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Related papers: FAIR sharing of Chromatin Tracing datasets using t…

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Karyotyping is a process in which chromosomes in a dividing cell are properly stained, identified and displayed in a standard format, which helps geneticist to study and diagnose genetic factors behind various genetic diseases and for…

Computer Vision and Pattern Recognition · Computer Science 2012-05-10 K. S. Sreejini , A. Lijiya , V. K. Govindan

3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…

Image and Video Processing · Electrical Eng. & Systems 2019-11-28 Yang Jiao , Mo Weng , Mei Yang

Many biological and soft matter processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding experimental challenge. Here, we introduce Fourier…

Simultaneous identification and metabolic analysis of microbes with single-cell resolution and high throughput is necessary to answer the question of "who eats what, when, and where" in complex microbial communities. Here, we present a…

Biological Physics · Physics 2022-09-13 Yeran Bai , Zhongyue Guo , Fátima C. Pereira , Michael Wagner , Ji-Xin Cheng

Optical Coherence Tomography (OCT) is a novel and effective screening tool for ophthalmic examination. Since collecting OCT images is relatively more expensive than fundus photographs, existing methods use multi-modal learning to complement…

Image and Video Processing · Electrical Eng. & Systems 2023-08-02 Lehan Wang , Weihang Dai , Mei Jin , Chubin Ou , Xiaomeng Li

While the FAIR principles are well accepted in the scientific community, the implementation of appropriate metadata editing and transfer to ensure FAIR research data in practice is significantly lagging behind. On the one hand, it strongly…

We present a novel method for real-time monitoring and kinetic analysis of fluorescence in situ hybridization (FISH). We implement the method using a vertical microfluidic probe containing a microstructure designed for rapid switching…

Biomolecules · Quantitative Biology 2018-10-04 Nadya Ostromohov , Deborah Huber , Moran Bercovici , Govind V. Kaigala

We have developed a three-dimensional radiative transfer method designed specifically for use with parallel adaptive mesh refinement hydrodynamics codes. This new algorithm, which we call hybrid characteristics, introduces a novel form of…

Astrophysics · Physics 2009-11-11 Erik-Jan Rijkhorst , Tomasz Plewa , Anshu Dubey , Garrelt Mellema

Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as a label-free imaging tool, capable of resolving cell types and organelles within 3D live samples, whilst monitoring their activity at tens of milliseconds…

Multicolor in situ hybridization (mFISH) is a karyotyping technique used to detect major chromosomal alterations using fluorescent probes and imaging techniques. Manual interpretation of mFISH images is a time consuming step that can be…

Computer Vision and Pattern Recognition · Computer Science 2018-05-04 Esteban Pardo , José Mário T Morgado , Norberto Malpica

High-throughput and quantitative experimental technologies are experiencing rapid advances in the biological sciences. One important recent technique is multiplexed fluorescence in situ hybridization (mFISH), which enables the…

Information Theory · Computer Science 2021-02-04 Luca D'Alessio , Litian Liu , Ken Duffy , Yonina C. Eldar , Muriel Medard , Mehrtash Babadi

In this study, we propose a novel federated learning (FL) approach that utilizes 3D style transfer for the multi-organ segmentation task. The multi-organ dataset, obtained by integrating multiple datasets, has high scalability and can…

Computer Vision and Pattern Recognition · Computer Science 2024-10-29 Yuto Shibata , Yasunori Kudo , Yohei Sugawara

Involved in mitotic condensation, interaction of transcriptional regulatory elements or isolation of structural domains, understanding loop formation is becoming a paradigm in the deciphering of chromatin architecture and its functional…

Quantitative Methods · Quantitative Biology 2024-09-17 Michaël Liefsoens , Timothy Földes , Maria Barbi

Selective plane illumination microscopy (SPIM), also known as light sheet fluorescence microscopy, provides high specificity through fluorescence labeling. However, it lacks complementary structural information from the surrounding context,…

Optics · Physics 2024-12-19 Rui Ma , Olga Lyraki , Daniel Wehner , Jochen Guck

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method…

Optics · Physics 2025-09-11 Charlie Kersuzan , Anirban Jana , Amaury Badon

This paper describes a micro fluorescence in situ hybridization ({\mu}FISH)-based rapid detection of cytogenetic biomarkers on formalin-fixed paraffin embedded (FFPE) tissue sections. We demonstrated this method in the context of detecting…

Tissues and Organs · Quantitative Biology 2018-06-19 Deborah Huber , Govind V. Kaigala

Labelling data is expensive and time consuming especially for domains such as medical imaging that contain volumetric imaging data and require expert knowledge. Exploiting a larger pool of labeled data available across multiple centers,…

Computer Vision and Pattern Recognition · Computer Science 2020-09-03 Daiqing Li , Amlan Kar , Nishant Ravikumar , Alejandro F Frangi , Sanja Fidler

Deep learning-based organs/structures-at-risk(OARs) auto-contouring models can improve radiotherapy workflows, but models trained on adult data often underperform in pediatric patients. Developing robust pediatric-specific models is…

Three-dimensional (3D) chromatin structure is closely related to genome function, in particular transcription. However, the folding path of the chromatin fiber in the interphase nucleus is unknown. Here, we systematically measured the 3D…

High-fidelity patient-specific modeling of cardiovascular flows and hemodynamics is challenging. Direct blood flow measurement inside the body with in-vivo measurement modalities such as 4D flow magnetic resonance imaging (4D flow MRI)…

Fluid Dynamics · Physics 2021-06-11 Milad Habibi , Roshan M. D'Souza , Scott T. M. Dawson , Amirhossein Arzani
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