Related papers: Five-dimensional single-shot fluorescence imaging …
We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…
Fourier ptychographic microscopy (FPM) is a recently developed imaging modality that uses angularly varying illumination to extend a system performance beyond the limit defined by its optical elements. The FPM technique applies a novel…
Light field microscopy (LFM) uses a microlens array (MLA) near the sensor plane of a microscope to achieve single-shot 3D imaging of a sample without any moving parts. Unfortunately, the 3D capability of LFM comes with a significant loss of…
Recently introduced speckle-correlations based techniques enable noninvasive imaging of objects hidden behind scattering layers. In these techniques the hidden object Fourier amplitude is retrieved from the scattered light autocorrelation,…
Snapshot multi-dimensional imaging offers a promising alternative to traditional low-dimensional imaging techniques by enabling the simultaneous capture of spatial, spectral, polarization, and other information in a single shot for improved…
The extraction of information carried by light plays an increasingly important role in optical communication, imaging, and detection. However, the information can only be successfully extracted when the light pulse is comparably strong,…
FRET-based approaches are a unique tool for sensing the immediate surroundings and interactions of (bio)molecules. FRET imaging and FLIM (Fluorescence Lifetime Imaging Microscopy) enable the visualization of the spatial distribution of…
Light-sheet microscopy (LSM) is a powerful imaging technique that uses a planar illumination oriented orthogonally to the detection axis. Two-photon (2P) LSM is a variant of LSM that exploits the 2P absorption effect for sample excitation.…
The scattering of multispectral incoherent light is a common and unfavorable signal scrambling in natural scenes. However, the blurred light spot due to scattering still holds lots of information remaining to be explored. Former methods…
We propose a method of complex-amplitude Fourier single-pixel imaging (CFSI) with coherent structured illumination to acquire both the amplitude and phase of an object. In the proposed method, an object is illustrated by a series of…
Polarized light microscopy provides unique opportunities for analyzing the molecular order in man-made and natural materials, including biological structures inside living cells, tissues, and whole organisms. 20 years ago, the LC-PolScope…
In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field,…
The novel camera architecture facilitates the development of machine vision. Instead of capturing frame sequences in the temporal domain as traditional video cameras, FourierCam directly measures the pixel-wise temporal spectrum of the…
We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…
Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…
States of light encoding multiple polarizations - vector beams - offer unique capabilities in metrology and communication. However, their practical application is limited by the lack of methods for measuring many polarizations in a scalable…
We present a novel approach for imaging through scattering media that combines the principles of Fourier spatial filtering and single-pixel imaging. We compare the performance of our single-pixel imaging setup with that of a conventional…
We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…
This paper presents an application of digital Fourier holography for selective imaging of scatterers with different sizes in turbid media such as biological tissues. A combination of Fourier holography and high-resolution digital recording,…
Multispectral polarisation imaging has a broad range of applications, from biological cell imaging to agricultural remote surveying. For such applications, especially involving lightweight unmanned aerial vehicles like drones, it is…