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Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample excitation.…

We developed a Bessel light sheet fluorescence microscopy (LSFM) system to enable high-speed, wide-field intra-vital imaging of zebrafish and other thick biological samples. This system uses air objectives for the convenient mounting of…

Optics · Physics 2023-11-07 Chuhui Wang , Jiaju Chen , Cuiyi Peng , Zhenglin Chen , Dongmei Yu , Peiwu Qin

We study the effect of finite temperatures and Fermion density on the effective pseudo-scalar-photon vertex induced by the triangle diagram. The manifestly covariant calculations show that when the pseudo-scalar mass is much less than the…

High Energy Physics - Phenomenology · Physics 2007-05-23 Sourendu Gupta , S. N. Nayak

The excitation of microresonators using focused intensity modulated light, known as photothermal excitation, is gaining significant attention due to its capacity to accurately excite microresonators without distortions, even in liquid…

Light-sheet fluorescence microscopy (LSFM) enables real-time whole-brain functional imaging in zebrafish larvae. Conventional one photon LSFM can however induce undesirable visual stimulation due to the use of visible excitation light. The…

We study an inverse problem for Light Sheet Fluorescence Microscopy (LSFM), where the density of fluorescent molecules needs to be reconstructed. Our first step is to present a mathematical model to describe the measurements obtained by an…

Analysis of PDEs · Mathematics 2020-08-26 Evelyn Cueva , Matias Courdurier , Axel Osses , Victor Castañeda , Benjamin Palacios , Steffen Härtel

Weak gravitational lensing is one of the most powerful tools for cosmology, while subject to challenges in quantifying subtle systematic biases. The Point Spread Function (PSF) can cause biases in weak lensing shear inference when the PSF…

Instrumentation and Methods for Astrophysics · Physics 2021-12-13 Tianqing Zhang , Rachel Mandelbaum , The LSST Dark Energy Science Collaboration

Light-sheet fluorescence microscopy (LSFM) makes use of a thin plane of light to optically section and image transparent tissues or organisms {\it{in vivo}}, which has the advantages of fast imaging speed and low phototoxicity. In this…

Biological Physics · Physics 2022-09-12 Hong Ye , Xin Xu , Jixiang Wang , Jing Wang , Yi He , Yu Mu , Guohua Shi

Light sheet fluorescence microscopy (LSFM) has transformed the way we visualize biological tissues in three dimensions, offering high-resolution imaging while minimizing photo-induced damage to the samples. Recent breakthroughs in…

Optics · Physics 2025-05-27 Alireza Tabatabaei Mashayekh , Jeremy Witzens

Transmission of high power laser beams through partially absorbing materials modifies the light propagation via a thermally-induced effect known as thermal lensing. This may cause changes in the beam waist position and degrade the beam…

In this paper we show how response function corrections to shear measurements (e.g. as required by Metacalibration) propagate into cosmic shear power spectra. We investigate a 2-sphere pixel (also known as HEALpixel') correction and a…

Cosmology and Nongalactic Astrophysics · Physics 2023-03-01 T. D. Kitching , N. Tessore , P. L. Taylor

Micro-Raman spectroscopy has become an important tool in probing thermophysical behavior in emerging functional materials such as two-dimensional (2D) layered structures. Localized heating by the focused Raman excitation laser beam is…

Mesoscale and Nanoscale Physics · Physics 2022-11-10 Peter Sokalski , Zherui Han , Gabriella Coloyan Fleming , Brandon Smith , Sean E. Sullivan , Rui Huang , Xiulin Ruan , Li Shi

Multiphoton microscopy is widely used for live imaging. However, its acquisition speed remains limited by fluorophore emission rates and photodamage. To increase the pixel rate of a two-photon microscope beyond a few megahertz (MHz),…

Weak lensing effects are known to introduce non-linear couplings in CMB temperature maps. In inflationary scenario, the primary CMB anisotropies are expected to form a 2D Gaussian map, for which, the probability distribution function of the…

Astrophysics · Physics 2011-05-23 F. Bernardeau

Transient Photoluminescence Microscopy (TPLM) allows for the direct visualization of carrier transport in semiconductor materials with sub nanosecond and few nanometer resolution. The technique is based on measuring changes in the spatial…

We develop a comprehensive approach to simulate the deformation of mirrors and lenses due to thermal and mechanical stresses that couples efficiently to photon-based optics simulations. This expands upon previous work where we demonstrated…

Instrumentation and Methods for Astrophysics · Physics 2019-03-20 J. R. Peterson , E. Peng , C. J. Burke , G. Sembroski , J. Cheng

In this work we present results of the analysis of thermal properties of high-power laser bars obtained by spatially resolved thermoreflectance (TR) spectroscopy. Thermoreflectance is a modulation technique relying on periodic facet…

Materials Science · Physics 2015-05-13 D. Pierscinska , K. Pierscinski , M. Bugajski , J. W. Tomm

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

Thermo-optical effects cause a bifocusing of incoming beams in optical media, due to the birefringence created by a thermal lens that can resolve the incoming beams into two-component signals of different polarizations. We propose a…

Pattern Formation and Solitons · Physics 2011-05-24 Alain M. Dikande

The main advantage of two-photon fluorescence confocal microscopy is the low absorption obtained with live tissues at the wavelengths of operation. However, the resolution of two-photon fluorescence confocal microscopes is lower than in the…

Biological Physics · Physics 2009-11-13 N. Sandeau , H. Giovannini
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