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Photoacoustic microscopy holds the potential to measure biomarkers' structural and functional status without labels, which significantly aids in comprehending pathophysiological conditions in biomedical research. However, conventional…

Image and Video Processing · Electrical Eng. & Systems 2025-06-05 Wangting Zhou , Jiangshan He , Tong Cai , Lin Wang , Zhen Yuan , Xunbin Wei , Xueli Chen

Many biological and soft matter processes occur at high speeds in complex 3D environments, and developing imaging techniques capable of elucidating their dynamics is an outstanding experimental challenge. Here, we introduce Fourier…

Optical coherence tomography (OCT) is a powerful and noninvasive method for retinal imaging. In this paper, we introduce a fast segmentation method based on a new variant of spectral graph theory named diffusion maps. The research is…

Computer Vision and Pattern Recognition · Computer Science 2012-10-09 Raheleh Kafieh , Hossein Rabbani , Michael D. Abramoff , Milan Sonka

Fiber-based micro-endoscopes are a critically important tool for minimally-invasive deep-tissue imaging. However, the state-of-the-art micro-endoscopes cannot perform three-dimensional imaging through dynamically-bent fibers without the use…

Optics · Physics 2022-11-09 Noam Badt , Ori Katz

Serial sectioning Optical Coherence Tomography (sOCT) is a high-throughput, label free microscopic imaging technique that is becoming increasingly popular to study post-mortem neurovasculature. Quantitative analysis of the vasculature…

Image and Video Processing · Electrical Eng. & Systems 2024-05-24 Etienne Chollet , Yael Balbastre , Caroline Magnain , Bruce Fischl , Hui Wang

We propose a new strategy for obtaining enhanced resolution (FWHM = 0.12 arcsec) deep optical images over a wide field of view. As is well known, this type of image quality can be obtained in principle simply by fast guiding on a small (D =…

Astrophysics · Physics 2009-10-31 N. Kaiser , J. L. Tonry , G. A. Luppino

Background: Cervical cancer seriously affects the health of the female reproductive system. Optical coherence tomography (OCT) emerged as a non-invasive, high-resolution imaging technology for cervical disease detection. However, OCT image…

Image and Video Processing · Electrical Eng. & Systems 2022-06-14 Kaiyi Chen , Qingbin Wang , Yutao Ma

Optical Coherence Tomography (OCT) is essential for diagnosing conditions such as glaucoma, diabetic retinopathy, and age-related macular degeneration. Accurate retinal layer segmentation enables quantitative biomarkers critical for…

Image and Video Processing · Electrical Eng. & Systems 2025-09-10 S M Asiful Islam Saky , Ugyen Tshering

Ultrasound vascular imaging is limited by acoustic diffraction, restricting visualization of microvessels essential for understanding organ function and disease. Label-free super-resolution methods exploiting endogenous red blood cells have…

Clinical tracking systems are popular but typically require specific tracking markers. During the last years, scanning speed of optical coherence tomography (OCT) has increased to A-scan rates above 1 MHz allowing to acquire volume scans of…

Medical Physics · Physics 2019-01-15 Matthias Schlüter , Christoph Otte , Thore Saathoff , Nils Gessert , Alexander Schlaefer

Fluorescence microscopic imaging in centimeter-deep tissue has been highly sought-after for many years because much interesting in vivo micro-information, such as microcirculation, tumor angiogenesis, and metastasis, may deeply locate in…

We demonstrate a three-dimensional (3D) optical diffraction tomographic technique with optimal frequency combination (OFC-ODT) for the 3D quantitative phase imaging of unlabeled specimens. Three sets of through-focus intensity images are…

Optics · Physics 2018-03-06 Jiaji Li , Qian Chen Jiasong Sun , Jialin Zhang , Junyi Ding , Chao Zuo

Optical Coherence Tomography (OCT) is the primary imaging modality for detecting pathological biomarkers associated to retinal diseases such as Age-Related Macular Degeneration. In practice, clinical diagnosis and treatment strategies are…

Computer Vision and Pattern Recognition · Computer Science 2019-07-17 Thomas Kurmann , Pablo Márquez-Neila , Siqing Yu , Marion Munk , Sebastian Wolf , Raphael Sznitman

Dark-field X-ray microscopy (DFXM) is a novel X-ray imaging technique developed at synchrotrons to image along the diffracted beam with a real space resolution of ~100 nm and reciprocal space resolution of $10^{-4}$. Recent implementations…

Materials Science · Physics 2024-10-11 Darshan Chalise , Yifan Wang , Mariano Trigo , Leora E. Dresselhaus-Marais

Wide-field imaging is widely adopted due to its fast acquisition, cost-effectiveness and ease of use. Its extension to direct volumetric applications, however, is burdened by the trade-off between resolution and depth of field (DOF),…

Optical coherence tomography (OCT) is a non-invasive imaging modality which is widely used in clinical ophthalmology. OCT images are capable of visualizing deep retinal layers which is crucial for early diagnosis of retinal diseases. In…

Computer Vision and Pattern Recognition · Computer Science 2019-05-28 Peyman Gholami , Priyanka Roy , Mohana Kuppuswamy Parthasarathy , Vasudevan Lakshminarayanan

Optical diffraction tomography (ODT) has emerged as an important label-free tool in biomedicine to measure the three-dimensional (3D) structure of a biological sample. In this paper, we describe ODT using second-harmonic generation (SHG)…

Optics · Physics 2024-05-21 Amirhossein Saba , Carlo Gigli , Ye Pu , Demetri Psaltis

Retinal optical coherence tomography (OCT) images provide crucial insights into the health of the posterior ocular segment. Therefore, the advancement of automated image analysis methods is imperative to equip clinicians and researchers…

Image and Video Processing · Electrical Eng. & Systems 2024-02-16 Jiahao Wang , Hong Peng , Shengchao Chen , Sufen Ren

Linear phase-contrast scanning transmission electron microscopy (STEM) techniques compatible with high-throughput 4D-STEM acquisition are widely used to enhance phase contrast in weakly scattering and beam-sensitive materials. In these…

We introduce HAMscope, a compact, snapshot hyperspectral autofluorescence miniscope that enables real-time, label-free molecular imaging in a wide range of biological systems. By integrating a thin polymer diffuser into a widefield…