-charge attraction and…

Soft Condensed Matter · Physics 2024-02-02 Daniele Notarmuzi , Emanuela Bianchi

Biomolecular condensates such as membraneless organelles, underpinned by liquid-liquid phase separation (LLPS), are important for physiological function, with electrostatics -- among other interaction types -- being a prominent force in…

Biomolecules · Quantitative Biology 2021-05-26 Jonas Wessén , Tanmoy Pal , Suman Das , Yi-Hsuan Lin , Hue Sun Chan

Liquid-liquid phase separation of aqueous two-phase system (ATPS) is fundamental across physical and biological sciences. While well understood for passive systems, how this process is regulated by active agents such as motile bacteria…

Soft Condensed Matter · Physics 2025-11-25 Dixi Yang , Anheng Wang , Chunming Wang , Hajime Tanaka , Jiaxing Yuan

Liquid-liquid phase separation (LLPS) involving intrinsically disordered protein regions (IDRs) is a major physical mechanism for biological membraneless compartmentalization. The multifaceted electrostatic effects in these biomolecular…

Water is an unique material with a long list of thermodynamic, dynamic and structural anomalies, which are usually attributed to the competition between two characteristic length scales in the intermolecular interaction. It has been argued…

Statistical Mechanics · Physics 2021-06-25 Murilo S. Marques , Vinicius Fonseca Hernandes , Enrique Lomba , J. Rafael Bordin

Employing X-ray photon correlation spectroscopy we measure the kinetics and dynamics of a pressure-induced liquid-liquid phase separation (LLPS) in a water-lysozyme solution. Scattering invariants and kinetic information provide evidence…

Soft Condensed Matter · Physics 2021-07-13 M. Moron , A. Al-Masoodi , C. Lovato , M. Reiser , L. Randolph , G. Surmeier , J. Bolle , F. Westermeier , M. Sprung , R. Winter , M. Paulus , C. Gutt

Liquid-liquid phase separation (LLPS) is important to control a wide range of reactions from gene expression to protein degradation in a cell-sized space. To bring a better understanding of the compatibility of such phase-separated…

Soft Condensed Matter · Physics 2021-10-01 Shuzo Kato , David Garenne , Vincent Noireaux , Yusuke T. Maeda

Liquid-liquid phase separation is now recognized as a common mechanism for regulating enzyme activity in cells. Insights from studies in cells are complemented by in vitro studies aimed at developing better understanding of mechanisms…

Biomolecules · Quantitative Biology 2020-10-23 Brian G. O'Flynn , Tanja Mittag

Proteins can form droplets via liquid-liquid phase separation (LLPS) in cells. Recent experiments demonstrate that LLPS is qualitatively different on two-dimensional (2d) surfaces compared to three-dimensional (3d) solutions. In this paper,…

Quantitative Methods · Quantitative Biology 2024-08-29 Jinyoung Kim , Sean D. Lawley , Jinsu Kim

Motility-induced phase separation (MIPS) arises generically in fluids of self-propelled particles when interactions lead to a kinetic slowdown at high densities. Starting from a continuum description of scalar active matter, akin to a…

Statistical Mechanics · Physics 2018-02-28 Alexandre P. Solon , Joakim Stenhammar , Michael E. Cates , Yariv Kafri , Julien Tailleur

The equilibrium silica liquid-liquid interface between the high-density liquid (HDL) phase and the low-density liquid (LDL) phase is examined using molecular-dynamics simulation. The structure, thermodynamics, and dynamics within the…

Materials Science · Physics 2022-10-19 Xin Zhang , Brian B. Laird , Hongtao Liang , Wenliang Lu , Zhiyong Yu , Xiangming Ma , Ya Cheng , Yang Yang

Liquid liquid phase separation (LLPS) mediated by pi-cation bonds between tyrosine and arginine residues are of biological importance. To understand the interactions between proteins in the condensed phase in close analogy to complex…

Biological Physics · Physics 2020-08-19 Aditya N. Singh , Arun Yethiraj

Biomolecular condensates undergirded by phase separations of proteins and nucleic acids serve crucial biological functions. To gain physical insights into their genetic basis, we study how liquid-liquid phase separation (LLPS) of…

Biomolecules · Quantitative Biology 2018-12-03 Suman Das , Alan Amin , Yi-Hsuan Lin , Hue Sun Chan

Linearly-sloped or `ramp' potentials belong to a class of core-softened models which possess a liquid-liquid critical point (LLCP) in addition to the usual liquid-gas critical point. Furthermore they exhibit thermodynamic anomalies in the…

Statistical Mechanics · Physics 2009-11-11 Helen M. Gibson , Nigel B. Wilding

A growing body of theoretical and experimental evidence strongly supports the existence of a second liquid-liquid critical point (LLCP) in deeply supercooled water leading to the co-existence of two phases: a high-and low-density liquid…

Soft Condensed Matter · Physics 2026-04-17 Cesare Malosso , Edward Danquah Donkor , Stefano Baroni , Ali Hassanali