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Adaptive optics (AO) is a powerful image correction technique with proven benefits for many life-science microscopy methods. However, the complexity of adding a reflective wavefront modulator and a wavefront sensor into already complicated…

Adaptive optics (AO) has revolutionized imaging in {fields} from astronomy to microscopy by correcting optical aberrations. In label-free microscopes, however, conventional AO faces limitations due to the absence of guidestar and the need…

The resolution and contrast of microscope imaging is often affected by aberrations introduced by imperfect optical systems and inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these aberrations and restores…

Adaptive optics (AO) are reconfigurable devices that compensate for wavefront distortions or aberrations in optical systems such as microscopes, telescopes and ophthalmoscopes. Aberrations have detrimental effects that can reduce imaging…

Optics · Physics 2025-06-10 Biwei Zhang , Martin J. Booth , Qi Hu

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Multifocal plane microscopy (MUM) allows three dimensional objects to be imaged in a single camera frame. Our approach uses dual orthogonal diffraction phase gratings with a quadratic distortion of the lines to apply defocus to the first…

Optics · Physics 2020-03-13 Benjamin Gore , Noah Schwartz , Paul Dalgarno

Adaptive optics (AO) is a powerful tool to increase the imaging depth of multiphoton scanning microscopes. For highly scattering tissues, sensorless wavefront correction techniques exhibit robust performance and present a straight-forward…

Astronomical telescopes suffer from a tradeoff between field of view (FoV) and image resolution: increasing the FoV leads to an optical field that is under-sampled by the science camera. This work presents a novel computational imaging…

Instrumentation and Methods for Astrophysics · Physics 2025-08-07 Robin Swanson , Esther Y. H. Lin , Masen Lamb , Suresh Sivanandam , Kiriakos N. Kutulakos

Adaptive optics (AO) is a powerful tool employed across various research fields, from aerospace to microscopy. Traditionally, AO has focused on correcting optical phase aberrations, with recent advances extending to polarisation…

Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…

Adaptive optics (AO) is critical in astronomy, optical communications and remote sensing to deal with the rapid blurring caused by the Earth's turbulent atmosphere. But current AO systems are limited by their wavefront sensors, which need…

Instrumentation and Methods for Astrophysics · Physics 2020-10-23 Barnaby R. M. Norris , Jin Wei , Christopher H. Betters , Alison Wong , Sergio G. Leon-Saval

Adaptive optics is a strategy to compensate for sample-induced aberrations in microscopy applications. Generally, it requires the presence of "guide stars" in the sample to serve as localized reference targets. We describe an implementation…

Three-photon (3-P) fluorescence microscopy enables deep in vivo imaging with subcellular resolution, but its performance is fundamentally constrained by the maximum permissible laser power required to avoid tissue heating and photodamage.…

Optics · Physics 2026-03-04 Qi Hu , Jingyu Wang , Huriye Atilgan , Armin Lak , Martin J. Booth

This work introduces the first closed-loop adaptive optics (AO) system capable of optically correcting aberrations in real-time without a guidestar or a wavefront sensor. Nearly 40 years ago, Cederquist et al. demonstrated that asymmetric…

Image and Video Processing · Electrical Eng. & Systems 2026-05-14 Weiyun Jiang , Haiyun Guo , Christopher A. Metzler , Ashok Veeraraghavan

Future large space telescopes will be equipped with adaptive optics (AO) to overcome wavefront aberrations and achieve high contrast for imaging faint astronomical objects, such as earth-like exoplanets and debris disks. In contrast to AO…

Instrumentation and Methods for Astrophysics · Physics 2020-01-07 He Sun , N. Jeremy Kasdin , Robert Vanderbei

High-contrast imaging systems using active control with adaptive optics (AO) are often limited by non-common path (NCP) aberrations that are seen only at the final science image. AO systems employing focal-plane wavefront sensors (FP-WFSs)…

Instrumentation and Methods for Astrophysics · Physics 2026-01-22 Aditya R. Sengupta , Lisa A. Poyneer , Benjamin L. Gerard , Rebecca Jensen-Clem , Aaron J. Lemmer

Adaptive optics (AO) systems and image reconstruction algorithms are indispensable tools when it comes to high-precision astrometry. In this paper, we analyze the potential of combining both techniques, i.e. by applying image reconstruction…

Instrumentation and Methods for Astrophysics · Physics 2019-03-13 Felix Bosco , Jörg-Uwe Pott , Rainer Schödel

A limitation of many compressive imaging architectures lies in the sequential nature of the sensing process, which leads to long sensing times. In this paper we present a novel architecture that uses fewer detectors than the number of…

Computer Vision and Pattern Recognition · Computer Science 2013-11-05 Tomas Björklund , Enrico Magli

Single-pixel imaging, originally developed in light optics, facilitates fast three-dimensional sample reconstruction, as well as probing with light wavelengths undetectable by conventional multi-pixel detectors. However, the spatial…

Inhomogeneities in the refractive index of a biological sample can introduce phase aberrationsin microscopy systems, severely impairing the quality of images. Adaptive optics can be employed to correct for phase aberrations and improve…

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