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Bacterial heterogeneity is pivotal for adaptation to diverse environments, posing significant challenges in microbial diagnostics and therapeutic interventions. Recent advancements in high-resolution optical microscopy have revolutionized…

Biological Physics · Physics 2025-01-06 Dohyeon Lee , Hyun-Seung Lee , Moosung Lee , Minhee Kang , Geon Kim , Tae Yeul Kim , Nam Yong Lee , YongKeun Park

Raster-scan optoacoustic mesoscopy (RSOM) is a powerful, non-invasive optical imaging technique for functional, anatomical, and molecular skin and tissue analysis. However, both the manual and the automated analysis of such images are…

Image and Video Processing · Electrical Eng. & Systems 2020-12-04 Stefan Gerl , Johannes C. Paetzold , Hailong He , Ivan Ezhov , Suprosanna Shit , Florian Kofler , Amirhossein Bayat , Giles Tetteh , Vasilis Ntziachristos , Bjoern Menze

We present a new digital holographic microscope (DHM) with a low coherent source for the quantitative imaging of smooth and optically rough objects. The experimental design of the microscope uses an in-line experimental geometry based on…

Using spectrally correlated photon pairs instead of classical laser light and coincidence detection instead of light intensity detection, Quantum Optical Coherence Tomography (Q-OCT) outperforms classical OCT in several experimental terms.…

Quantum Physics · Physics 2025-10-06 Sylwia M. Kolenderska , Crislane Vieira de Brito , Piotr Kolenderski

We demonstrate a new computational illumination technique that achieves large space-bandwidth-time product, for quantitative phase imaging of unstained live samples in vitro. Microscope lenses can have either large field of view (FOV) or…

Optics · Physics 2015-10-16 Lei Tian , Ziji Liu , Li-Hao Yeh , Michael Chen , Jingshan Zhong , Laura Waller

Currently, diagnosis of skin diseases is based primarily on visual pattern recognition skills and expertise of the physician observing the lesion. Even though dermatologists are trained to recognize patterns of morphology, it is still a…

Until recently, conventional biochemical staining had the undisputed status as well-established benchmark for most biomedical problems related to clinical diagnostics, fundamental research and biotechnology. Despite this role as…

The Segment Anything Model (SAM) has gained popularity as a versatile image segmentation method, thanks to its strong generalization capabilities across various domains. However, when applied to optic disc (OD) and optic cup (OC)…

Computer Vision and Pattern Recognition · Computer Science 2025-02-11 Jinchen Yu , Yongwei Nie , Fei Qi , Wenxiong Liao , Hongmin Cai

Conventionally, 3-dimensional cellular tomography can be done with light sheet or multi-angle observations. Recently, a new technique was introduced where the cell was rotated using convection currents to visualize the outer periphery (Liu…

Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties,…

Acoustic-resolution optoacoustic microscopy (AR-OAM) retrieves anatomical and functional contrast from living tissues at depths not reachable with optical microscopy. The imaging performance of AR-OAM has been advanced with image…

The orbital angular momentum (OAM) of light provides an unbounded set of orthogonal modes for ultrahigh-capacity optical information processing. However, current OAM detection schemes typically rely on light interference or diffraction,…

Multiple scattering of waves in disordered media is a nightmare whether it be for detection or imaging purposes. The best approach so far to get rid of multiple scattering is optical coherence tomography. It basically combines confocal…

Endoscopy is a crucial tool for diagnosing the gastrointestinal tract, but its effectiveness is often limited by a narrow field of view and the dynamic nature of the internal environment, especially in the esophagus, where complex and…

Computer Vision and Pattern Recognition · Computer Science 2024-10-03 Muyang Li , Juming Xiong , Ruining Deng , Tianyuan Yao , Regina N Tyree , Girish Hiremath , Yuankai Huo

In vivo application of dynamic optical coherence tomography (DOCT) is hindered by bulk motion of the sample. We demonstrate DOCT imaging of \invivo human skin by adopting a sample-fixation attachment to suppress bulk motion and a subsequent…

Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful…

The application of the non conventional imaging technique LOFI (Laser Optical Feedback Imaging) to coherent microscopy is presented. This simple and efficient technique using frequency-shifted optical feedback needs the sample to be scanned…

Several techniques have been employed for the direct visualization of cytoskeletal filaments and their associated proteins. Total-internal-reflection-fluorescence (TIRF) microscopy has a high signal-to-background ratio, but it suffers from…

Biological Physics · Physics 2022-01-21 Yazgan Tuna , Amer Al-Hiyasat , Jonathon Howard

The segmentation of endoscopic images plays a vital role in computer-aided diagnosis and treatment. The advancements in deep learning have led to the employment of numerous models for endoscopic tumor segmentation, achieving promising…

Computer Vision and Pattern Recognition · Computer Science 2025-02-03 Lingcong Cai , Yun Li , Xiaomao Fan , Kaixuan Song , Ruxin Wang , Wenbin Lei

Optical Coherence Tomography(OCT) is a non-invasive technique capturing cross-sectional area of the retina in micro-meter resolutions. It has been widely used as a auxiliary imaging reference to detect eye-related pathology and predict…

Image and Video Processing · Electrical Eng. & Systems 2022-09-01 Shuo Chen , Da Ma , Sieun Lee , Timothy T. L. Yu , Gavin Xu , Donghuan Lu , Karteek Popuri , Myeong Jin Ju , Marinko V. Sarunic , Mirza Faisal Beg
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