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Double staining in histopathology, particularly for metaplastic breast cancer, typically employs H&E and P63 dyes. However, P63's tissue damage and high cost necessitate alternative methods. This study introduces xAI-CycleGAN, an advanced…

Image and Video Processing · Electrical Eng. & Systems 2023-12-07 Tibor Sloboda , Lukáš Hudec , Wanda Benešová

While many unsupervised learning models focus on one family of tasks, either generative or discriminative, we explore the possibility of a unified representation learner: a model which uses a single pre-training stage to address both…

Computer Vision and Pattern Recognition · Computer Science 2023-07-18 Soumik Mukhopadhyay , Matthew Gwilliam , Vatsal Agarwal , Namitha Padmanabhan , Archana Swaminathan , Srinidhi Hegde , Tianyi Zhou , Abhinav Shrivastava

Multiplex imaging is revolutionizing pathology by enabling the simultaneous visualization of multiple biomarkers within tissue samples, providing molecular-level insights that traditional hematoxylin and eosin (H&E) staining cannot provide.…

Image and Video Processing · Electrical Eng. & Systems 2026-01-06 Hyun-Jic Oh , Junsik Kim , Zhiyi Shi , Yichen Wu , Yu-An Chen , Peter K Sorger , Hanspeter Pfister , Won-Ki Jeong

In the course of the past few years, diffusion models (DMs) have reached an unprecedented level of visual quality. However, relatively little attention has been paid to the detection of DM-generated images, which is critical to prevent…

Computer Vision and Pattern Recognition · Computer Science 2024-01-23 Jonas Ricker , Simon Damm , Thorsten Holz , Asja Fischer

The use of accurate scanning transmission electron microscopy (STEM) image simulation methods require large computation times that can make their use infeasible for the simulation of many images. Other simulation methods based on linear…

Computer Vision and Pattern Recognition · Computer Science 2022-11-18 Nick Lawrence , Mingren Shen , Ruiqi Yin , Cloris Feng , Dane Morgan

Virtual staining of tissue offers a powerful tool for transforming label-free microscopy images of unstained tissue into equivalents of histochemically stained samples. This study presents a diffusion model-based super-resolution virtual…

Image and Video Processing · Electrical Eng. & Systems 2025-07-01 Yijie Zhang , Luzhe Huang , Nir Pillar , Yuzhu Li , Hanlong Chen , Aydogan Ozcan

Computational histopathology image diagnosis becomes increasingly popular and important, where images are segmented or classified for disease diagnosis by computers. While pathologists do not struggle with color variations in slides,…

Image and Video Processing · Electrical Eng. & Systems 2020-07-27 Hanwen Liang , Konstantinos N. Plataniotis , Xingyu Li

Generative virtual staining (VS) models for high-throughput screening (HTS) can provide an estimated posterior distribution of possible biological feature values for each input and cell. However, when evaluating a VS model, the true…

Machine Learning · Computer Science 2026-02-27 Samuel Tonks , Steve Hood , Ryan Musso , Ceridwen Hopely , Steve Titus , Minh Doan , Iain Styles , Alexander Krull

An open secret in contemporary machine learning is that many models work beautifully on standard benchmarks but fail to generalize outside the lab. This has been attributed to biased training data, which provide poor coverage over real…

Computer Vision and Pattern Recognition · Computer Science 2020-02-18 Ali Jahanian , Lucy Chai , Phillip Isola

Staining is critical to cell imaging and medical diagnosis, which is expensive, time-consuming, labor-intensive, and causes irreversible changes to cell tissues. Recent advances in deep learning enabled digital staining via supervised model…

Image and Video Processing · Electrical Eng. & Systems 2023-03-06 Ziwang Xu , Lanqing Guo , Shuyan Zhang , Alex C. Kot , Bihan Wen

The automated analysis of medical images is currently limited by technical and biological noise and bias. The same source tissue can be represented by vastly different images if the image acquisition or processing protocols vary. For an…

The lack of sufficiently large open medical databases is one of the biggest challenges in AI-powered healthcare. Synthetic data created using Generative Adversarial Networks (GANs) appears to be a good solution to mitigate the issues with…

Image and Video Processing · Electrical Eng. & Systems 2023-08-03 Sandra Carrasco Limeros , Sylwia Majchrowska , Mohamad Khir Zoubi , Anna Rosén , Juulia Suvilehto , Lisa Sjöblom , Magnus Kjellberg

Large annotated datasets are required for training deep learning models, but in medical imaging data sharing is often complicated due to ethics, anonymization and data protection legislation. Generative AI models, such as generative…

Image and Video Processing · Electrical Eng. & Systems 2024-01-08 Muhammad Usman Akbar , Måns Larsson , Anders Eklund

Despite the ever-increasing interest in applying deep learning (DL) models to medical imaging, the typical scarcity and imbalance of medical datasets can severely impact the performance of DL models. The generation of synthetic data that…

Image and Video Processing · Electrical Eng. & Systems 2023-04-03 Pouria Rouzrokh , Bardia Khosravi , Shahriar Faghani , Mana Moassefi , Sanaz Vahdati , Bradley J. Erickson

One highly promising direction for enabling flexible real-time on-device image editing is utilizing data distillation by leveraging large-scale text-to-image diffusion models to generate paired datasets used for training generative…

Computer Vision and Pattern Recognition · Computer Science 2024-06-04 Yifan Gong , Zheng Zhan , Qing Jin , Yanyu Li , Yerlan Idelbayev , Xian Liu , Andrey Zharkov , Kfir Aberman , Sergey Tulyakov , Yanzhi Wang , Jian Ren

Virtual immunohistochemistry (IHC) staining from hematoxylin and eosin (H&E) images can accelerate diagnostics by providing preliminary molecular insight directly from routine sections, reducing the need for repeat sectioning when tissue is…

Computer Vision and Pattern Recognition · Computer Science 2026-03-16 Jillur Rahman Saurav , Thuong Le Hoai Pham , Pritam Mukherjee , Paul Yi , Brent A. Orr , Jacob M. Luber

Conditional generative adversarial networks (cGANs) have enabled high-fidelity computational staining and destaining of hematoxylin and eosin (H&E) in digital pathology whole-slide images (WSI). However, their ability to generalize to…

Computer Vision and Pattern Recognition · Computer Science 2026-05-15 Aarushi Kulkarni , Alarice Lowe , Pratik Shah

Deep learning models that are trained on histopathological images obtained from a single lab and/or scanner give poor inference performance on images obtained from another scanner/lab with a different staining protocol. In recent years,…

Image and Video Processing · Electrical Eng. & Systems 2020-10-07 Harshal Nishar , Nikhil Chavanke , Nitin Singhal

Generative adversarial networks (GANs) are frequently utilized in astronomy to construct an emulator of numerical simulations. Nevertheless, training GANs can prove to be a precarious task, as they are prone to instability and often lead to…

Instrumentation and Methods for Astrophysics · Physics 2023-11-14 Xiaosheng Zhao , Yuan-Sen Ting , Kangning Diao , Yi Mao

Spatial Transcriptomics (ST) allows a high-resolution measurement of RNA sequence abundance by systematically connecting cell morphology depicted in Hematoxylin and Eosin (H&E) stained histology images to spatially resolved gene…

Computer Vision and Pattern Recognition · Computer Science 2025-03-21 Sichen Zhu , Yuchen Zhu , Molei Tao , Peng Qiu