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Single-shot volumetric fluorescence (SVF) imaging offers a significant advantage over traditional imaging methods that require scanning across multiple axial planes as it can capture biological processes with high temporal resolution. The…

Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…

Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles…

Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor…

Image and Video Processing · Electrical Eng. & Systems 2020-10-13 Kyrollos Yanny , Nick Antipa , William Liberti , Sam Dehaeck , Kristina Monakhova , Fanglin Linda Liu , Konlin Shen , Ren Ng , Laura Waller

Super-resolutive 3D tracking, such as PSF engineering or evanescent field imaging has long been used to track microparticles and to enhance the throughput of single molecules force spectroscopic measurements. However, current methods…

The applications of present nanoscopy techniques for live cell imaging are limited by the long sampling time and low emitter density. Here we developed a new single frame wide-field nanoscopy based on ghost imaging via sparsity constraints…

The spatial resolution and fluorescence signal amplitude in stimulated emission depletion (STED) microscopy is limited by the photostability of available fluorophores. Here, we show that negatively-charged silicon vacancy (SiV) centers in…

Optics · Physics 2019-10-25 Yaser Silani , Forrest Hubert , Victor M. Acosta

Photobleaching is a major limitation of superresolution Stimulated Depletion Emission (STED) microscopy. Fast scanning has long been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful…

Biological Physics · Physics 2015-10-07 Yong Wu , Xundong Wu , Rong Lu , Jin Zhang , Ligia Toro , Enrico Stefani

In this paper, we propose a Monocular 3D Single Stage object Detector (M3DSSD) with feature alignment and asymmetric non-local attention. Current anchor-based monocular 3D object detection methods suffer from feature mismatching. To…

Computer Vision and Pattern Recognition · Computer Science 2021-03-25 Shujie Luo , Hang Dai , Ling Shao , Yong Ding

A long-standing challenge in multiple-particle-tracking is the accurate and precise 3D localization of individual particles at close proximity. One established approach for snapshot 3D imaging is point-spread-function (PSF) engineering, in…

Image and Video Processing · Electrical Eng. & Systems 2020-10-01 Elias Nehme , Boris Ferdman , Lucien E. Weiss , Tal Naor , Daniel Freedman , Tomer Michaeli , Yoav Shechtman

Encouraged by the growing availability of pre-trained 2D diffusion models, image-to-3D generation by leveraging Score Distillation Sampling (SDS) is making remarkable progress. Most existing methods combine novel-view lifting from 2D…

Computer Vision and Pattern Recognition · Computer Science 2024-03-18 Pengkun Liu , Yikai Wang , Fuchun Sun , Jiafang Li , Hang Xiao , Hongxiang Xue , Xinzhou Wang

On 3D imaging, light field cameras typically are of single shot, and however, they heavily suffer from low spatial resolution and depth accuracy. In this paper, by employing an optical projector to project a group of single high-frequency…

Image and Video Processing · Electrical Eng. & Systems 2024-04-11 Geyou Zhang , Ce Zhu , Kai Liu , Yipeng Liu

Super-resolution microscopes (such as STED) illuminate samples with a tiny spot, and achieve very high resolution. But structures smaller than the spot cannot be resolved in this way. Therefore, we propose a technique to solve this problem.…

Image and Video Processing · Electrical Eng. & Systems 2019-11-20 Yaohua Xie

We propose a nested Wolter-I mirror design for a neutron condenser, which is based on established X-ray telescope technology. We demonstrate through simulations that it can increase the flux density at the ESS imaging instrument ODIN by up…

The resolution of far-field optical microscopes, which rely on propagating optical modes, is widely believed to be limited because of diffraction to a value on the order of a half-wavelength $\lambda /2$ of the light used. Although…

Materials Science · Physics 2009-11-10 Igor I. Smolyaninov , Christopher C. Davis , Jill Elliott , Anatoly V. Zayats

Single particle imaging (SPI) at X-ray free electron lasers (XFELs) is a technique to determine the 3D structure of nanoscale objects like biomolecules from a large number of diffraction patterns of copies of these objects in random…

Image and Video Processing · Electrical Eng. & Systems 2022-10-20 Abhishek Mall , Kartik Ayyer

Optical microscopy is an essential tool in biology and medicine. Imaging thin, yet non-flat objects in a single shot (without relying on more sophisticated sectioning setups) remains challenging as the shallow depth of field that comes with…

Image and Video Processing · Electrical Eng. & Systems 2020-10-14 Adrian Shajkofci , Michael Liebling

High-resolution phase-contrast 3D imaging using nano-holotomography typically requires collecting multiple tomograms at varying sample-to-detector distances, usually 3 to 4. This multi-distance approach significantly limits temporal…

Magnetic particle imaging (MPI) is an in-vivo imaging method to detect magnetic nanoparticles for blood vessel imaging and molecular target imaging. Compared with conventional molecular imaging devices (such as nuclear medicine imaging PET…

Medical Physics · Physics 2024-04-26 Guang Jia , Zhongwei Bian , Tianshu Li , Shi Bai , Chenxing Hu , Lixuan Zhao , Peng Gao , Tanping Li , Hui Hui , Jie Tian

Currently, there have been many kinds of voxel-based 3D single stage detectors, while point-based single stage methods are still underexplored. In this paper, we first present a lightweight and effective point-based 3D single stage object…

Computer Vision and Pattern Recognition · Computer Science 2020-02-25 Zetong Yang , Yanan Sun , Shu Liu , Jiaya Jia