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Super-resolution microscopy is rapidly gaining importance as an analytical tool in the life sciences. A compelling feature is the ability to label biological units of interest with fluorescent markers in living cells and to observe them…

Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

The Fluorescence Detector (FD) of the Pierre Auger Observatory provides a nearly calorimetric measurement of the primary particle energy, since the fluorescence light produced is proportional to the energy dissipated by an Extensive Air…

Instrumentation and Methods for Astrophysics · Physics 2019-08-13 Laura Valore

Spectral properties of fluorescent radiation from a two-level quantum system with broken inversion spatial symmetry, which can be described by a model of an one-electron two-level atom whose electric dipole moment operator has permanent…

Quantum Physics · Physics 2024-12-17 Nikolai N. Bogolyubov, , Andrey V. Soldatov

Fluorescence Lifetime Imaging Microscopy in the time domain is typically performed by recording the arrival time of photons either by using electronic time tagging or a gated detector. As such the temporal resolution is limited by the…

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, established methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or…

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…

Short wavelength Free-Electron Lasers (FELs) are the newest light sources available to scientists to probe a wide range of phenomena, with chemical, physical and biological applications, using soft and hard X-rays. These sources include the…

Fourier transform spectroscopy (FTS) has been widely used as an analytical tool for many applications in science and engineering. In this paper, we describe the operation principle and practical implementation of an FTS prototype. First,…

Instrumentation and Detectors · Physics 2023-02-02 Sohrab Sheikh-Sofla , Mohammad Neshat

Optical spectroscopy is an important and widely used technique, for instance, to characterize new materials and to identify unknown compounds. Spectra are typically reported as a function of the wavelength of light, yet the information…

Optics · Physics 2026-05-06 Erik F. Woering , Richard Hildner

Fluorescence microscopy has revolutionized biomedical research over the past three decades. Its high molecular specificity and unrivaled single molecule level sensitivity have enabled breakthroughs in a variety of research fields. For in…

Optics · Physics 2012-03-21 Meng Cui , Ke Si , Reto Fiolka

This document briefly introduces the key concepts to understand the phenomenon of fluorescence enhancement with optical nanostructures. The equations remain simple, with the major goal to illustrate the different physical effects at play.…

Chemical Physics · Physics 2017-09-21 Jerome Wenger

This study investigates the propagation of an ultrafast laser pulse through a liquid medium. A femtosecond laser oscillator with a pulse duration of less than 8 fs is used. By conducting experiments with coumarin and fluorescein dyes in…

The random motion of molecules in living cells has consistently been reported to deviate from standard Brownian motion, a behavior coined as ``anomalous diffusion''. Fluorescence Correlation Spectroscopy (FCS) is a powerful method to…

Quantitative Methods · Quantitative Biology 2024-07-18 Nathan Quiblier , Jan-Michael Rye , Pierre Leclerc , Henri Truong , Abdelkrim Hannou , Laurent Héliot , Hugues Berry

We review the continuous monitoring of a qubit through its spontaneous emission, at an introductory level. Contemporary experiments have been able to collect the fluorescence of an artificial atom in a cavity and transmission line, and then…

Quantum Physics · Physics 2020-09-28 Philippe Lewalle , Sreenath K. Manikandan , Cyril Elouard , Andrew N. Jordan

The movement of molecules inside living cells is a fundamental feature of biological processes. The ability to both observe and analyse the details of molecular diffusion in vivo at the single molecule and single cell level can add…

Quantitative Methods · Quantitative Biology 2012-11-21 Alex Robson , Kevin Burrage , Mark Leake

For the first time, complete source distributions for the emission and absorption of acoustic and electromagnetic wavelets are defined and computed, both in spacetime and Fourier space. The biggest surprise is the great simplicity of the…

Mathematical Physics · Physics 2009-11-10 Gerald Kaiser

Based on diffraction theory and the propagation of the light, Fourier optics is a powerful tool allowing the estimation of a visible-range imaging system to transfer the spatial frequency components of an object. The analyses of the imaging…

General Physics · Physics 2018-06-05 Stephane Perrin , Paul Montgomery

Fluorescence telescopes are among the key instruments used for studying ultra-high energy cosmic rays in all modern experiments. We use model data for a small ground-based telescope EUSO-TA to try some methods of machine learning and neural…

Instrumentation and Methods for Astrophysics · Physics 2025-07-08 Mikhail Zotov , Pavel Zakharov

The phasor field has been shown to be a valuable tool for non-line-of-sight imaging. We present a formal analysis of phasor-field imaging using paraxial wave optics. Then, we derive a set of propagation primitives---using the two-frequency,…

Optics · Physics 2019-07-24 Justin Dove , Jeffrey H. Shapiro