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Virtual staining is a promising technique that uses deep generative models to recreate histological stains, providing a faster and more cost-effective alternative to traditional tissue chemical staining. Specifically for H&E-HER2 staining…

Image and Video Processing · Electrical Eng. & Systems 2025-10-02 Pascal Klöckner , José Teixeira , Diana Montezuma , Jaime S. Cardoso , Hugo M. Horlings , Sara P. Oliveira

Quantitative analysis of cell structures is essential for biomedical and pharmaceutical research. The standard imaging approach relies on fluorescence microscopy, where cell structures of interest are labeled by chemical staining…

Accurate and fast histological staining is crucial in histopathology, impacting diagnostic precision and reliability. Traditional staining methods are time-consuming and subjective, causing delays in diagnosis. Digital pathology plays a…

Image and Video Processing · Electrical Eng. & Systems 2023-07-06 Marian Boktor , James E. D. Tweel , Benjamin R. Ecclestone , Jennifer Ai Ye , Paul Fieguth , Parsin Haji Reza

Deep learning models can generate virtual immunohistochemistry (IHC) stains from hematoxylin and eosin (H&E) images, offering a scalable and low-cost alternative to laboratory IHC. However, reliable evaluation of image quality remains a…

Computer Vision and Pattern Recognition · Computer Science 2025-11-07 Tushar Kataria , Shikha Dubey , Mary Bronner , Jolanta Jedrzkiewicz , Ben J. Brintz , Shireen Y. Elhabian , Beatrice S. Knudsen

We present fast, realistic image generation on high-resolution, multimodal datasets using hierarchical variational autoencoders (VAEs) trained on a deterministic autoencoder's latent space. In this two-stage setup, the autoencoder…

Computer Vision and Pattern Recognition · Computer Science 2023-03-27 Troy Luhman , Eric Luhman

H&E-to-IHC stain translation techniques offer a promising solution for precise cancer diagnosis, especially in low-resource regions where there is a shortage of health professionals and limited access to expensive equipment. Considering the…

Image and Video Processing · Electrical Eng. & Systems 2024-09-04 Song Wang , Zhong Zhang , Huan Yan , Ming Xu , Guanghui Wang

In the field of deep learning, large architectures often obtain the best performance for many tasks, but also require massive datasets. In the histological domain, tissue images are expensive to obtain and constitute sensitive medical…

Computer Vision and Pattern Recognition · Computer Science 2025-04-08 Andrei-Alexandru Preda , Iulian-Marius Tăiatu , Dumitru-Clementin Cercel

Pathological diagnosis relies on the visual inspection of histologically stained thin tissue specimens, where different types of stains are applied to bring contrast to and highlight various desired histological features. However, the…

Medical Physics · Physics 2022-08-18 Xilin Yang , Bijie Bai , Yijie Zhang , Yuzhu Li , Kevin de Haan , Tairan Liu , Aydogan Ozcan

The emergence of virtual staining technology provides a rapid and efficient alternative for researchers in tissue pathology. It enables the utilization of unlabeled microscopic samples to generate virtual replicas of chemically stained…

Image and Video Processing · Electrical Eng. & Systems 2025-01-08 Junjia Wang , Bo Xiong , You Zhou , Xun Cao , Zhan Ma

Generative models have been applied in the medical imaging domain for various image recognition and synthesis tasks. However, a more controllable and interpretable image synthesis model is still lacking yet necessary for important…

Image and Video Processing · Electrical Eng. & Systems 2021-11-15 Jiarong Ye , Yuan Xue , Peter Liu , Richard Zaino , Keith Cheng , Xiaolei Huang

Training robust supervised deep learning models for many geospatial applications of computer vision is difficult due to dearth of class-balanced and diverse training data. Conversely, obtaining enough training data for many applications is…

Computer Vision and Pattern Recognition · Computer Science 2020-12-09 Xuerong Xiao , Swetava Ganguli , Vipul Pandey

Multiplex immunofluorescence (mIF) enables simultaneous single-cell quantification of multiple biomarkers within intact tissue architecture, yet its high reagent cost, multi-round staining protocols, and need for specialized imaging…

Computer Vision and Pattern Recognition · Computer Science 2026-03-18 Junhyeok Lee , Han Jang , Heeseong Eum , Joon Jang , Kyu Sung Choi

Generative AI has received substantial attention in recent years due to its ability to synthesize data that closely resembles the original data source. While Generative Adversarial Networks (GANs) have provided innovative approaches for…

Computer Vision and Pattern Recognition · Computer Science 2023-04-04 Xuan Xu , Saarthak Kapse , Rajarsi Gupta , Prateek Prasanna

The popular use of histopathology images, such as hematoxylin and eosin (H&E), has proven to be useful in detecting tumors. However, moving such cancer cases forward for treatment requires accurate on the amount of the human epidermal…

Computer Vision and Pattern Recognition · Computer Science 2025-12-30 Olaide N. Oyelade , Oliver Hoxey , Yulia Humrye

Virtual immunohistochemistry (IHC) aims to computationally synthesize molecular staining patterns from routine Hematoxylin and Eosin (H\&E) images, offering a cost-effective and tissue-efficient alternative to traditional physical staining.…

Computer Vision and Pattern Recognition · Computer Science 2026-01-23 Rongze Ma , Mengkang Lu , Zhenyu Xiang , Yongsheng Pan , Yicheng Wu , Qingjie Zeng , Yong Xia

Breast cancer presents a significant healthcare challenge globally, demanding precise diagnostics and effective treatment strategies, where histopathological examination of Hematoxylin and Eosin (H&E) stained tissue sections plays a central…

Image and Video Processing · Electrical Eng. & Systems 2024-08-06 Linhao Qu , Chengsheng Zhang , Guihui Li , Haiyong Zheng , Chen Peng , Wei He

Due to its superior efficiency in utilizing annotations and addressing gigapixel-sized images, multiple instance learning (MIL) has shown great promise as a framework for whole slide image (WSI) classification in digital pathology…

Quantitative Methods · Quantitative Biology 2023-07-14 Qiehe Sun , Jiawen Li , Jin Xu , Junru Cheng , Tian Guan , Yonghong He

In whole slide imaging, commonly used staining techniques based on hematoxylin and eosin (H&E) and immunohistochemistry (IHC) stains accentuate different aspects of the tissue landscape. In the case of detecting metastases, IHC provides a…

Image and Video Processing · Electrical Eng. & Systems 2022-08-30 Joseph Boyd , Irène Villa , Marie-Christine Mathieu , Eric Deutsch , Nikos Paragios , Maria Vakalopoulou , Stergios Christodoulidis

Hematoxylin and eosin (H&E) staining is the clinical standard for assessing tissue morphology, but it lacks molecular-level diagnostic information. In contrast, immunohistochemistry (IHC) provides crucial insights into biomarker expression,…

Image and Video Processing · Electrical Eng. & Systems 2025-08-05 Jingsong Liu , Xiaofeng Deng , Han Li , Azar Kazemi , Christian Grashei , Gesa Wilkens , Xin You , Tanja Groll , Nassir Navab , Carolin Mogler , Peter J. Schüffler

For invasive breast cancer, immunohistochemical (IHC) techniques are often used to detect the expression level of human epidermal growth factor receptor-2 (HER2) in breast tissue to formulate a precise treatment plan. From the perspective…