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Histological analysis of tissue samples is one of the most widely used methods for disease diagnosis. After taking a sample from a patient, it goes through a lengthy and laborious preparation, which stains the tissue to visualize different…

Computer Vision and Pattern Recognition · Computer Science 2019-03-05 Yair Rivenson , Hongda Wang , Zhensong Wei , Yibo Zhang , Harun Gunaydin , Aydogan Ozcan

We propose a virtual staining methodology based on Generative Adversarial Networks to map histopathology images of breast cancer tissue from H&E stain to PHH3 and vice versa. We use the resulting synthetic images to build Convolutional…

Image and Video Processing · Electrical Eng. & Systems 2020-03-18 Caner Mercan , Germonda Reijnen-Mooij , David Tellez Martin , Johannes Lotz , Nick Weiss , Marcel van Gerven , Francesco Ciompi

3D reconstruction is a challenging current topic in medical research. We perform 3D reconstructions from serial sections stained by immunohistological methods. This paper presents an immersive visualisation solution to quality control (QC),…

Graphics · Computer Science 2021-03-23 Oleg Lobachev , Moritz Berthold , Henriette Pfeffer , Michael Guthe , Birte S. Steiniger

Tissue biopsy evaluation in the clinic is in need of quantitative disease markers for diagnosis and, most importantly, prognosis. Among the new technologies, quantitative phase imaging (QPI) has demonstrated promise for histopathology…

The field of histology relies heavily on antiquated tissue processing and staining techniques that limit the efficiency of pathologic diagnoses of cancer and other diseases. Current staining and advanced labeling methods are often…

Histochemical staining is essential for visualizing tissue architecture and cellular morphology but is destructive and limited by the availability of tissue for multiple stains. Virtual staining with label-free microscopy offers a…

Histopathology images; microscopy images of stained tissue biopsies contain fundamental prognostic information that forms the foundation of pathological analysis and diagnostic medicine. However, diagnostics from histopathology images…

Computer Vision and Pattern Recognition · Computer Science 2019-10-30 Aïcha BenTaieb , Ghassan Hamarneh

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

Pathological diagnosis relies on the visual inspection of histologically stained thin tissue specimens, where different types of stains are applied to bring contrast to and highlight various desired histological features. However, the…

Medical Physics · Physics 2022-08-18 Xilin Yang , Bijie Bai , Yijie Zhang , Yuzhu Li , Kevin de Haan , Tairan Liu , Aydogan Ozcan

Histological examination is a crucial step in an autopsy; however, the traditional histochemical staining of post-mortem samples faces multiple challenges, including the inferior staining quality due to autolysis caused by delayed fixation…

Digital whole-slide images of pathological tissue samples have recently become feasible for use within routine diagnostic practice. These gigapixel sized images enable pathologists to perform reviews using computer workstations instead of…

Human-Computer Interaction · Computer Science 2016-10-14 Jesper Molin , Anna Bodén , Darren Treanor , Morten Fjeld , Claes Lundström

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…

Instrumentation and Detectors · Physics 2023-12-21 Michela Esposito , Nicole Schieber , Alessandro Olivo , Yannick Schwab , Marco Endrizzi

Microscopy enables direct observation of cellular morphology in 3D, with transmitted-light methods offering low-cost, minimally invasive imaging and fluorescence microscopy providing specificity and contrast. Virtual staining combines these…

Virtual staining leverages computer-aided techniques to transfer the style of histochemically stained tissue samples to other staining types. In virtual staining of pathological images, maintaining strict structural consistency is crucial,…

Image and Video Processing · Electrical Eng. & Systems 2024-12-17 Bing Xiong , Yue Peng , RanRan Zhang , Fuqiang Chen , JiaYe He , Wenjian Qin

Histopathological staining of human tissue is essential for disease diagnosis. Recent advances in virtual tissue staining technologies using artificial intelligence (AI) alleviate some of the costly and tedious steps involved in traditional…

Image and Video Processing · Electrical Eng. & Systems 2025-06-17 Luzhe Huang , Yuzhu Li , Nir Pillar , Tal Keidar Haran , William Dean Wallace , Aydogan Ozcan

Virtual staining offers a promising method for converting Hematoxylin and Eosin (H&E) images into Immunohistochemical (IHC) images, eliminating the need for costly chemical processes. However, existing methods often struggle to utilize…

Computer Vision and Pattern Recognition · Computer Science 2025-11-11 Sicheng Yang , Zhaohu Xing , Haipeng Zhou , Lei Zhu

The diagnosis of cancer is mainly performed by visual analysis of the pathologists, through examining the morphology of the tissue slices and the spatial arrangement of the cells. If the microscopic image of a specimen is not stained, it…

Image and Video Processing · Electrical Eng. & Systems 2020-02-06 Pegah Salehi , Abdolah Chalechale

Imaging with hard x-rays is an invaluable tool in medicine, biology, materials science, and cultural heritage. Propagation-based x-ray phase-contrast imaging and tomography have been mostly used to resolve micrometer-scale structures inside…

Image and Video Processing · Electrical Eng. & Systems 2025-06-02 Margie P. Olbinado , David M. Paganin , Yin Cheng , Alexander Rack

Multiple instance learning (MIL) is the dominant framework for whole-slide image analysis in computational pathology, typically combining a frozen patch encoder, a projection layer, and a slide-level aggregator. While encoders and…

Quantitative Methods · Quantitative Biology 2026-05-19 Yucheng Xing , Pei Liu , Jingying Ma , Ruping Hong , Jiangdong Qiu , Tianyu Liu , Kai He , Ling Huang , Mengling Feng

Hematoxylin and eosin (H&E) staining visualizes histology but lacks specificity for diagnostic markers. Immunohistochemistry (IHC) staining provides protein-targeted staining but is restricted by tissue availability and antibody…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Anran Liu , Xiaofei Wang , Jing Cai , Chao Li