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Staining is critical to cell imaging and medical diagnosis, which is expensive, time-consuming, labor-intensive, and causes irreversible changes to cell tissues. Recent advances in deep learning enabled digital staining via supervised model…

Image and Video Processing · Electrical Eng. & Systems 2023-03-06 Ziwang Xu , Lanqing Guo , Shuyan Zhang , Alex C. Kot , Bihan Wen

Virtual histology is an emerging field in biomedicine that enables three-dimensional tissue visualization using X-ray micro-computed tomography. However, the method still lacks the specificity of conventional histology, in which parts of…

In histopathology, tissue sections are typically stained using common H&E staining or special stains (MAS, PAS, PASM, etc.) to clearly visualize specific tissue structures. The rapid advancement of deep learning offers an effective solution…

Image and Video Processing · Electrical Eng. & Systems 2025-04-23 Zizhi Chen , Xinyu Zhang , Minghao Han , Yizhou Liu , Ziyun Qian , Weifeng Zhang , Xukun Zhang , Jingwei Wei , Lihua Zhang

We introduce SynthLight, a diffusion model for portrait relighting. Our approach frames image relighting as a re-rendering problem, where pixels are transformed in response to changes in environmental lighting conditions. Using a…

Computer Vision and Pattern Recognition · Computer Science 2025-01-17 Sumit Chaturvedi , Mengwei Ren , Yannick Hold-Geoffroy , Jingyuan Liu , Julie Dorsey , Zhixin Shu

Imaging mass spectrometry (IMS) is a powerful tool for untargeted, highly multiplexed molecular mapping of tissue in biomedical research. IMS offers a means of mapping the spatial distributions of molecular species in biological tissue with…

Computer Vision and Pattern Recognition · Computer Science 2025-08-05 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Lukasz G. Migas , Raf Van de Plas , Jeffrey M. Spraggins , Aydogan Ozcan

Wavefront-shaping is a promising approach for imaging fluorescent targets deep inside scattering tissue despite strong aberrations. It enables focusing an incoming illumination into a single spot inside tissue, as well as correcting the…

Optics · Physics 2024-01-23 Dror Aizik , Anat Levin

Until recently, conventional biochemical staining had the undisputed status as well-established benchmark for most biomedical problems related to clinical diagnostics, fundamental research and biotechnology. Despite this role as…

In life sciences, fluorescent labeling techniques are used to study molecular structures and interactions of cells. However, this type of cell imaging has its own limitations, one of which is that the process of staining the cells could be…

Image and Video Processing · Electrical Eng. & Systems 2020-05-07 Eunjae Kim

Polarization of the mammalian embryo at the right developmental time is critical for its development to term and would be valuable in assessing the potential of human embryos. However, tracking polarization requires invasive fluorescence…

Quantitative Methods · Quantitative Biology 2021-11-10 Cheng Shen , Adiyant Lamba , Meng Zhu , Ray Zhang , Changhuei Yang , Magdalena Zernicka Goetz

Three-dimensional (3D) histopathology of unprocessed tissues has the potential to transform disease management by enabling volumetric characterization of tissue microarchitecture and in-vivo assessment. Back-illumination Interference…

Computer Vision and Pattern Recognition · Computer Science 2026-05-22 Anthony Song , Boyan Zhou , Mayank Golhar , Marisa Morakis , Alex Baras , Nicholas Durr

Multiple instance learning (MIL) is the dominant framework for whole-slide image analysis in computational pathology, typically combining a frozen patch encoder, a projection layer, and a slide-level aggregator. While encoders and…

Quantitative Methods · Quantitative Biology 2026-05-19 Yucheng Xing , Pei Liu , Jingying Ma , Ruping Hong , Jiangdong Qiu , Tianyu Liu , Kai He , Ling Huang , Mengling Feng

We present a rapid, large-field bimodal imaging platform that integrates conventional brightfield microscopy with a lensless IR imaging scanner, enabling whole-slide IR image stack acquisition in minutes. Using a dedicated deep learning…

Modern histopathology relies on the microscopic examination of thin tissue sections stained with histochemical techniques, typically using brightfield or fluorescence microscopy. However, the staining of samples can permanently alter their…

Phase is a fundamental resource for optical imaging but cannot be directly observed with intensity measurements. The existing methods to quantify a phase distribution rely on complex devices and structures. Here we experimentally…

Optics · Physics 2020-03-25 Tengfeng Zhu , Junyi Huang , Zhichao Ruan

Image simulation remains under-exploited for the most widely used biological phase microscopy methods, because of difficulties in simulating partially coherent illumination. We describe an open-source toolbox, microlith…

Optics · Physics 2014-05-14 Shalin B. Mehta , Rudolf Oldenbourg

Using a deep neural network, we demonstrate a digital staining technique, which we term PhaseStain, to transform quantitative phase images (QPI) of labelfree tissue sections into images that are equivalent to brightfield microscopy images…

Image and Video Processing · Electrical Eng. & Systems 2019-02-08 Yair Rivenson , Tairan Liu , Zhensong Wei , Yibo Zhang , Aydogan Ozcan

Mixed Reality scene relighting, where virtual changes to lighting conditions realistically interact with physical objects, producing authentic illumination and shadows, can be used in a variety of applications. One such application in real…

Graphics · Computer Science 2025-08-22 Hanwen Zhao , John Akers , Baback Elmieh , Ira Kemelmacher-Shlizerman

Structured Illumination Microscopy is a widespread methodology to image live and fixed biological structures smaller than the diffraction limits of conventional optical microscopy. Using recent advances in image up-scaling through deep…

Image and Video Processing · Electrical Eng. & Systems 2021-02-19 Miguel Boland , Edward A. K. Cohen , Seth Flaxman , Mark A. A. Neil

Fluorescence microscopy allows for a detailed inspection of cells, cellular networks, and anatomical landmarks by staining with a variety of carefully-selected markers visualized as color channels. Quantitative characterization of…

Computer Vision and Pattern Recognition · Computer Science 2021-08-26 Alvaro Gomariz , Tiziano Portenier , Patrick M. Helbling , Stephan Isringhausen , Ute Suessbier , César Nombela-Arrieta , Orcun Goksel

Virtual staining streamlines traditional staining procedures by digitally generating stained images from unstained or differently stained images. While conventional staining methods involve time-consuming chemical processes, virtual…