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Related papers: Chemically active droplets in crowded environments

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The formation of condensates is now considered as a major organization principle of eukaryotic cells. Several studies have recently shown that the properties of these condensates are affected by enzymatic reactions. We propose here a simple…

Soft Condensed Matter · Physics 2024-11-19 Jacques Fries , Javier Diaz , Marie Jardat , Ignacio Pagonabarraga , Pierre Illien , Vincent Dahirel

Effect of molecular crowding and confinement experienced by protein in the cell during unfolding has been studied by modeling a linear polymer chain on a percolation cluster. It is known that internal structure of the cell changes in time,…

Soft Condensed Matter · Physics 2015-05-14 Amit Raj Singh , Debaprasad Giri , Sanjay Kumar

Liquid-liquid phase separation is important across biology, physics, and materials science. Although usually studied at equilibrium, active components - such as motor proteins, enzymes, and synthetic microswimmers - are increasingly…

Soft Condensed Matter · Physics 2026-01-06 Kan Chang , Yulin Li , Ming Yuan , Masaki Sano , Zhihong You , H. P. Zhang

Phase separation and transitions among different molecular states are ubiquitous in living cells. Such transitions can be governed by local equilibrium thermodynamics or by active processes controlled by biological fuel. It remains largely…

Biological Physics · Physics 2021-11-24 G. Bartolucci , O. Adame-Arana , X. Zhao , C. A. Weber

In the cell, protein complexes form relying on specific interactions between their monomers. Excluded volume effects due to molecular crowding would lead to correlations between molecules even without specific interactions. What is the…

Soft Condensed Matter · Physics 2015-05-13 Wei Wang , Wei-Xin Xu , Y. Levy , E. Trizac , P. G. Wolynes

Intracellular transport of vesicular cargos, organelles, and other macromolecules is an essential process to move large items through a crowded, and inhomogeneous cellular environment. In an effort to dissect the fundamental effects of…

Biomolecules · Quantitative Biology 2014-09-12 Leslie Conway , Jennifer L. Ross

Active transport of biomolecular condensates and cell migration in collectives are fundamental to development, homeostasis, and processes such as cancer progression, wound healing, and infection response. Yet how these assemblies are…

Soft Condensed Matter · Physics 2025-10-24 Hossein Vahid , Jens-Uwe Sommer , Abhinav Sharma

We study the behaviour of catalytically active droplets in multi-component conserved mixtures affected by noise. Working in the thin interface limit, we analytically determine the state diagram of the system, characterized by multiple…

Soft Condensed Matter · Physics 2025-12-01 Jacopo Romano , Martin Kjøllesdal Johnsrud , Benoît Mahault , Ramin Golestanian

Protein dynamics in cells may be different from that in dilute solutions in vitro since the environment in cells is highly concentrated with other macromolecules. This volume exclusion due to macromolecular crowding is predicted to affect…

Biomolecules · Quantitative Biology 2009-11-13 Dirar Homouz , Loren Stagg , Pernilla Wittung-Stafshede , Margaret S. Cheung

A statistical mechanical theory is presented to predict the effects of macromolecular crowding on protein association equilibria, accounting for both excluded volume and attractive interactions between proteins and crowding molecules.…

Soft Condensed Matter · Physics 2015-06-11 Young C. Kim , Jeetain Mittal

The cellular environment, characterized by its intricate composition and spatial organization, hosts a variety of organelles, ranging from membrane-bound ones to membraneless structures that are formed through liquid-liquid phase…

Soft Condensed Matter · Physics 2026-03-09 Gregor Häfner , Marcus Müller

Experiments show that macromolecular crowding modestly reduces the size of intrinsically disordered proteins (IDPs) even at volume fraction ($\phi$) similar to that in the cytosol whereas DNA undergoes a coil-to-globule transition at very…

Soft Condensed Matter · Physics 2016-12-28 Hongsuk Kang , Philip A. Pincus , Changbong Hyeon , D. Thirumalai

Biomolecular condensates in cells are often rich in catalytically-active enzymes. This is particularly true in the case of the large enzymatic complexes known as metabolons, which contain different enzymes that participate in the same…

Subcellular Processes · Quantitative Biology 2021-10-20 Vincent Ouazan-Reboul , Jaime Agudo-Canalejo , Ramin Golestanian

The looping of polymers such as DNA is a fundamental process in the molecular biology of living cells, whose interior is characterised by a high degree of molecular crowding. We here investigate in detail the looping dynamics of flexible…

Soft Condensed Matter · Physics 2014-12-24 Jaeoh Shin , Andrey G. Cherstvy , Ralf Metzler

Phase separating systems that are maintained away from thermodynamic equilibrium via molecular processes represent a class of active systems, which we call active emulsions. These systems are driven by external energy input for example…

Soft Condensed Matter · Physics 2019-06-07 Christoph A. Weber , David Zwicker , Frank Jülicher , Chiu Fan Lee

Microtubules are an essential physical building block of cellular systems. They are organized using specific crosslinkers, motors, and influencers of nucleation and growth. With the addition of anti-parallel crosslinkers, microtubule…

Biological Physics · Physics 2021-06-23 Sumon Sahu , Lena Herbst , Ryan Quinn , Jennifer L. Ross

One of the hypothesized functions of biomolecular condensates is to act as chemical reactors, where chemical reactions can be modulated, i.e. accelerated or slowed down, while substrate molecules enter and products exit from the condensate.…

Biological Physics · Physics 2024-07-02 Alexandra Shelest , Hugo Le Roy , Daniel M. Busiello , Paolo De Los Rios

Driven chemical reactions can control the macroscopic properties of droplets, like their size. Such active droplets are critical in structuring the interior of biological cells. Cells also need to control where and when droplets appear, so…

Soft Condensed Matter · Physics 2024-03-15 Noah Ziethen , Jan Kirschbaum , David Zwicker

Despite the diversity of materials designated as active matter, virtually all active systems undergo a form of dynamic arrest when crowding and activity compete, reminiscent of the dynamic arrest observed in colloidal and molecular fluids…

Soft Condensed Matter · Physics 2019-05-28 Ludovic Berthier , Elijah Flenner , Grzegorz Szamel

It has been proposed that during the early steps in the origin of life, small droplets could have formed via the segregation of molecules from complex mixtures by phase separation. These droplets could have provided chemical reaction…

Biological Physics · Physics 2017-04-26 David Zwicker , Rabea Seyboldt , Christoph A. Weber , Anthony A. Hyman , Frank Jülicher