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Fluorescence microscopy is a powerful tool for imaging biological samples with molecular specificity. In contrast, phase microscopy provides label-free measurement of the sample's refractive index (RI), which is an intrinsic optical…

Optics · Physics 2022-01-26 Yi Xue , David Ren , Laura Waller

Aberrations and multiple scattering in biological tissues critically distort light beams into highly complex speckle patterns. In this regard, digital optical phase conjugation (DOPC) is a promising technique enabling in-depth focusing.…

Analysis of microscope images is a tedious work which requires patience and time, usually done manually by the microscopist after data collection. Here we introduce an approach of automatic image analysis, which is based on locally applied…

Image and Video Processing · Electrical Eng. & Systems 2019-12-17 Benedykt R. Jany , Arkadiusz Janas , Franciszek Krok

Focusing light into opaque random or scattering media such as biological tissue is a much sought-after goal for biomedical applications such as photodynamic therapy, optical manipulation, and photostimulation. However, focusing with…

Optics · Physics 2014-03-10 Jian Wei Tay , Puxiang Lai , Yuta Suzuki , Lihong V. Wang

Bayesian image analysis has played a large role over the last 40+ years in solving problems in image noise-reduction, de-blurring, feature enhancement, and object detection. However, these problems can be complex and lead to computational…

Methodology · Statistics 2024-01-15 John Kornak , Karl Young , Eric Friedman

Artificial Intelligence (AI)-powered pathology is a revolutionary step in the world of digital pathology and shows great promise to increase both diagnosis accuracy and efficiency. However, defocus and motion blur can obscure tissue or cell…

Image and Video Processing · Electrical Eng. & Systems 2020-11-25 Cheng Jiang , Jun Liao , Pei Dong , Zhaoxuan Ma , De Cai , Guoan Zheng , Yueping Liu , Hong Bu , Jianhua Yao

Deep frequency modulation interferometry (DFMI) resolves phase ambiguity in absolute distance measurements by jointly estimating two length-encoding parameters: the coarse and unambiguous effective modulation depth ($m$), and the fine but…

Optics · Physics 2026-04-22 Miguel Dovale-Álvarez

Fourier optics, the principle of using Fourier Transformation to understand the functionalities of optical elements, lies at the heart of modern optics, and has been widely applied to optical information processing, imaging, holography etc.…

The emergence of far-field super-resolution microscopy has rejuvenated the possibility for nanoscale imaging. Approaches to far-field super-resolution that utilize point scanning often depends on spatially reducing the size of the focused…

Optics · Physics 2022-06-17 Ning Xu , Guoxuan Liu , Qiaofeng Tan

Modern neural network training relies on piece-wise (sub-)differentiable functions in order to use backpropagation to update model parameters. In this work, we introduce a novel method to allow simple non-differentiable functions at…

Machine Learning · Computer Science 2019-10-29 Jason Ramapuram , Russ Webb

The rapid advancement of generative AI has enabled the mass production of photorealistic synthetic images, blurring the boundary between authentic and fabricated visual content. This challenge is particularly evident in deepfake scenarios…

Computer Vision and Pattern Recognition · Computer Science 2025-09-30 Minsun Jeon , Simon S. Woo

We introduce a single-frame diffractive imaging method called randomized probe imaging (RPI). In RPI, a sample is illuminated by a structured probe field containing speckles smaller than the sample's typical feature size. Quantitative…

Coherent diffractive imaging (CDI) enables lensless imaging with experimental simplicity and a flexible field of view, yet its resolution is fundamentally constrained by the Abbe diffraction limit. To overcome this limitation, we introduce…

Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…

Optics · Physics 2021-07-07 Ivan Vishniakou , Johannes D. Seelig

Differential Dynamic Microscopy (DDM) analyzes traditional real-space microscope images to extract information on sample dynamics in a way akin to light scattering, by decomposing each image in a sequence into Fourier modes, and evaluating…

Soft Condensed Matter · Physics 2017-11-10 Fabio Giavazzi , Paolo Edera , Peter J. Lu , Roberto Cerbino

Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…

Image and Video Processing · Electrical Eng. & Systems 2020-09-17 Fanglin Linda Liu , Grace Kuo , Nick Antipa , Kyrollos Yanny , Laura Waller

We demonstrate a deep learning-based offline autofocusing method, termed Deep-R, that is trained to rapidly and blindly autofocus a single-shot microscopy image of a specimen that is acquired at an arbitrary out-of-focus plane. We…

Image and Video Processing · Electrical Eng. & Systems 2021-01-25 Yilin Luo , Luzhe Huang , Yair Rivenson , Aydogan Ozcan

Imaging techniques such as functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) achieve deep, non-invasive sensing in turbid media, but they are constrained by the photon budget. Wavefront shaping (WFS) can…

Optics · Physics 2026-04-21 Pablo Jara , Arthur Goetschy , Hui Cao , Alexey Yamilov

Unsupervised deep image prior (DIP) addresses shortcomings of training data requirements and limited generalization associated with supervised deep learning. The performance of DIP depends on the network architecture and the stopping point…

For astronomical instruments, accurate knowledge of the optical pointing and coupling are essential to characterize the alignment and performance of (sub-)systems prior to integration and deployment. Ideally, this requires the phase…

Instrumentation and Methods for Astrophysics · Physics 2020-02-11 S. J. C. Yates , K. K. Davis , W. Jellema , J. J. A. Baselmans , A. M. Baryshev