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Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

X-ray free-electron lasers (XFELs) offer unique capabilities for measuring the structure and dynamics of biomolecules, helping us understand the basic building blocks of life. Notably, high-repetition-rate XFELs enable single particle…

Computer Vision and Pattern Recognition · Computer Science 2023-12-25 Jay Shenoy , Axel Levy , Frédéric Poitevin , Gordon Wetzstein

The development of X-ray Free Electron Lasers (XFELs) has opened numerous opportunities to probe atomic structure and ultrafast dynamics of various materials. Single Particle Imaging (SPI) with XFELs enables the investigation of biological…

Computer Vision and Pattern Recognition · Computer Science 2023-11-29 Zhantao Chen , Cong Wang , Mingye Gao , Chun Hong Yoon , Jana B. Thayer , Joshua J. Turner

X-ray single particle imaging involves the measurement of a large number of noisy diffraction patterns of isolated objects in random orientations. The missing information about these patterns is then computationally recovered in order to…

Image and Video Processing · Electrical Eng. & Systems 2020-05-25 Kartik Ayyer

Single-particle imaging (SPI) using X-ray free-electron Lasers (XFELs) offers the potential to determine protein structures at high spatial and temporal resolutions without the need for crystallization or vitrification. However, the…

Biomolecules · Quantitative Biology 2024-11-26 Tong You , Johan Bielecki , Filipe R. N. C. Maia

A well-characterised wavefront is important for many X-ray free-electron laser (XFEL) experiments, especially for single-particle imaging (SPI), where individual bio-molecules randomly sample a nanometer-region of highly-focused femtosecond…

Single-pixel imaging (SPI) is an emerging technique which has attracts wide attention in various research fields. However, restricted by the low reconstruction quality and large amount of measurements, the practical application is still in…

Image and Video Processing · Electrical Eng. & Systems 2018-11-09 Chao Deng , Xuemei Hu , Xiaoxu Li , Jinli Suo , Zhili Zhang , Qionghai Dai

Imaging of the structure of single proteins or other biomolecules with atomic resolution would be enormously beneficial to structural biology. X-ray free-electron lasers generate highly intense and ultrashort x-ray pulses, providing a route…

Atomic Physics · Physics 2014-07-30 Jan Malte Slowik , Sang-Kil Son , Gopal Dixit , Zoltan Jurek , Robin Santra

An improved analysis for single particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the AMO instrument at the Linac Coherent Light Source (LCLS) as…

Imaging the structure and observing the dynamics of isolated proteins using single-particle X-ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers (XFELs). Currently, SPI experiments on isolated…

We propose an encryption-decryption framework for validating diffraction intensity volumes reconstructed using single-particle imaging (SPI) with x-ray free-electron lasers (XFELs) when the ground truth volume is absent. This framework…

Data Analysis, Statistics and Probability · Physics 2020-08-03 Zhou Shen , Colin Zhi Wei Teo , Kartik Ayyer , N. Duane Loh

The evolution of X-ray detection technology has significantly enhanced sensitivity and spatial resolution in non-destructive imaging of internal structure. However, the problem of low luminescence and transparency of scintillator materials…

Optics · Physics 2025-07-16 Tatsunori Shibuya , Eichi Terasawa , Hiromi Kimura , Takeshi Fujiwara

Obtaining 3D information from a single X-ray exposure at high-brilliance sources, such as X-ray free-electron lasers (XFELs) [1] or diffraction-limited storage rings [2], allows the study of fast dynamical processes in their native…

Single-Pixel Imaging (SPI) enables the reconstruction of objects using a single detector through sequential illuminations with structured light patterns. The choice of illumination patterns is critical, particularly in highly undersampled…

Computer Vision and Pattern Recognition · Computer Science 2026-04-28 Serban Cristian Tudosie , Alexander Denker , Zeljko Kereta , Simon Arridge

The Linac Coherent Light Source (LCLS) is an X- ray free electron laser (XFEL) facility enabling the study of the structure and dynamics of single macromolecules. A major upgrade will bring the repetition rate of the X-ray source from 120…

Computational Physics · Physics 2021-09-14 Hsing-Yin Chang , Elliott Slaughter , Seema Mirchandaney , Jeffrey Donatelli , Chun Hong Yoon

This work proposes an innovative approach to improve Bragg coherent diffraction imaging (BCDI) microscopy applied to time evolving crystals and/or non-homogeneous crystalline strain fields, identified as two major limitations of BCDI…

Optics · Physics 2024-03-12 I. Calvo-Almazán , V. Chamard , T. Grünewald , M. Allain

The advent of X-ray Free Electron Lasers promises the possibility to determine the structure of individual particles such as microcrystallites, viruses and biomolecules from single-shot diffraction snapshots obtained before the particle is…

Soft Condensed Matter · Physics 2010-05-06 Brian Moths , Abbas Ourmazd

Single pixel imaging (SPI) is a novel technique being able to capture 2D images using a bucket detector with high signal-to-noise ratio, wide spectrum range and low cost. Conventional SPI projects random illumination patterns to randomly…

Optics · Physics 2016-07-19 Liheng Bian , Jinli Suo , Xuemei Hu , Feng Chen , Qionghai Dai
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