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Recent advancements in miniaturized fluorescence microscopy have made it possible to investigate neuronal responses to external stimuli in awake behaving animals through the analysis of intra-cellular calcium signals. An on-going challenge…

Applications · Statistics 2022-01-28 Laura D'Angelo , Antonio Canale , Zhaoxia Yu , Michele Guindani

Fluorescence Microcopy Calcium Imaging is a fundamental tool to in-vivo record and analyze large scale neuronal activities simultaneously at a single cell resolution. Automatic and precise detection of behaviorally relevant neuron activity…

Computer Vision and Pattern Recognition · Computer Science 2025-12-23 Ran Li , Pan Xiao , Kaushik Dutta , Youdong Guo

Advances in neuroscience have enabled researchers to measure the activities of large numbers of neurons simultaneously in behaving animals. We have access to the fluorescence of each of the neurons which provides a first-order approximation…

Neurons and Cognition · Quantitative Biology 2023-07-21 Abhisek Chakraborty

Calcium imaging allows for the parallel measurement of large neuronal populations in a spatially resolved and minimally invasive manner, and has become a gold-standard for neuronal functionality. While deep generative models have been…

Neurons and Cognition · Quantitative Biology 2025-10-02 Berta Ros , Mireia Olives-Verger , Caterina Fuses , Josep M Canals , Jordi Soriano , Jordi Abante

We present efficient Bayesian methods for extracting neuronal spiking information from calcium imaging data. The goal of our methods is to sample from the posterior distribution of spike trains and model parameters (baseline concentration,…

Neurons and Cognition · Quantitative Biology 2013-11-28 Eftychios A. Pnevmatikakis , Josh Merel , Ari Pakman , Liam Paninski

Deducing the structure of neural circuits is one of the central problems of modern neuroscience. Recently-introduced calcium fluorescent imaging methods permit experimentalists to observe network activity in large populations of neurons,…

Applications · Statistics 2011-07-22 Yuriy Mishchencko , Joshua T. Vogelstein , Liam Paninski

Calcium imaging permits optical measurement of neural activity. Since intracellular calcium concentration is an indirect measurement of neural activity, computational tools are necessary to infer the true underlying spiking activity from…

Machine Learning · Statistics 2017-11-07 Artur Speiser , Jinyao Yan , Evan Archer , Lars Buesing , Srinivas C. Turaga , Jakob H. Macke

We present a structured matrix factorization approach to analyzing calcium imaging recordings of large neuronal ensembles. Our goal is to simultaneously identify the locations of the neurons, demix spatially overlapping components, and…

Neurons and Cognition · Quantitative Biology 2014-09-11 Eftychios A. Pnevmatikakis , Yuanjun Gao , Daniel Soudry , David Pfau , Clay Lacefield , Kira Poskanzer , Randy Bruno , Rafael Yuste , Liam Paninski

Calcium imaging data promises to transform the field of neuroscience by making it possible to record from large populations of neurons simultaneously. However, determining the exact moment in time at which a neuron spikes, from a calcium…

Methodology · Statistics 2018-02-22 Sean Jewell , Toby Dylan Hocking , Paul Fearnhead , Daniela Witten

We study noisy calcium imaging data, with a focus on the classification of spike traces. As raw traces obscure the true temporal structure of neuron's activity, we performed a tuned filtering of the calcium concentration using two methods:…

Neurons and Cognition · Quantitative Biology 2024-09-17 Arianna Burzacchi , Nicoletta D'Angelo , David Payares-Garcia , Jorge Mateu

Quantification of neuronal correlations in neuron populations helps us to understand neural coding rules. Such quantification could also reveal how neurons encode information in normal and disease conditions like Alzheimer's and…

Neurons and Cognition · Quantitative Biology 2021-05-10 Sathish Ande , Srinivas Avasarala , Ajith Karunarathne , Lopamudra Giri , Soumya Jana

Calcium imaging is an important technique for monitoring the activity of thousands of neurons simultaneously. As calcium imaging datasets grow in size, automated detection of individual neurons is becoming important. Here we apply a…

Neurons and Cognition · Quantitative Biology 2016-12-23 Noah J. Apthorpe , Alexander J. Riordan , Rob E. Aguilar , Jan Homann , Yi Gu , David W. Tank , H. Sebastian Seung

Optical imaging of genetically encoded calcium indicators is a powerful tool to record the activity of a large number of neurons simultaneously over a long period of time from freely behaving animals. However, determining the exact time at…

Applications · Statistics 2021-03-08 Tong Shen , Kevin Johnston , Gyorgy Lur , Michele Guindani , Hernando Ombao , Zhaoxia Yu

Calcium imaging is one of the most important tools in neurophysiology as it enables the observation of neuronal activity for hundreds of cells in parallel and at single-cell resolution. In order to use the data gained with calcium imaging,…

Neurons and Cognition · Quantitative Biology 2020-04-07 Elke Kirschbaum , Alberto Bailoni , Fred A. Hamprecht

In the past few years, new technologies in the field of neuroscience have made it possible to simultaneously image activity in large populations of neurons at cellular resolution in behaving animals. In mid-2016, a huge repository of this…

Applications · Statistics 2017-03-22 Ashley Petersen , Noah Simon , Daniela Witten

Calcium imaging has become a fundamental neural imaging technique, aiming to recover the individual activity of hundreds of neurons in a cortical region. Current methods (mostly matrix factorization) are aimed at detecting neurons in the…

Applications · Statistics 2019-02-11 Gal Mishne , Adam S. Charles

Neurons encode information about the environment through their activity. As animals explore the environment, neurons rapidly acquire selectivity for distinct features of the external world; characterizing how these selectivity patterns…

We propose a scalable semiparametric Bayesian model to capture dependencies among multiple neurons by detecting their co-firing (possibly with some lag time) patterns over time. After discretizing time so there is at most one spike at each…

Applications · Statistics 2015-06-22 Babak Shahbaba , Bo Zhou , Shiwei Lan , Hernando Ombao , David Moorman , Sam Behseta

With the increasing popularity of calcium imaging data in neuroscience research, methods for analyzing calcium trace data are critical to address various questions. The observed calcium traces are either analyzed directly or deconvolved to…

Neurons and Cognition · Quantitative Biology 2021-03-04 Tong Shen , Gyorgy Lur , Xiangmin Xu , Zhaoxia Yu

A fundamental challenge in calcium imaging has been to infer the timing of action potentials from the measured noisy calcium fluorescence traces. We systematically evaluate a range of spike inference algorithms on a large benchmark dataset…

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